Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis

doi:10.1016/j.jfda.2019.12.001

Title: Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis

Full Record
Other Related Research

Authors:: Xia, Qingsu; He, Xiaobo; Shi, Qiang; Lin, Ge; Fu, Peter P.

Publication Date:: 2020-01-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 1579432

Resource Type:: Published Article

Journal Name:: Journal of Food and Drug Analysis

Additional Journal Information:: Journal Name: Journal of Food and Drug Analysis Journal Volume: 28 Journal Issue: 1; Journal ID: ISSN 1021-9498

Publisher:: Elsevier

Country of Publication:: Country unknown/Code not available

Language:: English

Citation Formats


                    Xia, Qingsu, He, Xiaobo, Shi, Qiang, Lin, Ge, and Fu, Peter P. Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis.  Country unknown/Code not available: N. p., 2020. 
        Web.  doi:10.1016/j.jfda.2019.12.001.

Copy to clipboard


                    Xia, Qingsu, He, Xiaobo, Shi, Qiang, Lin, Ge, & Fu, Peter P. Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis.  Country unknown/Code not available.  doi:10.1016/j.jfda.2019.12.001.

Copy to clipboard


                    Xia, Qingsu, He, Xiaobo, Shi, Qiang, Lin, Ge, and Fu, Peter P. Wed .  
        "Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis".  Country unknown/Code not available.  doi:10.1016/j.jfda.2019.12.001.

Copy to clipboard


                    
@article{osti_1579432,

  title        = {Quantitation of DNA reactive pyrrolic metabolites of senecionine – A carcinogenic pyrrolizidine alkaloid by LC/MS/MS analysis},

  author       = {Xia, Qingsu and He, Xiaobo and Shi, Qiang and Lin, Ge and Fu, Peter P.},

  abstractNote = {},

  doi          = {10.1016/j.jfda.2019.12.001},

  journal      = {Journal of Food and Drug Analysis},

  number       = 1,

  volume       = 28,

  place        = {Country unknown/Code not available},

  year         = {2020},

  month        = {1}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Publisher's Version of Record
DOI: 10.1016/j.jfda.2019.12.001

Other availability

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

7- N -Acetylcysteine-pyrrole conjugate—A potent DNA reactive metabolite of pyrrolizidine alkaloids

Journal Article He, Xiaobo ; Ma, Liang ; Xia, Qingsu ; ... - Journal of Food and Drug Analysis
Cited by 1
DOI: 10.1016/j.jfda.2016.08.001
Characterization of hepatic DNA damage induced in rats by the pyrrolizidine alkaloid monocrotaline

Journal Article Petry, T W ; Bowden, G T ; Huxtable, R J ; ... - Cancer Res.; (United States)

Hepatic DNA damage induced by the pyrrolizidine alkaloid monocrotaline was evaluated following i.p. administration to adult male Sprague-Dawley rats. Animals were treated with various doses ranging upward from 5 mg/kg, and hepatic nuclei were isolated 4 hr later. Hepatic nuclei were used as the DNA source in all experiments. DNA damage was characterized by the alkaline elution technique. A mixture of DNA-DNA interstrand cross-links and DNA-protein cross-links was induced. Following an injection of monocrotaline, 30 mg/kg i.p., DNA-DNA interstrand cross-linking reached a maximum within 12 hr or less and thereafter decreased over a protracted period of time. By 96 hrmore »« less
Comparative reactivities of diolepoxide metabolites of carcinogenic hydrocarbons with phiX174 viral DNA

Journal Article Hsu, W T ; Lin, E J ; Fu, P P ; ... - Biochem. Biophys. Res. Commun.; (United States)

The phiX174 DNA assay system developed earlier is utilized to determine the comparative reactivities with nucleic acid of the diolepoxide metabolites of a series of polycyclic aromatic hydrocarbons varying in carcinogenic potency. The infectious phiX174 viral DNA is exposed to the hydrocarbon derivative for 10 min, then infectivity of the treated DNA is assayed by incubation with E. coli spheroplasts, counting plaque formation on agar plates. The bay region diolepoxides of benzo(a)-pyrene, chrysene, and dibenz(a,h)anthracene, implicated as the ultimately active carcinogenic metabolites of the parent hydrocarbons, exhibit potent viral inhibitory activity. On the other hand no correlation is evident betweenmore »« less
DOI: 10.1016/0006-291X(79)91723-6
Trapping of DNA-reactive metabolites of therapeutic or carcinogenic agents by /sup 14/C-labeled synthetic polynucleotides

Journal Article Mehta, J R ; Ludlum, D B - Cancer Res.; (United States)

Many substances which do not react with DNA directly are metabolized into important DNA-modifying intermediates. We have devised a method for trapping these intermediates with /sup 14/C-labeled nucleosides contained in a synthetic polynucleotide. The polynucleotide structure protects the labeled nucleoside from metabolism; thus, it is unaltered when the polymer is incubated with a drug-metabolizing system. However, when the polymer is incubated with this system and a compound which can be metabolized into a reactive species, these intermediates are trapped by the /sup 14/C-labeled nucleoside and subsequently are detected as new peaks of radioactivity in a digest of the labeled polynucleotide.more »« less
Sources of Technical Variability in Quantitative LC-MS Proteomics: Human Brain Tissue Sample Analysis.

Journal Article Piehowski, Paul D. ; Petyuk, Vladislav A. ; Orton, Daniel J. ; ... - Journal of Proteome Research, 12(5):2128-2137

To design a robust quantitative proteomics study, an understanding of both the inherent heterogeneity of the biological samples being studied as well as the technical variability of the proteomics methods and platform is needed. Additionally, accurately identifying the technical steps associated with the largest variability would provide valuable information for the improvement and design of future processing pipelines. We present an experimental strategy that allows for a detailed examination of the variability of the quantitative LC-MS proteomics measurements. By replicating analyses at different stages of processing, various technical components can be estimated and their individual contribution to technical variability canmore »« less
DOI: 10.1021/pr301146m

Similar Records