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Title: Effects of pH on cell growth, lipid production and CO 2 addition of microalgae Chlorella sorokiniana

Abstract

Microalgae have emerged as one of the most promising alternative energy feedstocks. Some advantages include the simple cellular structure, short production cycle, high lipid content, and fast growth. However, high production costs due to high CO 2 usage and low lipid productivity have been some of the major challenges impeding the commercial production of algal biodiesel. Here, cell growth and lipid content of Chlorella sorokiniana DOE1412 were first evaluated at different pH in flask cultivation. Culture pH was manipulated by CO 2 addition. The optimal pH for DOE1412 is approximately 6.0 when only accounting for cell growth and lipid production and not considering the CO 2 efficiency. A flat panel airlift photobioreactor (PBR) was used for scale-up cultivation at five different pH levels (6.5, 7, 7.5, 8 and 8.5). Data of pH values and CO 2 addition was collected by a data logger. Biomass productivity increased with decreasing pH. By taking into account not only the cell growth and lipid production but also CO 2 addition, the lowest value of CO 2 addition was achieved at pH 8 (2.01 g CO 2/g biomass). The fatty acid profiles and biodiesel properties, such as iodine value (IV), saponification value (SV), cetane numbermore » (CN), degree of unsaturation (DU), long-chain saturated factor (LCSF), and cold filter plugging point (CFPP), were determined as a function of pH. CN of biodiesel produced at pH 6.5, 7 and 7.5 satisfied the US standard ASTM D6751; among them, the pH 6.5 products met the Europe standard EN 14214. Finally, protein content in microalgal biomass increased with increasing pH, while C/N ratio in cells decreased.« less

Authors:
 [1];  [1];  [2];  [1]
  1. The Univ. of Arizona, Tucson, AZ (United States)
  2. Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Univ. of Arizona, Tucson, AZ (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1580748
Alternate Identifier(s):
OSTI ID: 1549084
Grant/Contract Number:  
EE0006269
Resource Type:
Accepted Manuscript
Journal Name:
Algal Research
Additional Journal Information:
Journal Volume: 28; Journal Issue: C; Journal ID: ISSN 2211-9264
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Microalgae; Biodiesel; Chlorella sorokiniana; pH; Lipid; Carbon dioxide

Citation Formats

Ogden, Kimberly L., Qiu, Renhe, Lopez, Paola A., and Gao, Song. Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana. United States: N. p., 2017. Web. doi:10.1016/j.algal.2017.11.004.
Ogden, Kimberly L., Qiu, Renhe, Lopez, Paola A., & Gao, Song. Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana. United States. doi:10.1016/j.algal.2017.11.004.
Ogden, Kimberly L., Qiu, Renhe, Lopez, Paola A., and Gao, Song. Wed . "Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana". United States. doi:10.1016/j.algal.2017.11.004. https://www.osti.gov/servlets/purl/1580748.
@article{osti_1580748,
title = {Effects of pH on cell growth, lipid production and CO2 addition of microalgae Chlorella sorokiniana},
author = {Ogden, Kimberly L. and Qiu, Renhe and Lopez, Paola A. and Gao, Song},
abstractNote = {Microalgae have emerged as one of the most promising alternative energy feedstocks. Some advantages include the simple cellular structure, short production cycle, high lipid content, and fast growth. However, high production costs due to high CO2 usage and low lipid productivity have been some of the major challenges impeding the commercial production of algal biodiesel. Here, cell growth and lipid content of Chlorella sorokiniana DOE1412 were first evaluated at different pH in flask cultivation. Culture pH was manipulated by CO2 addition. The optimal pH for DOE1412 is approximately 6.0 when only accounting for cell growth and lipid production and not considering the CO2 efficiency. A flat panel airlift photobioreactor (PBR) was used for scale-up cultivation at five different pH levels (6.5, 7, 7.5, 8 and 8.5). Data of pH values and CO2 addition was collected by a data logger. Biomass productivity increased with decreasing pH. By taking into account not only the cell growth and lipid production but also CO2 addition, the lowest value of CO2 addition was achieved at pH 8 (2.01 g CO2/g biomass). The fatty acid profiles and biodiesel properties, such as iodine value (IV), saponification value (SV), cetane number (CN), degree of unsaturation (DU), long-chain saturated factor (LCSF), and cold filter plugging point (CFPP), were determined as a function of pH. CN of biodiesel produced at pH 6.5, 7 and 7.5 satisfied the US standard ASTM D6751; among them, the pH 6.5 products met the Europe standard EN 14214. Finally, protein content in microalgal biomass increased with increasing pH, while C/N ratio in cells decreased.},
doi = {10.1016/j.algal.2017.11.004},
journal = {Algal Research},
number = C,
volume = 28,
place = {United States},
year = {2017},
month = {11}
}

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Works referencing / citing this record:

Microalgal-Based Carbon Sequestration by Converting LNG-Fired Waste CO2 into Red Gold Astaxanthin: The Potential Applicability
journal, May 2019

  • Hong, Min Eui; Chang, Won Seok; Patel, Anil Kumar
  • Energies, Vol. 12, Issue 9
  • DOI: 10.3390/en12091718