Cusp-artifacts in high order superresolution optical fluctuation imaging

Yi, Xiyu; Weiss, Shimon

doi:10.1364/BOE.382296

Title: Cusp-artifacts in high order superresolution optical fluctuation imaging

Journal Article · 01 January 2020 · Biomedical Optics Express

DOI: https://doi.org/10.1364/BOE.382296 · OSTI ID:1580802

Yi, Xiyu;

Superresolution optical fluctuation imaging (SOFI) is a simple and affordable super-resolution imaging technique, and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify “cusp artifacts” in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes, and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.

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Sponsoring Organization:: USDOE

OSTI ID:: 1580802

Journal Information:: Biomedical Optics Express, Journal Name: Biomedical Optics Express Journal Issue: 2 Vol. 11; ISSN 2156-7085

Publisher:: Optical Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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