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Title: Cusp-artifacts in high order superresolution optical fluctuation imaging

Abstract

Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique, and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify “cusp artifacts” in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes, and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.

Authors:
; ORCiD logo
Publication Date:
Research Org.:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1580802
Alternate Identifier(s):
OSTI ID: 1643763
Report Number(s):
LLNL-JRNL-799072
Journal ID: ISSN 2156-7085
Grant/Contract Number:  
AC52-07NA27344
Resource Type:
Published Article
Journal Name:
Biomedical Optics Express
Additional Journal Information:
Journal Name: Biomedical Optics Express Journal Volume: 11 Journal Issue: 2; Journal ID: ISSN 2156-7085
Publisher:
Optical Society of America
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Yi, Xiyu, and Weiss, Shimon. Cusp-artifacts in high order superresolution optical fluctuation imaging. United States: N. p., 2020. Web. doi:10.1364/BOE.382296.
Yi, Xiyu, & Weiss, Shimon. Cusp-artifacts in high order superresolution optical fluctuation imaging. United States. doi:10.1364/BOE.382296.
Yi, Xiyu, and Weiss, Shimon. Thu . "Cusp-artifacts in high order superresolution optical fluctuation imaging". United States. doi:10.1364/BOE.382296.
@article{osti_1580802,
title = {Cusp-artifacts in high order superresolution optical fluctuation imaging},
author = {Yi, Xiyu and Weiss, Shimon},
abstractNote = {Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique, and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify “cusp artifacts” in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes, and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.},
doi = {10.1364/BOE.382296},
journal = {Biomedical Optics Express},
number = 2,
volume = 11,
place = {United States},
year = {2020},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1364/BOE.382296

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Cited by: 3 works
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