Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering

doi:10.1002/pro.3802

This content will become publicly available on December 25, 2020

Title: Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering

Authors:

Cannon, Kevin A. ^[1]; Park, Rachel U. ^[2]; Boyken, Scott E. ^[3]; Nattermann, Una ^[4]; Yi, Sue ^[3]; Baker, David ^[5]; King, Neil P. ^[3];

^[6]

UCLA‐DOE Institute for Genomics and Proteomics Los Angeles California, UCLA Department of Chemistry and Biochemistry Los Angeles California
University of Washington Institute for Protein Design Seattle Washington
University of Washington Institute for Protein Design Seattle Washington, University of Washington Department of Biochemistry Seattle Washington
University of Washington Institute for Protein Design Seattle Washington, University of Washington Department of Biochemistry Seattle Washington, University of Washington Graduate Program in Biological Physics, Structure &, Design Seattle Washington
University of Washington Institute for Protein Design Seattle Washington, University of Washington Department of Biochemistry Seattle Washington, Howard Hughes Medical Institute Seattle Washington
UCLA‐DOE Institute for Genomics and Proteomics Los Angeles California, UCLA Department of Chemistry and Biochemistry Los Angeles California, UCLA Molecular Biology Institute Los Angeles California

Publication Date:: 2019-12-26

Sponsoring Org.:: USDOE

OSTI Identifier:: 1580448

Grant/Contract Number:: DE‐FC02‐02ER63421

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Protein Science

Additional Journal Information:: Journal Name: Protein Science; Journal ID: ISSN 0961-8368

Publisher:: Wiley Blackwell (John Wiley & Sons)

Country of Publication:: United Kingdom

Language:: English

Citation Formats


                    Cannon, Kevin A., Park, Rachel U., Boyken, Scott E., Nattermann, Una, Yi, Sue, Baker, David, King, Neil P., and Yeates, Todd O. Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering.  United Kingdom: N. p., 2019. 
        Web.  doi:10.1002/pro.3802.

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                    Cannon, Kevin A., Park, Rachel U., Boyken, Scott E., Nattermann, Una, Yi, Sue, Baker, David, King, Neil P., & Yeates, Todd O. Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering.  United Kingdom.  doi:10.1002/pro.3802.

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                    Cannon, Kevin A., Park, Rachel U., Boyken, Scott E., Nattermann, Una, Yi, Sue, Baker, David, King, Neil P., and Yeates, Todd O. Thu .  
        "Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering".  United Kingdom.  doi:10.1002/pro.3802.

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@article{osti_1580448,

  title        = {Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering},

  author       = {Cannon, Kevin A. and Park, Rachel U. and Boyken, Scott E. and Nattermann, Una and Yi, Sue and Baker, David and King, Neil P. and Yeates, Todd O.},

  abstractNote = {},

  doi          = {10.1002/pro.3802},

  journal      = {Protein Science},

  number       = ,

  volume       = ,

  place        = {United Kingdom},

  year         = {2019},

  month        = {12}

}

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DOI: 10.1002/pro.3802

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Similar Records

This content will become publicly available on December 25, 2020

Title: Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering

Citation Formats

Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus journal, March 2019

Flexible, symmetry-directed approach to assembling protein cages journal, July 2016

Accurate design of co-assembling multi-component protein nanomaterials journal, May 2014

Overview of Electron Crystallography of Membrane Proteins: Crystallization and Screening Strategies Using Negative Stain Electron Microscopy journal, April 2013

Structure and Flexibility of Nanoscale Protein Cages Designed by Symmetric Self-Assembly journal, May 2013

EMAN2: An extensible image processing suite for electron microscopy journal, January 2007

Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments journal, February 2001

Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy journal, May 2012

Coiled-Coil-Mediated Assembly of an Icosahedral Protein Cage with Extremely High Thermal and Chemical Stability journal, May 2019

Coot model-building tools for molecular graphics journal, November 2004

Programmable design of orthogonal protein heterodimers journal, December 2018

A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold journal, April 2019

Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression: Designed Protein Tetrahedron journal, August 2015

De novo design of protein homo-oligomers with modular hydrogen-bond network-mediated specificity journal, May 2016

High-symmetry protein assemblies: patterns and emerging applications journal, April 2019

Accurate design of megadalton-scale two-component icosahedral protein complexes journal, July 2016

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Geometric Principles for Designing Highly Symmetric Self-Assembling Protein Nanomaterials journal, May 2017

Porous Protein Crystals as Reaction Vessels journal, June 2013

De novo design of tunable, pH-driven conformational changes journal, May 2019

Porous protein crystals as scaffolds for enzyme immobilization journal, January 2019

Protein cage assembly across multiple length scales journal, January 2018

Design of a hyperstable 60-subunit protein icosahedron journal, June 2016

Symmetry-Directed Self-Assembly of a Tetrahedral Protein Cage Mediated by de Novo-Designed Coiled Coils journal, August 2017

Structure of a 16-nm Cage Designed by Using Protein Oligomers journal, May 2012

Optimal description of a protein structure in terms of multiple groups undergoing TLS motion journal, March 2006

Rational Engineering of a Designed Protein Cage for siRNA Delivery journal, July 2018

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NIH Image to ImageJ: 25 years of image analysis journal, June 2012

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Evolution of a designed protein assembly encapsulating its own RNA genome journal, December 2017

Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus
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journal, July 2016

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journal, May 2014

Overview of Electron Crystallography of Membrane Proteins: Crystallization and Screening Strategies Using Negative Stain Electron Microscopy
journal, April 2013

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journal, May 2019

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A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold
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Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression: Designed Protein Tetrahedron
journal, August 2015

De novo design of protein homo-oligomers with modular hydrogen-bond network-mediated specificity
journal, May 2016

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journal, June 2013

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