Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses

Varoquaux, Nelle; Cole, Benjamin; Gao, Cheng; Pierroz, Grady; Baker, Christopher R.; Patel, Dhruv; Madera, Mary; Jeffers, Tim; Hollingsworth, Joy; Sievert, Julie; Yoshinaga, Yuko; Owiti, Judith A.; Singan, Vasanth R.; DeGraaf, Stephanie; Xu, Ling; Blow, Matthew J.; Harrison, Maria J.; Visel, Axel; Jansson, Christer; Niyogi, Krishna K.; Hutmacher, Robert; Coleman-Derr, Devin; O’Malley, Ronan C.; Taylor, John W.; Dahlberg, Jeffery; Vogel, John P.; Lemaux, Peggy G.; Purdom, Elizabeth

doi:10.1073/pnas.1907500116

Title: Transcriptomic analysis of field-droughted sorghum from seedling to maturity reveals biotic and metabolic responses

Journal Article · 04 December 2019 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1907500116 · OSTI ID:1577228

; Cole, Benjamin;

; Pierroz, Grady; Baker, Christopher R.;

; Madera, Mary; Jeffers, Tim; Hollingsworth, Joy; Sievert, Julie;

; Owiti, Judith A.; Singan, Vasanth R.; DeGraaf, Stephanie; Xu, Ling; Blow, Matthew J.; Harrison, Maria J.; Visel, Axel;

;

Drought is the most important environmental stress limiting crop yields. The C4 cereal sorghum [ Sorghum bicolor (L.) Moench] is a critical food, forage, and emerging bioenergy crop that is notably drought-tolerant. We conducted a large-scale field experiment, imposing preflowering and postflowering drought stress on 2 genotypes of sorghum across a tightly resolved time series, from plant emergence to postanthesis, resulting in a dataset of nearly 400 transcriptomes. We observed a fast and global transcriptomic response in leaf and root tissues with clear temporal patterns, including modulation of well-known drought pathways. We also identified genotypic differences in core photosynthesis and reactive oxygen species scavenging pathways, highlighting possible mechanisms of drought tolerance and of the delayed senescence, characteristic of the stay-green phenotype. Finally, we discovered a large-scale depletion in the expression of genes critical to arbuscular mycorrhizal (AM) symbiosis, with a corresponding drop in AM fungal mass in the plants’ roots.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0014081; SC0012460; AC02-05CH11231

OSTI ID:: 1577228

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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