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Title: Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems

Abstract

The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system. This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors.

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. University of Wisconsin–Madison, Laboratory for Optical and Computational Instrumentation, Madison,
  2. University of Wisconsin–Madison, Mechanical Engineering Department, Madison, Wisconsin
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE; Morgridge Institute for Research; Laboratory for Optical and Computational Instrumentation; National Institutes of Health (NIH)
OSTI Identifier:
1579396
Alternate Identifier(s):
OSTI ID: 1817175
Grant/Contract Number:  
0000238219; R01 CA185251
Resource Type:
Published Article
Journal Name:
Journal of Biomedical Optics
Additional Journal Information:
Journal Name: Journal of Biomedical Optics Journal Volume: 25 Journal Issue: 01; Journal ID: ISSN 1083-3668
Publisher:
SPIE-Intl Soc Optical Eng
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION; hyperspectral imaging; multiphoton microscopy; fiber-based dispersion; time-correlated single-photon counting; fluorescence lifetime imaging; fluorescence lifetime

Citation Formats

Sagar, Md Abdul Kader, Dai, Bing, Chacko, Jenu V., Weber, Joshua J., Velten, Andreas, Sanders, Scott T., White, John G., and Eliceiri, Kevin W. Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems. United States: N. p., 2019. Web. doi:10.1117/1.JBO.25.1.014506.
Sagar, Md Abdul Kader, Dai, Bing, Chacko, Jenu V., Weber, Joshua J., Velten, Andreas, Sanders, Scott T., White, John G., & Eliceiri, Kevin W. Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems. United States. https://doi.org/10.1117/1.JBO.25.1.014506
Sagar, Md Abdul Kader, Dai, Bing, Chacko, Jenu V., Weber, Joshua J., Velten, Andreas, Sanders, Scott T., White, John G., and Eliceiri, Kevin W. Thu . "Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems". United States. https://doi.org/10.1117/1.JBO.25.1.014506.
@article{osti_1579396,
title = {Optical fiber-based dispersion for spectral discrimination in fluorescence lifetime imaging systems},
author = {Sagar, Md Abdul Kader and Dai, Bing and Chacko, Jenu V. and Weber, Joshua J. and Velten, Andreas and Sanders, Scott T. and White, John G. and Eliceiri, Kevin W.},
abstractNote = {The excited state lifetime of a fluorophore together with its fluorescence emission spectrum provide information that can yield valuable insights into the nature of a fluorophore and its microenvironment. However, it is difficult to obtain both channels of information in a conventional scheme as detectors are typically configured either for spectral or lifetime detection. We present a fiber-based method to obtain spectral information from a multiphoton fluorescence lifetime imaging (FLIM) system. This is made possible using the time delay introduced in the fluorescence emission path by a dispersive optical fiber coupled to a detector operating in time-correlated single-photon counting mode. This add-on spectral implementation requires only a few simple modifications to any existing FLIM system and is considerably more cost-efficient compared to currently available spectral detectors.},
doi = {10.1117/1.JBO.25.1.014506},
journal = {Journal of Biomedical Optics},
number = 01,
volume = 25,
place = {United States},
year = {2019},
month = {12}
}