Cusp-artifacts in high order superresolution optical fluctuation imaging
Abstract
Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique, and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify “cusp artifacts” in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes, and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1580802
- Alternate Identifier(s):
- OSTI ID: 1643763
- Report Number(s):
- LLNL-JRNL-799072
Journal ID: ISSN 2156-7085
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Published Article
- Journal Name:
- Biomedical Optics Express
- Additional Journal Information:
- Journal Name: Biomedical Optics Express Journal Volume: 11 Journal Issue: 2; Journal ID: ISSN 2156-7085
- Publisher:
- Optical Society of America
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Yi, Xiyu, and Weiss, Shimon. Cusp-artifacts in high order superresolution optical fluctuation imaging. United States: N. p., 2020.
Web. doi:10.1364/BOE.382296.
Yi, Xiyu, & Weiss, Shimon. Cusp-artifacts in high order superresolution optical fluctuation imaging. United States. https://doi.org/10.1364/BOE.382296
Yi, Xiyu, and Weiss, Shimon. Thu .
"Cusp-artifacts in high order superresolution optical fluctuation imaging". United States. https://doi.org/10.1364/BOE.382296.
@article{osti_1580802,
title = {Cusp-artifacts in high order superresolution optical fluctuation imaging},
author = {Yi, Xiyu and Weiss, Shimon},
abstractNote = {Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique, and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify “cusp artifacts” in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes, and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.},
doi = {10.1364/BOE.382296},
journal = {Biomedical Optics Express},
number = 2,
volume = 11,
place = {United States},
year = {Thu Jan 02 00:00:00 EST 2020},
month = {Thu Jan 02 00:00:00 EST 2020}
}
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https://doi.org/10.1364/BOE.382296
https://doi.org/10.1364/BOE.382296
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