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Title: Synthetic fungal multifunctional cellulases for enhanced biomass conversion

Journal Article · · Green Chemistry
DOI: https://doi.org/10.1039/C9GC03062J · OSTI ID:1579978
ORCiD logo [1]; ORCiD logo [1];  [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1]; ORCiD logo [1]
  1. Biosciences Center, National Renewable Energy Lab, Golden, USA
  2. Life Sciences Division, Institute of Advanced Study in Science and Technology (IASST), Guwahati, India
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Synthetic multifunctional enzymes exhibit superior performance to their non-multifunctional enzyme components and exhibit novel cellulose deconstruction mechanisms.

Research Organization:
National Renewable Energy Laboratory (NREL), Golden, CO (United States)
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1579978
Report Number(s):
NREL/JA--2700-75366
Journal Information:
Green Chemistry, Journal Name: Green Chemistry Journal Issue: 2 Vol. 22; ISSN GRCHFJ; ISSN 1463-9262
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United Kingdom
Language:
English

References (29)

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Multifunctional Cellulolytic Enzymes Outperform Processive Fungal Cellulases for Coproduction of Nanocellulose and Biofuels journal February 2017
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Crystal Structure of Thermostable Family 5 Endocellulase E1 from Acidothermus cellulolyticus in Complex with Cellotetraose † , ‡ journal January 1996
The Mechanism of Cellulose Hydrolysis by a Two-Step, Retaining Cellobiohydrolase Elucidated by Structural and Transition Path Sampling Studies journal December 2013
Effects of Sulfate Groups on the Adsorption and Activity of Cellulases on Cellulose Substrates journal February 2013
Enzymatic Kinetics of Cellulose Hydrolysis:  A QCM-D Study journal April 2008
Enzymatic Hydrolysis of Native Cellulose Nanofibrils and Other Cellulose Model Films: Effect of Surface Structure journal October 2008
Non–Hydrolytic Disruption of Cellulose Fibres by the Binding Domain of a Bacterial Cellulase journal November 1991
Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca journal October 1997
Engineering enhanced cellobiohydrolase activity journal March 2018
The Multi Domain Caldicellulosiruptor bescii CelA Cellulase Excels at the Hydrolysis of Crystalline Cellulose journal August 2017
Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass journal October 2016
Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei journal August 1998
Glycosylated linkers in multimodular lignocellulose-degrading enzymes dynamically bind to cellulose journal August 2013
Distinct roles of N- and O-glycans in cellulase activity and stability journal December 2017
Degradation of Cellulose Substrates by Cellulosome Chimeras: SUBSTRATE TARGETING journal October 2002
SWISS-MODEL: homology modelling of protein structures and complexes journal May 2018
Cellulose crystallinity - a key predictor of the enzymatic hydrolysis rate: Cellulose crystallinity journal February 2010
Revealing Nature's Cellulase Diversity The Digestion Mechanism of Caldicellulosiruptor bescii CelA journal December 2013
Global View of the Clostridium thermocellum Cellulosome Revealed by Quantitative Proteomic Analysis journal July 2007
Cellulose crystallinity index: measurement techniques and their impact on interpreting cellulase performance journal January 2010
A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates journal January 2013
A constitutive expression system for glycosyl hydrolase family 7 cellobiohydrolases in Hypocrea jecorina journal March 2015
Techno-economic analysis of the deacetylation and disk refining process: characterizing the effect of refining energy and enzyme usage on minimum sugar selling price and minimum ethanol selling price journal October 2015
A versatile 2A peptide-based bicistronic protein expressing platform for the industrial cellulase producing fungus, Trichoderma reesei journal February 2017
Comparative insights into the saccharification potentials of a relatively unexplored but robust Penicillium funiculosum glycoside hydrolase 7 cellobiohydrolase journal March 2017
High activity CAZyme cassette for improving biomass degradation in thermophiles journal February 2018
Catalytically Enhanced Endocellulase Cel5A from Acidothermus cellulolyticus journal January 2005

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