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Title: The elaborate route for UDP-arabinose delivery into the Golgi of plants

Abstract

In plants, L-arabinose (Ara) is a key component of cell wall polymers, glycoproteins, as well as flavonoids, and signaling peptides. Whereas the majority of Ara found in plant glycans occurs as a furanose ring (Ara f ), the activated precursor has a pyranose ring configuration (UDP-Ara p ). The biosynthesis of UDP-Ara p mainly occurs via the epimerization of UDP-xylose (UDP-Xyl) in the Golgi lumen. Given that the predominant Ara form found in plants is Ara f , UDP-Ara p must exit the Golgi to be interconverted into UDP-Ara f by UDP-Ara mutases that are located outside on the cytosolic surface of the Golgi. Subsequently, UDP-Ara f must be transported back into the lumen. This step is vital because glycosyltransferases, the enzymes mediating the glycosylation reactions, are located within the Golgi lumen, and UDP-Ara p , synthesized within the Golgi, is not their preferred substrate. Thus, the transport of UDP-Ara f into the Golgi is a prerequisite. Although this step is critical for cell wall biosynthesis and the glycosylation of proteins and signaling peptides, the identification of these transporters has remained elusive. In this study, we present data demonstrating the identification and characterization of a family of Golgi-localized UDP-Ara fmore » transporters in Arabidopsis . The application of a proteoliposome-based transport assay revealed that four members of the nucleotide sugar transporter (NST) family can efficiently transport UDP-Ara f in vitro. Subsequent analysis of mutant lines affected in the function of these NSTs confirmed their role as UDP-Ara f transporters in vivo.« less

Authors:
; ; ; ; ; ; ; ORCiD logo; ; ORCiD logo;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1579294
Alternate Identifier(s):
OSTI ID: 1393224
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 114 Journal Issue: 16; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; golgi apparatus; nucleotide sugars; membrane transport; arabinose

Citation Formats

Rautengarten, Carsten, Birdseye, Devon, Pattathil, Sivakumar, McFarlane, Heather E., Saez-Aguayo, Susana, Orellana, Ariel, Persson, Staffan, Hahn, Michael G., Scheller, Henrik V., Heazlewood, Joshua L., and Ebert, Berit. The elaborate route for UDP-arabinose delivery into the Golgi of plants. United States: N. p., 2017. Web. doi:10.1073/pnas.1701894114.
Rautengarten, Carsten, Birdseye, Devon, Pattathil, Sivakumar, McFarlane, Heather E., Saez-Aguayo, Susana, Orellana, Ariel, Persson, Staffan, Hahn, Michael G., Scheller, Henrik V., Heazlewood, Joshua L., & Ebert, Berit. The elaborate route for UDP-arabinose delivery into the Golgi of plants. United States. doi:10.1073/pnas.1701894114.
Rautengarten, Carsten, Birdseye, Devon, Pattathil, Sivakumar, McFarlane, Heather E., Saez-Aguayo, Susana, Orellana, Ariel, Persson, Staffan, Hahn, Michael G., Scheller, Henrik V., Heazlewood, Joshua L., and Ebert, Berit. Mon . "The elaborate route for UDP-arabinose delivery into the Golgi of plants". United States. doi:10.1073/pnas.1701894114.
@article{osti_1579294,
title = {The elaborate route for UDP-arabinose delivery into the Golgi of plants},
author = {Rautengarten, Carsten and Birdseye, Devon and Pattathil, Sivakumar and McFarlane, Heather E. and Saez-Aguayo, Susana and Orellana, Ariel and Persson, Staffan and Hahn, Michael G. and Scheller, Henrik V. and Heazlewood, Joshua L. and Ebert, Berit},
abstractNote = {In plants, L-arabinose (Ara) is a key component of cell wall polymers, glycoproteins, as well as flavonoids, and signaling peptides. Whereas the majority of Ara found in plant glycans occurs as a furanose ring (Ara f ), the activated precursor has a pyranose ring configuration (UDP-Ara p ). The biosynthesis of UDP-Ara p mainly occurs via the epimerization of UDP-xylose (UDP-Xyl) in the Golgi lumen. Given that the predominant Ara form found in plants is Ara f , UDP-Ara p must exit the Golgi to be interconverted into UDP-Ara f by UDP-Ara mutases that are located outside on the cytosolic surface of the Golgi. Subsequently, UDP-Ara f must be transported back into the lumen. This step is vital because glycosyltransferases, the enzymes mediating the glycosylation reactions, are located within the Golgi lumen, and UDP-Ara p , synthesized within the Golgi, is not their preferred substrate. Thus, the transport of UDP-Ara f into the Golgi is a prerequisite. Although this step is critical for cell wall biosynthesis and the glycosylation of proteins and signaling peptides, the identification of these transporters has remained elusive. In this study, we present data demonstrating the identification and characterization of a family of Golgi-localized UDP-Ara f transporters in Arabidopsis . The application of a proteoliposome-based transport assay revealed that four members of the nucleotide sugar transporter (NST) family can efficiently transport UDP-Ara f in vitro. Subsequent analysis of mutant lines affected in the function of these NSTs confirmed their role as UDP-Ara f transporters in vivo.},
doi = {10.1073/pnas.1701894114},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 16,
volume = 114,
place = {United States},
year = {2017},
month = {4}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1073/pnas.1701894114

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Cited by: 12 works
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