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Title: Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination

Abstract

Accurate medical recordkeeping is a major challenge in many low-resource settings where well-maintained centralized databases do not exist, contributing to 1.5 million vaccine-preventable deaths annually. Here, we present an approach to encode medical history on a patient using the spatial distribution of biocompatible, near-infrared quantum dots (NIR QDs) in the dermis. QDs are invisible to the naked eye yet detectable when exposed to NIR light. QDs with a copper indium selenide core and aluminum-doped zinc sulfide shell were tuned to emit in the NIR spectrum by controlling stoichiometry and shelling time. The formulation showing the greatest resistance to photobleaching after simulated sunlight exposure (5-year equivalence) through pigmented human skin was encapsulated in microparticles for use in vivo. In parallel, microneedle geometry was optimized in silico and validated ex vivo using porcine and synthetic human skin. QD-containing microparticles were then embedded in dissolvable microneedles and administered to rats with or without a vaccine. Longitudinal in vivo imaging using a smartphone adapted to detect NIR light demonstrated that microneedle-delivered QD patterns remained bright and could be accurately identified using a machine learning algorithm 9 months after application. In addition, codelivery with inactivated poliovirus vaccine produced neutralizing antibody titers above the threshold consideredmore » protective. These findings suggest that intradermal QDs can be used to reliably encode information and can be delivered with a vaccine, which may be particularly valuable in the developing world and open up new avenues for decentralized data storage and biosensing.« less

Authors:
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Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1579790
Grant/Contract Number:  
SC0001088
Resource Type:
Published Article
Journal Name:
Science Translational Medicine
Additional Journal Information:
Journal Name: Science Translational Medicine Journal Volume: 11 Journal Issue: 523; Journal ID: ISSN 1946-6234
Publisher:
American Association for the Advancement of Science (AAAS)
Country of Publication:
United States
Language:
English

Citation Formats

McHugh, Kevin J., Jing, Lihong, Severt, Sean Y., Cruz, Mache, Sarmadi, Morteza, Jayawardena, Hapuarachchige Surangi N., Perkinson, Collin F., Larusson, Fridrik, Rose, Sviatlana, Tomasic, Stephanie, Graf, Tyler, Tzeng, Stephany Y., Sugarman, James L., Vlasic, Daniel, Peters, Matthew, Peterson, Nels, Wood, Lowell, Tang, Wen, Yeom, Jihyeon, Collins, Joe, Welkhoff, Philip A., Karchin, Ari, Tse, Megan, Gao, Mingyuan, Bawendi, Moungi G., Langer, Robert, and Jaklenec, Ana. Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination. United States: N. p., 2019. Web. doi:10.1126/scitranslmed.aay7162.
McHugh, Kevin J., Jing, Lihong, Severt, Sean Y., Cruz, Mache, Sarmadi, Morteza, Jayawardena, Hapuarachchige Surangi N., Perkinson, Collin F., Larusson, Fridrik, Rose, Sviatlana, Tomasic, Stephanie, Graf, Tyler, Tzeng, Stephany Y., Sugarman, James L., Vlasic, Daniel, Peters, Matthew, Peterson, Nels, Wood, Lowell, Tang, Wen, Yeom, Jihyeon, Collins, Joe, Welkhoff, Philip A., Karchin, Ari, Tse, Megan, Gao, Mingyuan, Bawendi, Moungi G., Langer, Robert, & Jaklenec, Ana. Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination. United States. doi:10.1126/scitranslmed.aay7162.
McHugh, Kevin J., Jing, Lihong, Severt, Sean Y., Cruz, Mache, Sarmadi, Morteza, Jayawardena, Hapuarachchige Surangi N., Perkinson, Collin F., Larusson, Fridrik, Rose, Sviatlana, Tomasic, Stephanie, Graf, Tyler, Tzeng, Stephany Y., Sugarman, James L., Vlasic, Daniel, Peters, Matthew, Peterson, Nels, Wood, Lowell, Tang, Wen, Yeom, Jihyeon, Collins, Joe, Welkhoff, Philip A., Karchin, Ari, Tse, Megan, Gao, Mingyuan, Bawendi, Moungi G., Langer, Robert, and Jaklenec, Ana. Wed . "Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination". United States. doi:10.1126/scitranslmed.aay7162.
@article{osti_1579790,
title = {Biocompatible near-infrared quantum dots delivered to the skin by microneedle patches record vaccination},
author = {McHugh, Kevin J. and Jing, Lihong and Severt, Sean Y. and Cruz, Mache and Sarmadi, Morteza and Jayawardena, Hapuarachchige Surangi N. and Perkinson, Collin F. and Larusson, Fridrik and Rose, Sviatlana and Tomasic, Stephanie and Graf, Tyler and Tzeng, Stephany Y. and Sugarman, James L. and Vlasic, Daniel and Peters, Matthew and Peterson, Nels and Wood, Lowell and Tang, Wen and Yeom, Jihyeon and Collins, Joe and Welkhoff, Philip A. and Karchin, Ari and Tse, Megan and Gao, Mingyuan and Bawendi, Moungi G. and Langer, Robert and Jaklenec, Ana},
abstractNote = {Accurate medical recordkeeping is a major challenge in many low-resource settings where well-maintained centralized databases do not exist, contributing to 1.5 million vaccine-preventable deaths annually. Here, we present an approach to encode medical history on a patient using the spatial distribution of biocompatible, near-infrared quantum dots (NIR QDs) in the dermis. QDs are invisible to the naked eye yet detectable when exposed to NIR light. QDs with a copper indium selenide core and aluminum-doped zinc sulfide shell were tuned to emit in the NIR spectrum by controlling stoichiometry and shelling time. The formulation showing the greatest resistance to photobleaching after simulated sunlight exposure (5-year equivalence) through pigmented human skin was encapsulated in microparticles for use in vivo. In parallel, microneedle geometry was optimized in silico and validated ex vivo using porcine and synthetic human skin. QD-containing microparticles were then embedded in dissolvable microneedles and administered to rats with or without a vaccine. Longitudinal in vivo imaging using a smartphone adapted to detect NIR light demonstrated that microneedle-delivered QD patterns remained bright and could be accurately identified using a machine learning algorithm 9 months after application. In addition, codelivery with inactivated poliovirus vaccine produced neutralizing antibody titers above the threshold considered protective. These findings suggest that intradermal QDs can be used to reliably encode information and can be delivered with a vaccine, which may be particularly valuable in the developing world and open up new avenues for decentralized data storage and biosensing.},
doi = {10.1126/scitranslmed.aay7162},
journal = {Science Translational Medicine},
number = 523,
volume = 11,
place = {United States},
year = {2019},
month = {12}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/scitranslmed.aay7162

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