Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses
Abstract
Drought is the most important environmental stress limiting crop yields. The C4 cereal sorghum [Sorghum bicolor(L.) Moench] is a critical food, forage, and emerging bioenergy crop that is notably drought-tolerant. We conducted a large-scale field experiment, imposing preflowering and postflowering drought stress on 2 genotypes of sorghum across a tightly resolved time series, from plant emergence to postanthesis, resulting in a dataset of nearly 400 transcriptomes. We observed a fast and global transcriptomic response in leaf and root tissues with clear temporal patterns, including modulation of well-known drought pathways. We also identified genotypic differences in core photosynthesis and reactive oxygen species scavenging pathways, highlighting possible mechanisms of drought tolerance and of the delayed senescence, characteristic of the stay-green phenotype. Finally, we discovered a large-scale depletion in the expression of genes critical to arbuscular mycorrhizal (AM) symbiosis, with a corresponding drop in AM fungal mass in the plants’ roots.
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- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Univ. of California, Berkeley, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1577228
- Alternate Identifier(s):
- OSTI ID: 1582406; OSTI ID: 1591847; OSTI ID: 1660760
- Report Number(s):
- PNNL-SA-140530
Journal ID: ISSN 0027-8424
- Grant/Contract Number:
- SC0014081; SC0012460; AC02-05CH11231; AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; Drought; RNA-Seq; S. bicolorarbuscular mycorrhizal fungi
Citation Formats
Varoquaux, Nelle, Cole, Benjamin, Gao, Cheng, Pierroz, Grady, Baker, Christopher R., Patel, Dhruv, Madera, Mary, Jeffers, Tim, Hollingsworth, Joy, Sievert, Julie, Yoshinaga, Yuko, Owiti, Judith A., Singan, Vasanth R., DeGraaf, Stephanie, Xu, Ling, Blow, Matthew J., Harrison, Maria J., Visel, Axel, Jansson, Christer, Niyogi, Krishna K., Hutmacher, Robert, Coleman-Derr, Devin, O’Malley, Ronan C., Taylor, John W., Dahlberg, Jeffery, Vogel, John P., Lemaux, Peggy G., and Purdom, Elizabeth. Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses. United States: N. p., 2019.
Web. doi:10.1073/pnas.1907500116.
Varoquaux, Nelle, Cole, Benjamin, Gao, Cheng, Pierroz, Grady, Baker, Christopher R., Patel, Dhruv, Madera, Mary, Jeffers, Tim, Hollingsworth, Joy, Sievert, Julie, Yoshinaga, Yuko, Owiti, Judith A., Singan, Vasanth R., DeGraaf, Stephanie, Xu, Ling, Blow, Matthew J., Harrison, Maria J., Visel, Axel, Jansson, Christer, Niyogi, Krishna K., Hutmacher, Robert, Coleman-Derr, Devin, O’Malley, Ronan C., Taylor, John W., Dahlberg, Jeffery, Vogel, John P., Lemaux, Peggy G., & Purdom, Elizabeth. Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses. United States. https://doi.org/10.1073/pnas.1907500116
Varoquaux, Nelle, Cole, Benjamin, Gao, Cheng, Pierroz, Grady, Baker, Christopher R., Patel, Dhruv, Madera, Mary, Jeffers, Tim, Hollingsworth, Joy, Sievert, Julie, Yoshinaga, Yuko, Owiti, Judith A., Singan, Vasanth R., DeGraaf, Stephanie, Xu, Ling, Blow, Matthew J., Harrison, Maria J., Visel, Axel, Jansson, Christer, Niyogi, Krishna K., Hutmacher, Robert, Coleman-Derr, Devin, O’Malley, Ronan C., Taylor, John W., Dahlberg, Jeffery, Vogel, John P., Lemaux, Peggy G., and Purdom, Elizabeth. Thu .
"Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses". United States. https://doi.org/10.1073/pnas.1907500116.
@article{osti_1577228,
title = {Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses},
author = {Varoquaux, Nelle and Cole, Benjamin and Gao, Cheng and Pierroz, Grady and Baker, Christopher R. and Patel, Dhruv and Madera, Mary and Jeffers, Tim and Hollingsworth, Joy and Sievert, Julie and Yoshinaga, Yuko and Owiti, Judith A. and Singan, Vasanth R. and DeGraaf, Stephanie and Xu, Ling and Blow, Matthew J. and Harrison, Maria J. and Visel, Axel and Jansson, Christer and Niyogi, Krishna K. and Hutmacher, Robert and Coleman-Derr, Devin and O’Malley, Ronan C. and Taylor, John W. and Dahlberg, Jeffery and Vogel, John P. and Lemaux, Peggy G. and Purdom, Elizabeth},
abstractNote = {Drought is the most important environmental stress limiting crop yields. The C4 cereal sorghum [Sorghum bicolor(L.) Moench] is a critical food, forage, and emerging bioenergy crop that is notably drought-tolerant. We conducted a large-scale field experiment, imposing preflowering and postflowering drought stress on 2 genotypes of sorghum across a tightly resolved time series, from plant emergence to postanthesis, resulting in a dataset of nearly 400 transcriptomes. We observed a fast and global transcriptomic response in leaf and root tissues with clear temporal patterns, including modulation of well-known drought pathways. We also identified genotypic differences in core photosynthesis and reactive oxygen species scavenging pathways, highlighting possible mechanisms of drought tolerance and of the delayed senescence, characteristic of the stay-green phenotype. Finally, we discovered a large-scale depletion in the expression of genes critical to arbuscular mycorrhizal (AM) symbiosis, with a corresponding drop in AM fungal mass in the plants’ roots.},
doi = {10.1073/pnas.1907500116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {12}
}
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https://doi.org/10.1073/pnas.1907500116
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