A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor

doi:10.1002/cbic.201900654

This content will become publicly available on December 15, 2020

Title: A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor

Authors:

Newcomb, Megan P. ^[1]; Lee, Chi Chung ^[2]; Tanifuji, Kazuki ^[2]; Jasniewski, Andrew J. ^[2]; Liedtke, Jasper ^[2];

^[3]; Hu, Yilin ^[2]

Department of ChemistryUniversity of California, Irvine Natural Sciences II Irvine CA 92697-2025 USA
Department of Molecular Biology and BiochemistryUniversity of California, Irvine 3205 McGaugh Hall Irvine CA 92697-3900 USA
Department of ChemistryUniversity of California, Irvine Natural Sciences II Irvine CA 92697-2025 USA, Department of Molecular Biology and BiochemistryUniversity of California, Irvine 3205 McGaugh Hall Irvine CA 92697-3900 USA

Publication Date:: 2019-12-16

Sponsoring Org.:: USDOE

OSTI Identifier:: 1579471

Grant/Contract Number:: SC0016510; SC0014470

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: ChemBioChem: a European journal of chemical biology

Additional Journal Information:: Journal Name: ChemBioChem: a European journal of chemical biology; Journal ID: ISSN 1439-4227

Publisher:: Wiley Blackwell (John Wiley & Sons)

Country of Publication:: France

Language:: English

Citation Formats


                    Newcomb, Megan P., Lee, Chi Chung, Tanifuji, Kazuki, Jasniewski, Andrew J., Liedtke, Jasper, Ribbe, Markus W., and Hu, Yilin. A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor.  France: N. p., 2019. 
        Web.  doi:10.1002/cbic.201900654.

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                    Newcomb, Megan P., Lee, Chi Chung, Tanifuji, Kazuki, Jasniewski, Andrew J., Liedtke, Jasper, Ribbe, Markus W., & Hu, Yilin. A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor.  France.  doi:10.1002/cbic.201900654.

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                    Newcomb, Megan P., Lee, Chi Chung, Tanifuji, Kazuki, Jasniewski, Andrew J., Liedtke, Jasper, Ribbe, Markus W., and Hu, Yilin. Mon .  
        "A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor".  France.  doi:10.1002/cbic.201900654.

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@article{osti_1579471,

  title        = {A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor},

  author       = {Newcomb, Megan P. and Lee, Chi Chung and Tanifuji, Kazuki and Jasniewski, Andrew J. and Liedtke, Jasper and Ribbe, Markus W. and Hu, Yilin},

  abstractNote = {},

  doi          = {10.1002/cbic.201900654},

  journal      = {ChemBioChem: a European journal of chemical biology},

  number       = ,

  volume       = ,

  place        = {France},

  year         = {2019},

  month        = {12}

}

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DOI: 10.1002/cbic.201900654

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Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.
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This content will become publicly available on December 15, 2020

Title: A V‐Nitrogenase Variant Containing a Citrate‐Substituted Cofactor

Citation Formats

Characterization of Azotobacter vinelandii nifZ Deletion Strains : INDICATION OF STEPWISE MoFe PROTEIN ASSEMBLY journal, October 2004

Nitrogenase reactivity with P-cluster variants journal, September 2005

Negative cooperativity in the nitrogenase Fe protein electron delivery cycle journal, October 2016

Tracing the Hydrogen Source of Hydrocarbons Formed by Vanadium Nitrogenase journal, April 2011

Investigation of CO bound to inhibited forms of nitrogenase MoFe protein by 13C ENDOR journal, August 1995

nifV-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase. journal, September 1986

The controlled relay of multiple protons required at the active site of nitrogenase journal, January 2012

Mechanism of Nitrogen Fixation by Nitrogenase: The Next Stage journal, January 2014

Nitrogenase of Klebsiella pneumoniae nifV mutants. Investigation of the novel carbon monoxide-sensitivity of hydrogen evolution by the mutant enzyme journal, June 1983

Identification of the V factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrate journal, October 1987

Biosynthesis of the Metalloclusters of Nitrogenases journal, June 2016

Tracing the Hydrogen Source of Hydrocarbons Formed by Vanadium Nitrogenase journal, April 2011

Structure−Function Relationships of Alternative Nitrogenases journal, January 1996

Structural basis of biological nitrogen fixation journal, April 2005

Isolation of Two Forms of the Nitrogenase VFe Protein from Azotobacter vinelandii † journal, January 1996

A Comparative Analysis of the CO-Reducing Activities of MoFe Proteins Containing Mo- and V-Nitrogenase Cofactors journal, February 2018

Citrate substitutes for homocitrate in nitrogenase of a nifV mutant of Klebsiella pneumoniae journal, September 1990

Unique features of the nitrogenase VFe protein from Azotobacter vinelandii journal, May 2009

Crystallographic Analysis of the MoFe Protein of Nitrogenase from a nifV Mutant of Klebsiella pneumoniae Identifies Citrate as a Ligand to the Molybdenum of Iron Molybdenum Cofactor (FeMoco) journal, July 2002

Large-scale purification of high activity Azotobacter vinelandii nitrogenase journal, July 1980

Extending the Carbon Chain: Hydrocarbon Formation Catalyzed by Vanadium/Molybdenum Nitrogenases journal, August 2011

Mechanism of Molybdenum Nitrogenase journal, January 1996

Competitive Substrate and Inhibitor Interactions at the Physiologically Relevant Active Site of Nitrogenase journal, August 2000

Characterization of an M-Cluster-Substituted Nitrogenase VFe Protein journal, March 2018

Requirement of nifV gene for production of wild-type nitrogenase enzyme in Klebsiella pneumoniae journal, August 1981

CO Binding to the FeMo Cofactor of CO-Inhibited Nitrogenase: 13 CO and 1 H Q-Band ENDOR Investigation journal, October 1997

Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase. journal, September 1997

Molecular Insights into Nitrogenase FeMoco Insertion journal, August 2006

The structure of vanadium nitrogenase reveals an unusual bridging ligand journal, July 2017

Catalytic Reduction of Hydrazine to Ammonia with MoFe 3 S 4 −Polycarboxylate Clusters. Possible Relevance Regarding the Function of the Molybdenum-Coordinated Homocitrate in Nitrogenase journal, January 1996

Nitrogenase and homologs journal, December 2014

Reduction of C 1 Substrates to Hydrocarbons by the Homometallic Precursor and Synthetic Mimic of the Nitrogenase Cofactor journal, January 2017

Vanadium Nitrogenase Reduces CO journal, August 2010

A comprehensive mechanism for the Fischer-Tropsch synthesis journal, October 1981

Time-Resolved Binding of Carbon Monoxide to Nitrogenase Monitored by Stopped-Flow Infrared Spectroscopy journal, July 1997

Characterization of Azotobacter vinelandii nifZ Deletion Strains : INDICATION OF STEPWISE MoFe PROTEIN ASSEMBLY
journal, October 2004

Nitrogenase reactivity with P-cluster variants
journal, September 2005

Negative cooperativity in the nitrogenase Fe protein electron delivery cycle
journal, October 2016

Tracing the Hydrogen Source of Hydrocarbons Formed by Vanadium Nitrogenase
journal, April 2011

Investigation of CO bound to inhibited forms of nitrogenase MoFe protein by 13C ENDOR
journal, August 1995

nifV-dependent, low-molecular-weight factor required for in vitro synthesis of iron-molybdenum cofactor of nitrogenase.
journal, September 1986

The controlled relay of multiple protons required at the active site of nitrogenase
journal, January 2012

Mechanism of Nitrogen Fixation by Nitrogenase: The Next Stage
journal, January 2014

Nitrogenase of Klebsiella pneumoniae nifV mutants. Investigation of the novel carbon monoxide-sensitivity of hydrogen evolution by the mutant enzyme
journal, June 1983

Identification of the V factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrate
journal, October 1987

Biosynthesis of the Metalloclusters of Nitrogenases
journal, June 2016

Tracing the Hydrogen Source of Hydrocarbons Formed by Vanadium Nitrogenase
journal, April 2011

Structure−Function Relationships of Alternative Nitrogenases
journal, January 1996

Structural basis of biological nitrogen fixation
journal, April 2005

Isolation of Two Forms of the Nitrogenase VFe Protein from Azotobacter vinelandii ^†
journal, January 1996

A Comparative Analysis of the CO-Reducing Activities of MoFe Proteins Containing Mo- and V-Nitrogenase Cofactors
journal, February 2018

Citrate substitutes for homocitrate in nitrogenase of a nifV mutant of Klebsiella pneumoniae
journal, September 1990

Unique features of the nitrogenase VFe protein from Azotobacter vinelandii
journal, May 2009

Crystallographic Analysis of the MoFe Protein of Nitrogenase from a nifV Mutant of Klebsiella pneumoniae Identifies Citrate as a Ligand to the Molybdenum of Iron Molybdenum Cofactor (FeMoco)
journal, July 2002

Large-scale purification of high activity Azotobacter vinelandii nitrogenase
journal, July 1980

Extending the Carbon Chain: Hydrocarbon Formation Catalyzed by Vanadium/Molybdenum Nitrogenases
journal, August 2011

Mechanism of Molybdenum Nitrogenase
journal, January 1996

Competitive Substrate and Inhibitor Interactions at the Physiologically Relevant Active Site of Nitrogenase
journal, August 2000

Characterization of an M-Cluster-Substituted Nitrogenase VFe Protein
journal, March 2018

Requirement of nifV gene for production of wild-type nitrogenase enzyme in Klebsiella pneumoniae
journal, August 1981

CO Binding to the FeMo Cofactor of CO-Inhibited Nitrogenase: ¹³ CO and ¹ H Q-Band ENDOR Investigation
journal, October 1997

Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase.
journal, September 1997

Molecular Insights into Nitrogenase FeMoco Insertion
journal, August 2006

The structure of vanadium nitrogenase reveals an unusual bridging ligand
journal, July 2017

Catalytic Reduction of Hydrazine to Ammonia with MoFe ₃ S ₄ −Polycarboxylate Clusters. Possible Relevance Regarding the Function of the Molybdenum-Coordinated Homocitrate in Nitrogenase
journal, January 1996

Nitrogenase and homologs
journal, December 2014

Reduction of C ₁ Substrates to Hydrocarbons by the Homometallic Precursor and Synthetic Mimic of the Nitrogenase Cofactor
journal, January 2017

Vanadium Nitrogenase Reduces CO
journal, August 2010

A comprehensive mechanism for the Fischer-Tropsch synthesis
journal, October 1981

Time-Resolved Binding of Carbon Monoxide to Nitrogenase Monitored by Stopped-Flow Infrared Spectroscopy
journal, July 1997