Title: On Modeling Ensemble Transport of Metal Reducing Motile Bacteria

Abstract

Accurate numerical models of bioremediation are needed to support design and evaluation of field implementations. While several models of bacterial processes do not consider their ensemble motion, some models treat motility using an advection diffusion equation (ADE). In this study, Geobacter and Pelosinus, two metal reducing species, are used in micromodel experiments for study of their motility characteristics, as a prelude to development and testing of new models of bacterial transport during bioremediation. A sequence of experiments was conducted to quantify the two-dimensional movement patterns of individual cells in micro-models in the absence of flow. Trajectories of individual cells on the order of several seconds to few minutes in duration are analyzed to provide information on (1) the length of runs, and (2) time needed to complete a run (waiting or residence time). A Continuous Time Random Walk (CTRW) model to predict ensemble breakthrough plots is developed based on the motility statistics. The results of the CTRW model and an ADE model are compared with the real breakthrough plots obtained directly from the trajectories. The ADE model is shown to be insufficient, whereas a coupled CTRW model is found to be good at predicting breakthroughs at short distances and atmore » early times, but not at late time and long distances. The inadequacies of the simple CTRW model can possibly be improved by accounting for correlation in run length and waiting time.« less

Authors:

Yang, Xueke; Parashar, Rishi; Sund, Nicole L.; Plymale, Andrew E.; Scheibe, Timothy D. ;

• Search DOE PAGES for author "Scheibe, Timothy D."
• Search DOE PAGES for ORCID "0000-0002-8864-5772"

Hu, Dehong ;

• Search DOE PAGES for author "Hu, Dehong"
• Search DOE PAGES for ORCID "0000-0002-3974-2963"

Kelly, Ryan T.

Publication Date:

Thu Oct 10 00:00:00 EDT 2019

Research Org.:

Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Nevada System of Higher Education (NSHE) - Desert Research Institute, Reno, NV (United States)

Sponsoring Org.:

USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Org.:

Environmental Molecular Sciences Laboratory (Pacific Northwest National Lab)

OSTI Identifier:

1619610

Alternate Identifier(s):

OSTI ID: 1577077; OSTI ID: 1659517

Report Number(s):

PNNL-SA-134947
Journal ID: ISSN 2045-2322; 14638; PII: 51271

Grant/Contract Number:  

SC0019437; AC05-76RL01830

Resource Type:

Published Article

Journal Name:

Scientific Reports

Additional Journal Information:

Journal Name: Scientific Reports Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 2045-2322

Publisher:

Nature Publishing Group

Country of Publication:

United Kingdom

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; 60 APPLIED LIFE SCIENCES; Microbial Motility; Bioremediation; Transport Models