3D Ultrastructure of the Cochlear Outer Hair Cell Lateral Wall Revealed By Electron Tomography
Abstract
Outer Hair Cells (OHCs) in the mammalian cochlea display a unique type of voltage-induced mechanical movement termed electromotility, which amplifies auditory signals and contributes to the sensitivity and frequency selectivity of mammalian hearing. Electromotility occurs in the OHC lateral wall, but it is not fully understood how the supramolecular architecture of the lateral wall enables this unique form of cellular motility. Employing electron tomography of high-pressure frozen and freeze-substituted OHCs, we visualized the 3D structure and organization of the membrane and cytoskeletal components of the OHC lateral wall. The subsurface cisterna (SSC) is a highly prominent feature, and we report that the SSC membranes and lumen possess hexagonally ordered arrays of particles. We also find the SSC is tightly connected to adjacent actin filaments by short filamentous protein connections. Pillar proteins that join the plasma membrane to the cytoskeleton appear as variable structures considerably thinner than actin filaments and significantly more flexible than actin-SSC links. The structurally rich organization and rigidity of the SSC coupled with apparently weaker mechanical connections between the plasma membrane (PM) and cytoskeleton reveal that the membrane-cytoskeletal architecture of the OHC lateral wall is more complex than previously appreciated. These observations are important for our understandingmore »
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); National Science Foundation (NSF); National Institutes of Health (NIH)
- OSTI Identifier:
- 1580126
- Alternate Identifier(s):
- OSTI ID: 1599792
- Grant/Contract Number:
- AC02-05CH11231; FG02-97ER25308; AC03-76SF00098; 5F30DC009359; 044379; DC009622; DC0008134; DC07680
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Cellular Neuroscience
- Additional Journal Information:
- Journal Name: Frontiers in Cellular Neuroscience Journal Volume: 13; Journal ID: ISSN 1662-5102
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; outer hair cell (OHC); subsurface cisternae; cortical cytoskeleton; high pressure freezing and freeze substitution; electron tomography (ET)
Citation Formats
Triffo, William Jeffrey, Palsdottir, Hildur, Song, Junha, Morgan, David Gene, McDonald, Kent L., Auer, Manfred, and Raphael, Robert M. 3D Ultrastructure of the Cochlear Outer Hair Cell Lateral Wall Revealed By Electron Tomography. Country unknown/Code not available: N. p., 2019.
Web. doi:10.3389/fncel.2019.00560.
Triffo, William Jeffrey, Palsdottir, Hildur, Song, Junha, Morgan, David Gene, McDonald, Kent L., Auer, Manfred, & Raphael, Robert M. 3D Ultrastructure of the Cochlear Outer Hair Cell Lateral Wall Revealed By Electron Tomography. Country unknown/Code not available. https://doi.org/10.3389/fncel.2019.00560
Triffo, William Jeffrey, Palsdottir, Hildur, Song, Junha, Morgan, David Gene, McDonald, Kent L., Auer, Manfred, and Raphael, Robert M. Fri .
"3D Ultrastructure of the Cochlear Outer Hair Cell Lateral Wall Revealed By Electron Tomography". Country unknown/Code not available. https://doi.org/10.3389/fncel.2019.00560.
@article{osti_1580126,
title = {3D Ultrastructure of the Cochlear Outer Hair Cell Lateral Wall Revealed By Electron Tomography},
author = {Triffo, William Jeffrey and Palsdottir, Hildur and Song, Junha and Morgan, David Gene and McDonald, Kent L. and Auer, Manfred and Raphael, Robert M.},
abstractNote = {Outer Hair Cells (OHCs) in the mammalian cochlea display a unique type of voltage-induced mechanical movement termed electromotility, which amplifies auditory signals and contributes to the sensitivity and frequency selectivity of mammalian hearing. Electromotility occurs in the OHC lateral wall, but it is not fully understood how the supramolecular architecture of the lateral wall enables this unique form of cellular motility. Employing electron tomography of high-pressure frozen and freeze-substituted OHCs, we visualized the 3D structure and organization of the membrane and cytoskeletal components of the OHC lateral wall. The subsurface cisterna (SSC) is a highly prominent feature, and we report that the SSC membranes and lumen possess hexagonally ordered arrays of particles. We also find the SSC is tightly connected to adjacent actin filaments by short filamentous protein connections. Pillar proteins that join the plasma membrane to the cytoskeleton appear as variable structures considerably thinner than actin filaments and significantly more flexible than actin-SSC links. The structurally rich organization and rigidity of the SSC coupled with apparently weaker mechanical connections between the plasma membrane (PM) and cytoskeleton reveal that the membrane-cytoskeletal architecture of the OHC lateral wall is more complex than previously appreciated. These observations are important for our understanding of OHC mechanics and need to be considered in computational models of OHC electromotility that incorporate subcellular features.},
doi = {10.3389/fncel.2019.00560},
journal = {Frontiers in Cellular Neuroscience},
number = ,
volume = 13,
place = {Country unknown/Code not available},
year = {2019},
month = {12}
}
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