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Title: Assembly and seasonality of core phyllosphere microbiota on perennial biofuel crops

Abstract

Perennial grasses are promising feedstocks for biofuel production, with potential for leveraging their native microbiomes to increase their productivity and resilience to environmental stress. Here, we characterize the 16S rRNA gene diversity and seasonal assembly of bacterial and archaeal microbiomes of two perennial cellulosic feedstocks, switchgrass ( Panicum virgatum L.) and miscanthus ( Miscanthus x giganteus). We sample leaves and soil every three weeks from pre-emergence through senescence for two consecutive switchgrass growing seasons and one miscanthus season, and identify core leaf taxa based on occupancy. Virtually all leaf taxa are also detected in soil; source-sink modeling shows non-random, ecological filtering by the leaf, suggesting that soil is an important reservoir of phyllosphere diversity. Core leaf taxa include early, mid, and late season groups that were consistent across years and crops. This consistency in leaf microbiome dynamics and core members is promising for microbiome manipulation or management to support crop production.

Authors:
 [1];  [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]
  1. Michigan State Univ., East Lansing, MI (United States)
Publication Date:
Research Org.:
Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1579346
Grant/Contract Number:  
SC0018409; FC02–07ER64494; AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Grady, Keara L., Sorensen, Jackson W., Stopnisek, Nejc, Guittar, John, and Shade, Ashley. Assembly and seasonality of core phyllosphere microbiota on perennial biofuel crops. United States: N. p., 2019. Web. doi:10.1038/s41467-019-11974-4.
Grady, Keara L., Sorensen, Jackson W., Stopnisek, Nejc, Guittar, John, & Shade, Ashley. Assembly and seasonality of core phyllosphere microbiota on perennial biofuel crops. United States. doi:10.1038/s41467-019-11974-4.
Grady, Keara L., Sorensen, Jackson W., Stopnisek, Nejc, Guittar, John, and Shade, Ashley. Thu . "Assembly and seasonality of core phyllosphere microbiota on perennial biofuel crops". United States. doi:10.1038/s41467-019-11974-4. https://www.osti.gov/servlets/purl/1579346.
@article{osti_1579346,
title = {Assembly and seasonality of core phyllosphere microbiota on perennial biofuel crops},
author = {Grady, Keara L. and Sorensen, Jackson W. and Stopnisek, Nejc and Guittar, John and Shade, Ashley},
abstractNote = {Perennial grasses are promising feedstocks for biofuel production, with potential for leveraging their native microbiomes to increase their productivity and resilience to environmental stress. Here, we characterize the 16S rRNA gene diversity and seasonal assembly of bacterial and archaeal microbiomes of two perennial cellulosic feedstocks, switchgrass (Panicum virgatum L.) and miscanthus (Miscanthus x giganteus). We sample leaves and soil every three weeks from pre-emergence through senescence for two consecutive switchgrass growing seasons and one miscanthus season, and identify core leaf taxa based on occupancy. Virtually all leaf taxa are also detected in soil; source-sink modeling shows non-random, ecological filtering by the leaf, suggesting that soil is an important reservoir of phyllosphere diversity. Core leaf taxa include early, mid, and late season groups that were consistent across years and crops. This consistency in leaf microbiome dynamics and core members is promising for microbiome manipulation or management to support crop production.},
doi = {10.1038/s41467-019-11974-4},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {9}
}

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