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Title: Untargeted metabolomic profiling of Sphagnum fallax reveals novel antimicrobial metabolites

Abstract

Sphagnum mosses dominate peatlands by employing harsh ecosystem tactics to prevent vascular plant growth and microbial degradation of these large carbon stores. Knowledge about Sphagnum-produced metabolites, their structure and their function, is important to better understand the mechanisms, underlying this carbon sequestration phenomenon in the face of climate variability. It is currently unclear which compounds are responsible for inhibition of organic matter decomposition and the mechanisms by which this inhibition occurs. Metabolite profiling of Sphagnum fallax was performed using two types of mass spectrometry (MS) systems and 1H nuclear magnetic resonance spectroscopy ( 1H NMR). Lipidome profiling was performed using LC-MS/MS. A total of 655 metabolites, including one hundred fifty-two lipids, were detected by NMR and LC-MS/MS—329 of which were novel metabolites (31 unknown lipids). Sphagum fallax metabolite profile was composed mainly of acid-like and flavonoid glycoside compounds, that could be acting as potent antimicrobial compounds, allowing Sphagnum to control its environment. Sphagnum fallax metabolite composition comparison against previously known antimicrobial plant metabolites confirmed this trend, with seventeen antimicrobial compounds discovered to be present in Sphagnum fallax, the majority of which were acids and glycosides. Biological activity of these compounds needs to be further tested to confirm antimicrobial qualities. Threemore » fungal metabolites were identified providing insights into fungal colonization that may benefit Sphagnum. Characterizing the metabolite profile of Sphagnum fallax provided a baseline to understand the mechanisms in which Sphagnum fallax acts on its environment, its relation to carbon sequestration in peatlands, and provide key biomarkers to predict peatland C store changes (sequestration, emissions) as climate shifts.« less

Authors:
 [1];  [1];  [1];  [1];  [2]; ORCiD logo [2];  [2];  [2];  [2]; ORCiD logo [3]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [4]
  1. Univ. of Arizona, Tucson, AZ (United States)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. Bruker Daltonics Inc, Billercia, MA (United States)
  4. Univ. of Arizona, Tucson, AZ (United States); Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1580519
Report Number(s):
PNNL-SA-146621
Journal ID: ISSN 2475-4455; PLD3
Grant/Contract Number:  
AC05-76RL01830; AC05‐00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Plant Direct
Additional Journal Information:
Journal Volume: 3; Journal Issue: 11; Journal ID: ISSN 2475-4455
Publisher:
Wiley and American Society of Plant Biologists and Society for Experimental Biology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; antimicrobial metabolites; fungal metabolites; lipidomics; metabolomics; Sphagnum fallax

Citation Formats

Fudyma, Jane D., Lyon, Jamee, AminiTabrizi, Roya, Gieschen, Hans, Chu, Rosalie K., Hoyt, David W., Kyle, Jennifer E., Toyoda, Jason, Tolic, Nikola, Heyman, Heino M., Hess, Nancy J., Metz, Thomas O., and Tfaily, Malak M. Untargeted metabolomic profiling of Sphagnum fallax reveals novel antimicrobial metabolites. United States: N. p., 2019. Web. doi:10.1002/pld3.179.
Fudyma, Jane D., Lyon, Jamee, AminiTabrizi, Roya, Gieschen, Hans, Chu, Rosalie K., Hoyt, David W., Kyle, Jennifer E., Toyoda, Jason, Tolic, Nikola, Heyman, Heino M., Hess, Nancy J., Metz, Thomas O., & Tfaily, Malak M. Untargeted metabolomic profiling of Sphagnum fallax reveals novel antimicrobial metabolites. United States. doi:10.1002/pld3.179.
Fudyma, Jane D., Lyon, Jamee, AminiTabrizi, Roya, Gieschen, Hans, Chu, Rosalie K., Hoyt, David W., Kyle, Jennifer E., Toyoda, Jason, Tolic, Nikola, Heyman, Heino M., Hess, Nancy J., Metz, Thomas O., and Tfaily, Malak M. Tue . "Untargeted metabolomic profiling of Sphagnum fallax reveals novel antimicrobial metabolites". United States. doi:10.1002/pld3.179. https://www.osti.gov/servlets/purl/1580519.
@article{osti_1580519,
title = {Untargeted metabolomic profiling of Sphagnum fallax reveals novel antimicrobial metabolites},
author = {Fudyma, Jane D. and Lyon, Jamee and AminiTabrizi, Roya and Gieschen, Hans and Chu, Rosalie K. and Hoyt, David W. and Kyle, Jennifer E. and Toyoda, Jason and Tolic, Nikola and Heyman, Heino M. and Hess, Nancy J. and Metz, Thomas O. and Tfaily, Malak M.},
abstractNote = {Sphagnum mosses dominate peatlands by employing harsh ecosystem tactics to prevent vascular plant growth and microbial degradation of these large carbon stores. Knowledge about Sphagnum-produced metabolites, their structure and their function, is important to better understand the mechanisms, underlying this carbon sequestration phenomenon in the face of climate variability. It is currently unclear which compounds are responsible for inhibition of organic matter decomposition and the mechanisms by which this inhibition occurs. Metabolite profiling of Sphagnum fallax was performed using two types of mass spectrometry (MS) systems and 1H nuclear magnetic resonance spectroscopy (1H NMR). Lipidome profiling was performed using LC-MS/MS. A total of 655 metabolites, including one hundred fifty-two lipids, were detected by NMR and LC-MS/MS—329 of which were novel metabolites (31 unknown lipids). Sphagum fallax metabolite profile was composed mainly of acid-like and flavonoid glycoside compounds, that could be acting as potent antimicrobial compounds, allowing Sphagnum to control its environment. Sphagnum fallax metabolite composition comparison against previously known antimicrobial plant metabolites confirmed this trend, with seventeen antimicrobial compounds discovered to be present in Sphagnum fallax, the majority of which were acids and glycosides. Biological activity of these compounds needs to be further tested to confirm antimicrobial qualities. Three fungal metabolites were identified providing insights into fungal colonization that may benefit Sphagnum. Characterizing the metabolite profile of Sphagnum fallax provided a baseline to understand the mechanisms in which Sphagnum fallax acts on its environment, its relation to carbon sequestration in peatlands, and provide key biomarkers to predict peatland C store changes (sequestration, emissions) as climate shifts.},
doi = {10.1002/pld3.179},
journal = {Plant Direct},
number = 11,
volume = 3,
place = {United States},
year = {2019},
month = {11}
}

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