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Title: Light-Stress Influences the Composition of the Murine Gut Microbiome, Memory Function, and Plasma Metabolome

Abstract

The gut microbiome plays an important role in the mammalian host and when in proper balance helps protect health and prevent disease. Host environmental stress and its influence on the gut microbiome, health and disease is an emerging area of research. Exposures to unnatural light cycles are becoming increasingly common due to travel and shift work. However, much remains unknown about how these changes influence the microbiome and host health. This information is needed to understand and predict the relationship between the microbiome and host response to altered sleep cycles. In the present study, we exposed three cohorts of mice to different light cycle regimens for 12 consecutive weeks; including continuous light, continuous dark and a standard light dark regimen consisting of 12 hours light followed by 12 hours of dark. After exposure, motor and memory behavior, and the composition of the fecal microbiome and plasma metabolome were measured. Memory potential was significantly reduced in mice exposed to continuous light, whereas rotarod performance was minimally affected. The overall structure of the microbiome was relatively constant over time. However, Bacteroidales Rikenellaceae was relatively more abundant in mice exposed to continuous dark, while Bacteroidales S24-7 was relatively more abundant in mice exposedmore » to continuous light. Several plasma metabolites, including glucose, tryptophan, pyruvate and several unidentified metabolites, were correlated to continuous dark and light exposure conditions. Specific metabolites were correlated to the abundances of specific microorganisms based on light exposure. This study provides the foundation for future studies into the mechanisms underlying the role of the gut microbiome on the murine host during light-dark stress.« less

Authors:
ORCiD logo [1];  [2]; ORCiD logo [1]; ORCiD logo [3]; ORCiD logo [1];  [1]; ORCiD logo [1];  [2];  [1]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Biological Sciences Div.
  2. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Div.
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Computing and Analytics Div.
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1574891
Report Number(s):
PNNL-SA-144001
Journal ID: ISSN 2296-889X
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Molecular Biosciences
Additional Journal Information:
Journal Volume: 6; Journal ID: ISSN 2296-889X
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; light stress; sleep cycle; gut microbiome; plasma metabolome; behavior change; memory function

Citation Formats

Kim, Young -Mo, Snijders, Antoine M., Brislawn, Colin J., Stratton, Kelly G., Zink, Erika M., Fansler, Sarah J., Metz, Thomas O., Mao, Jian -Hua, and Jansson, Janet K. Light-Stress Influences the Composition of the Murine Gut Microbiome, Memory Function, and Plasma Metabolome. United States: N. p., 2019. Web. doi:10.3389/fmolb.2019.00108.
Kim, Young -Mo, Snijders, Antoine M., Brislawn, Colin J., Stratton, Kelly G., Zink, Erika M., Fansler, Sarah J., Metz, Thomas O., Mao, Jian -Hua, & Jansson, Janet K. Light-Stress Influences the Composition of the Murine Gut Microbiome, Memory Function, and Plasma Metabolome. United States. doi:10.3389/fmolb.2019.00108.
Kim, Young -Mo, Snijders, Antoine M., Brislawn, Colin J., Stratton, Kelly G., Zink, Erika M., Fansler, Sarah J., Metz, Thomas O., Mao, Jian -Hua, and Jansson, Janet K. Fri . "Light-Stress Influences the Composition of the Murine Gut Microbiome, Memory Function, and Plasma Metabolome". United States. doi:10.3389/fmolb.2019.00108. https://www.osti.gov/servlets/purl/1574891.
@article{osti_1574891,
title = {Light-Stress Influences the Composition of the Murine Gut Microbiome, Memory Function, and Plasma Metabolome},
author = {Kim, Young -Mo and Snijders, Antoine M. and Brislawn, Colin J. and Stratton, Kelly G. and Zink, Erika M. and Fansler, Sarah J. and Metz, Thomas O. and Mao, Jian -Hua and Jansson, Janet K.},
abstractNote = {The gut microbiome plays an important role in the mammalian host and when in proper balance helps protect health and prevent disease. Host environmental stress and its influence on the gut microbiome, health and disease is an emerging area of research. Exposures to unnatural light cycles are becoming increasingly common due to travel and shift work. However, much remains unknown about how these changes influence the microbiome and host health. This information is needed to understand and predict the relationship between the microbiome and host response to altered sleep cycles. In the present study, we exposed three cohorts of mice to different light cycle regimens for 12 consecutive weeks; including continuous light, continuous dark and a standard light dark regimen consisting of 12 hours light followed by 12 hours of dark. After exposure, motor and memory behavior, and the composition of the fecal microbiome and plasma metabolome were measured. Memory potential was significantly reduced in mice exposed to continuous light, whereas rotarod performance was minimally affected. The overall structure of the microbiome was relatively constant over time. However, Bacteroidales Rikenellaceae was relatively more abundant in mice exposed to continuous dark, while Bacteroidales S24-7 was relatively more abundant in mice exposed to continuous light. Several plasma metabolites, including glucose, tryptophan, pyruvate and several unidentified metabolites, were correlated to continuous dark and light exposure conditions. Specific metabolites were correlated to the abundances of specific microorganisms based on light exposure. This study provides the foundation for future studies into the mechanisms underlying the role of the gut microbiome on the murine host during light-dark stress.},
doi = {10.3389/fmolb.2019.00108},
journal = {Frontiers in Molecular Biosciences},
number = ,
volume = 6,
place = {United States},
year = {2019},
month = {10}
}

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