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Title: A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells

Abstract

Fluorescent aptamer sensors have shown enormous potential for intracellular imaging of small molecule metabolites. Since metabolites distribute differently at different subcellular locations and their concentrations and locations fluctuate with time, methods are needed for spatiotemporally controlled monitoring of these metabolites. Built upon previous success in temporal control of aptamer-based sensors, we herein report an aptamer sensor containing a photocleavable linker and using DQAsomes to target mitochondria for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells. The photocleavable modification on the DNA ATP aptamer sensor can prevent sensor activation before reaching mitochondria and the sensor can then be activated upon light irradiation. The sensor has a detection limit of 3.7 μM and high selectivity against other nucleotides, allowing detection of ATP concentration fluctuations in mitochondria induced by Ca 2+ or oligomycin. This work represents the first successful delivery of a DNA aptamer sensor to mitochondria, providing a new platform for targeted delivery to subcellular organelles for monitoring energy producing processes, as well as mitochondrial dysfunction-related diseases in different cells.

Authors:
 [1];  [2];  [3]; ORCiD logo [3]; ORCiD logo [2]; ORCiD logo [4]; ORCiD logo [3]
  1. Chinese Academy of Sciences (CAS), Suzhou (China). Suzhou Inst. of Nano-Tech and Nano-Bionics, CAS Key Lab. of Nano-Bio Interface; Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry
  2. Nanjing Univ. (China). School of Chemistry and Chemical Engineering, State Key Lab. of Coordination Chemistry
  3. Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry, and DOE Center for Advanced Bioenergy and Biproducts Innovation
  4. Chinese Academy of Sciences (CAS), Suzhou (China). Suzhou Inst. of Nano-Tech and Nano-Bionics, CAS Key Lab. of Nano-Bio Interface
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1577186
Alternate Identifier(s):
OSTI ID: 1577854
Grant/Contract Number:  
SC0018420
Resource Type:
Published Article
Journal Name:
Chemical Science
Additional Journal Information:
Journal Name: Chemical Science; Journal ID: ISSN 2041-6520
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English

Citation Formats

Hong, Shanni, Zhang, Xiaoting, Lake, Ryan J., Pawel, Gregory T., Guo, Zijian, Pei, Renjun, and Lu, Yi. A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells. United States: N. p., 2019. Web. doi:10.1039/c9sc04773e.
Hong, Shanni, Zhang, Xiaoting, Lake, Ryan J., Pawel, Gregory T., Guo, Zijian, Pei, Renjun, & Lu, Yi. A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells. United States. doi:10.1039/c9sc04773e.
Hong, Shanni, Zhang, Xiaoting, Lake, Ryan J., Pawel, Gregory T., Guo, Zijian, Pei, Renjun, and Lu, Yi. Tue . "A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells". United States. doi:10.1039/c9sc04773e.
@article{osti_1577186,
title = {A photo-regulated aptamer sensor for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells},
author = {Hong, Shanni and Zhang, Xiaoting and Lake, Ryan J. and Pawel, Gregory T. and Guo, Zijian and Pei, Renjun and Lu, Yi},
abstractNote = {Fluorescent aptamer sensors have shown enormous potential for intracellular imaging of small molecule metabolites. Since metabolites distribute differently at different subcellular locations and their concentrations and locations fluctuate with time, methods are needed for spatiotemporally controlled monitoring of these metabolites. Built upon previous success in temporal control of aptamer-based sensors, we herein report an aptamer sensor containing a photocleavable linker and using DQAsomes to target mitochondria for spatiotemporally controlled monitoring of ATP in the mitochondria of living cells. The photocleavable modification on the DNA ATP aptamer sensor can prevent sensor activation before reaching mitochondria and the sensor can then be activated upon light irradiation. The sensor has a detection limit of 3.7 μM and high selectivity against other nucleotides, allowing detection of ATP concentration fluctuations in mitochondria induced by Ca2+ or oligomycin. This work represents the first successful delivery of a DNA aptamer sensor to mitochondria, providing a new platform for targeted delivery to subcellular organelles for monitoring energy producing processes, as well as mitochondrial dysfunction-related diseases in different cells.},
doi = {10.1039/c9sc04773e},
journal = {Chemical Science},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
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DOI: 10.1039/c9sc04773e

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