Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic

Rusley, Calvin; Onstott, Tullis C.; Vishnivetskaya, Tatiana A.; Layton, Alice; Chauhan, Archana; Pfiffner, Susan M.; Whyte, Lyle G.; Lau, Maggie C. Y.; Cameron Thrash, ed., J.

doi:10.1128/MRA.01178-19

Title: Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic

Abstract

Metagenomic sequencing of active-layer cryosols from the Canadian High Arctic has yielded a nearly complete genome for an atmospheric CH ₄ -oxidizing bacterium belonging to upland soil cluster α (USCα). This genome contains genes involved in CH ₄ metabolism, H ₂ metabolism, and multiple carbon assimilation pathways.

Authors:

Rusley, Calvin ^[1];

^[1];

^[2]; Layton, Alice ^[2];

^[2]; Pfiffner, Susan M. ^[2]; Whyte, Lyle G. ^[3];

^[1]; Cameron Thrash, ed., J.

Department of Geosciences, Princeton University, Princeton, New Jersey, USA
Center for Environmental Biotechnology, University of Tennessee, Knoxville, Tennessee, USA
Department of Natural Resource Sciences, McGill University, Montreal, Québec, Canada

Publication Date:: 2019-11-14

Sponsoring Org.:: USDOE

OSTI Identifier:: 1574060

Grant/Contract Number:: SC0004902

Resource Type:: Published Article

Journal Name:: Microbiology Resource Announcements

Additional Journal Information:: Journal Name: Microbiology Resource Announcements Journal Volume: 8 Journal Issue: 46; Journal ID: ISSN 2576-098X

Publisher:: American Society for Microbiology

Country of Publication:: United States

Language:: English

Citation Formats


                    Rusley, Calvin, Onstott, Tullis C., Vishnivetskaya, Tatiana A., Layton, Alice, Chauhan, Archana, Pfiffner, Susan M., Whyte, Lyle G., Lau, Maggie C. Y., and Cameron Thrash, ed., J. Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic.  United States: N. p., 2019. 
Web.  doi:10.1128/MRA.01178-19.

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                    Rusley, Calvin, Onstott, Tullis C., Vishnivetskaya, Tatiana A., Layton, Alice, Chauhan, Archana, Pfiffner, Susan M., Whyte, Lyle G., Lau, Maggie C. Y., & Cameron Thrash, ed., J. Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic.  United States.  https://doi.org/10.1128/MRA.01178-19

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                    Rusley, Calvin, Onstott, Tullis C., Vishnivetskaya, Tatiana A., Layton, Alice, Chauhan, Archana, Pfiffner, Susan M., Whyte, Lyle G., Lau, Maggie C. Y., and Cameron Thrash, ed., J. Thu .  
"Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic".  United States.  https://doi.org/10.1128/MRA.01178-19.

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@article{osti_1574060,

  title        = {Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic},

  author       = {Rusley, Calvin and Onstott, Tullis C. and Vishnivetskaya, Tatiana A. and Layton, Alice and Chauhan, Archana and Pfiffner, Susan M. and Whyte, Lyle G. and Lau, Maggie C. Y. and Cameron Thrash, ed., J.},

  abstractNote = {Metagenomic sequencing of active-layer cryosols from the Canadian High Arctic has yielded a nearly complete genome for an atmospheric CH 4 -oxidizing bacterium belonging to upland soil cluster α (USCα). This genome contains genes involved in CH 4 metabolism, H 2 metabolism, and multiple carbon assimilation pathways.},

  doi          = {10.1128/MRA.01178-19},

  journal      = {Microbiology Resource Announcements},

  number       = 46,

  volume       = 8,

  place        = {United States},

  year         = {2019},

  month        = {11}

}

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Title: Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic

Abstract

Citation Formats

Widespread soil bacterium that oxidizes atmospheric methane journal, April 2019

IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth journal, April 2012

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Anvi’o: an advanced analysis and visualization platform for ‘omics data journal, January 2015

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Effects of simulated spring thaw of permafrost from mineral cryosol on CO 2 emissions and atmospheric CH 4 uptake journal, September 2015

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Fast gapped-read alignment with Bowtie 2 journal, March 2012

BLAST+: architecture and applications journal, January 2009

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An active atmospheric methane sink in high Arctic mineral cryosols journal, April 2015

Metagenomes from Thawing Low-Soil-Organic-Carbon Mineral Cryosols and Permafrost of the Canadian High Arctic journal, November 2014

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journal, April 2019

IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth
journal, April 2012

Prokka: rapid prokaryotic genome annotation
journal, March 2014

Anvi’o: an advanced analysis and visualization platform for ‘omics data
journal, January 2015

SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
journal, May 2012

MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities
journal, January 2015

KAAS: an automatic genome annotation and pathway reconstruction server
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journal, March 2012

BLAST+: architecture and applications
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