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Title: Prostaglandin E 2 receptor EP4 regulates cell migration through Orai1

Journal Article · · Cancer Science
DOI: https://doi.org/10.1111/cas.14247 · OSTI ID:1579781
 [1]; ORCiD logo [2];  [1];  [2];  [2];  [2];  [2];  [3];  [4];  [4];  [2]
  1. Cardiovascular Research Institute Yokohama City University Graduate School of Medicine Yokohama Japan, Department of Oral and Maxillofacial Surgery Yokohama City University Graduate School of Medicine Yokohama Japan
  2. Cardiovascular Research Institute Yokohama City University Graduate School of Medicine Yokohama Japan
  3. Cardiovascular Research Institute Yokohama City University Graduate School of Medicine Yokohama Japan, Department of Physiology Tokyo Medical University Graduate School of Medicine Tokyo Japan
  4. Department of Oral and Maxillofacial Surgery Yokohama City University Graduate School of Medicine Yokohama Japan
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Abstract The EP4 prostanoid receptors are one of four receptor subtypes for prostaglandin E 2 (PGE 2 ). Therefore, EP4 may play an important role in cancer progression. However, little information is available regarding their function per se, including migration and the cellular signaling pathway of EP4 in oral cancer. First, we found that mRNA and protein expression of EP4 was abundantly expressed in human‐derived tongue squamous cell carcinoma cell lines HSC‐3 and OSC‐19. The EP4 agonist (ONO‐AE1‐437) significantly promoted cell migration in HSC‐3 cells. In contrast, knockdown of EP4 reduced cell migration. Furthermore, we confirmed that knockdown of EP4 suppressed metastasis of oral cancer cells in the lungs of mice in vivo. Therefore, we focused on the mechanism of migration/metastasis in EP4 signaling. Interestingly, EP4 agonist significantly induced intracellular Ca 2+ elevation not in only oral cancer cells but also in other cells, including normal cells. Furthermore, we found that EP4 activated PI3K and induced Ca 2+ influx through Orai1 without activation of store depletion and stromal interaction molecule 1 (STIM1). Immunoprecipitation showed that EP4 formed complexes with Orai1 and TRPC1, but not with STIM. Moreover, the EP4 agonist ONO‐AE1‐437 phosphorylated ERK and activated MMP‐2 and MMP‐9. Knockdown of Orai1 negated EP4 agonist‐induced ERK phosphorylation. Taken together, our data suggested that EP4 activated PI3K and then induced Ca 2+ influx from the extracellular space through Orai1, resulting in ERK phosphorylation and promoting cell migration. Migration is regulated by EP4/PI3K/Orai1 signaling in oral cancer.

Sponsoring Organization:
USDOE
OSTI ID:
1579781
Journal Information:
Cancer Science, Journal Name: Cancer Science Journal Issue: 1 Vol. 111; ISSN 1347-9032
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
Japan
Language:
English

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