Structure and activity of PPX/GppA homologs from Escherichia coli and Helicobacter pylori

Song, He; Dharmasena, Madushini N.; Wang, Chao; Shaw, Gary X.; Cherry, Scott; Tropea, Joseph E.; Jin, Ding J.; Ji, Xinhua

doi:10.1111/febs.15120

Title: Structure and activity of PPX/GppA homologs from Escherichia coli and Helicobacter pylori

Journal Article · 23 November 2019 · Federation of European Biochemical Societies (FEBS) Journal

DOI: https://doi.org/10.1111/febs.15120 · OSTI ID:1575312

Song, He ^[1]; Dharmasena, Madushini N. ^[2]; Wang, Chao ^[1]; Shaw, Gary X. ^[1]; Cherry, Scott ^[1]; Tropea, Joseph E. ^[1]; Jin, Ding J. ^[2];

^[1]

Macromolecular Crystallography Laboratory National Cancer Institute Frederick MD USA
RNA Biology Laboratory National Cancer Institute Frederick MD USA

Rapid adaptation to environmental changes is crucial for bacterial survival. Almost all bacteria possess a conserved stringent response system to prompt transcriptional and metabolic responses toward stress. The adaptive process relies on alarmones, guanosine pentaphosphate (pppGpp), and tetraphosphate (ppGpp), to regulate global gene expression. The ppGpp is more potent than pppGpp in the regulatory activity, and pppGpp phosphohydrolase (GppA) plays a key role in (p)ppGpp homeostasis. Sharing a similar domain structure, GppA is indistinguishable from exopolyphosphatase (PPX), which mediates the metabolism of cellular inorganic polyphosphate. Here, our phylogenetic analysis of PPX/GppA homologs in bacteria shows a wide distribution with several distinct subfamilies, and our structural and functional analysis of Escherichia coli GppA and Helicobacter pylori PPX/GppA reveals unique properties of each homolog. These results explain how each homolog possesses its distinct functionality.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1575312

Journal Information:: Federation of European Biochemical Societies (FEBS) Journal, Journal Name: Federation of European Biochemical Societies (FEBS) Journal Journal Issue: 9 Vol. 287; ISSN 1742-464X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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