Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic

doi:10.1128/MRA.01178-19

Title: Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic

Abstract

ABSTRACT Metagenomic sequencing of active-layer cryosols from the Canadian High Arctic has yielded a nearly complete genome for an atmospheric CH ₄ -oxidizing bacterium belonging to upland soil cluster α (USCα). This genome contains genes involved in CH ₄ metabolism, H ₂ metabolism, and multiple carbon assimilation pathways.

Authors:

Rusley, Calvin;

;

; Layton, Alice;

; Pfiffner, Susan M.; Whyte, Lyle G.;

; Cameron Thrash, ed., J.

Publication Date:: 2019-11-14

Sponsoring Org.:: USDOE

OSTI Identifier:: 1574060

Grant/Contract Number:: SC0004902

Resource Type:: Published Article

Journal Name:: Microbiology Resource Announcements

Additional Journal Information:: Journal Name: Microbiology Resource Announcements Journal Volume: 8 Journal Issue: 46; Journal ID: ISSN 2576-098X

Publisher:: American Society for Microbiology

Country of Publication:: United States

Language:: English

Citation Formats


                    Rusley, Calvin, Onstott, Tullis C., Vishnivetskaya, Tatiana A., Layton, Alice, Chauhan, Archana, Pfiffner, Susan M., Whyte, Lyle G., Lau, Maggie C. Y., and Cameron Thrash, ed., J. Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic.  United States: N. p., 2019. 
        Web.  doi:10.1128/MRA.01178-19.

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                    Rusley, Calvin, Onstott, Tullis C., Vishnivetskaya, Tatiana A., Layton, Alice, Chauhan, Archana, Pfiffner, Susan M., Whyte, Lyle G., Lau, Maggie C. Y., & Cameron Thrash, ed., J. Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic.  United States.  doi:10.1128/MRA.01178-19.

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                    Rusley, Calvin, Onstott, Tullis C., Vishnivetskaya, Tatiana A., Layton, Alice, Chauhan, Archana, Pfiffner, Susan M., Whyte, Lyle G., Lau, Maggie C. Y., and Cameron Thrash, ed., J. Thu .  
        "Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic".  United States.  doi:10.1128/MRA.01178-19.

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@article{osti_1574060,

  title        = {Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic},

  author       = {Rusley, Calvin and Onstott, Tullis C. and Vishnivetskaya, Tatiana A. and Layton, Alice and Chauhan, Archana and Pfiffner, Susan M. and Whyte, Lyle G. and Lau, Maggie C. Y. and Cameron Thrash, ed., J.},

  abstractNote = {ABSTRACT Metagenomic sequencing of active-layer cryosols from the Canadian High Arctic has yielded a nearly complete genome for an atmospheric CH 4 -oxidizing bacterium belonging to upland soil cluster α (USCα). This genome contains genes involved in CH 4 metabolism, H 2 metabolism, and multiple carbon assimilation pathways.},

  doi          = {10.1128/MRA.01178-19},

  journal      = {Microbiology Resource Announcements},

  number       = 46,

  volume       = 8,

  place        = {United States},

  year         = {2019},

  month        = {11}

}

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DOI: 10.1128/MRA.01178-19

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Works referenced in this record:

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Title: Metagenome-Assembled Genome of USCα AHI, a Potential High-Affinity Methanotroph from Axel Heiberg Island, Canadian High Arctic

Abstract

Citation Formats

Widespread soil bacterium that oxidizes atmospheric methane journal, April 2019

IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth journal, April 2012

Prokka: rapid prokaryotic genome annotation journal, March 2014

Anvi’o: an advanced analysis and visualization platform for ‘omics data journal, January 2015

SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing journal, May 2012

MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities journal, January 2015

KAAS: an automatic genome annotation and pathway reconstruction server journal, May 2007

CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes journal, May 2015

Insights into the phylogeny and coding potential of microbial dark matter journal, July 2013

Effects of simulated spring thaw of permafrost from mineral cryosol on CO 2 emissions and atmospheric CH 4 uptake journal, September 2015

Atmospheric CH 4 oxidation by Arctic permafrost and mineral cryosols as a function of water saturation and temperature journal, July 2016

Fast gapped-read alignment with Bowtie 2 journal, March 2012

BLAST+: architecture and applications journal, January 2009

Unravelling the Identity, Metabolic Potential and Global Biogeography of the Atmospheric Methane-Oxidizing Upland Soil Cluster α: Illuminating the USCα methanotrophs from soil journal, January 2018

An active atmospheric methane sink in high Arctic mineral cryosols journal, April 2015

Metagenomes from Thawing Low-Soil-Organic-Carbon Mineral Cryosols and Permafrost of the Canadian High Arctic journal, November 2014

Widespread soil bacterium that oxidizes atmospheric methane
journal, April 2019

IDBA-UD: a de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth
journal, April 2012

Prokka: rapid prokaryotic genome annotation
journal, March 2014

Anvi’o: an advanced analysis and visualization platform for ‘omics data
journal, January 2015

SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing
journal, May 2012

MetaBAT, an efficient tool for accurately reconstructing single genomes from complex microbial communities
journal, January 2015

KAAS: an automatic genome annotation and pathway reconstruction server
journal, May 2007

CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes
journal, May 2015

Insights into the phylogeny and coding potential of microbial dark matter
journal, July 2013

Effects of simulated spring thaw of permafrost from mineral cryosol on CO ₂ emissions and atmospheric CH ₄ uptake
journal, September 2015

Atmospheric CH ₄ oxidation by Arctic permafrost and mineral cryosols as a function of water saturation and temperature
journal, July 2016

Fast gapped-read alignment with Bowtie 2
journal, March 2012

BLAST+: architecture and applications
journal, January 2009

Unravelling the Identity, Metabolic Potential and Global Biogeography of the Atmospheric Methane-Oxidizing Upland Soil Cluster α: Illuminating the USCα methanotrophs from soil
journal, January 2018

An active atmospheric methane sink in high Arctic mineral cryosols
journal, April 2015

Metagenomes from Thawing Low-Soil-Organic-Carbon Mineral Cryosols and Permafrost of the Canadian High Arctic
journal, November 2014