Chemical and structural analysis of a photoactive vertebrate cryptochrome from pigeon

Zoltowski, Brian D.; Chelliah, Yogarany; Wickramaratne, Anushka; Jarocha, Lauren; Karki, Nischal; Xu, Wei; Mouritsen, Henrik; Hore, Peter J.; Hibbs, Ryan E.; Green, Carla B.; Takahashi, Joseph S.

doi:10.1073/pnas.1907875116

Title: Chemical and structural analysis of a photoactive vertebrate cryptochrome from pigeon

Journal Article · 03 September 2019 · Proceedings of the National Academy of Sciences of the United States of America

DOI: https://doi.org/10.1073/pnas.1907875116 · OSTI ID:1573376

Zoltowski, Brian D. ^[1]; Chelliah, Yogarany ^[2]; Wickramaratne, Anushka ^[2]; Jarocha, Lauren ^[3]; Karki, Nischal ^[4]; Xu, Wei ^[2]; Mouritsen, Henrik ^[5]; Hore, Peter J. ^[3]; Hibbs, Ryan E. ^[2];

^[2];

^[2]

Southern Methodist Univ., Dallas, TX (United States); DOE/OSTI
Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
Univ. of Oxford (United Kingdom)
Southern Methodist Univ., Dallas, TX (United States)
Carl von Ossietzky Univ., Oldenburg (Germany)

Computational and biochemical studies implicate the blue-light sensor cryptochrome (CRY) as an endogenous light-dependent magnetosensor enabling migratory birds to navigate using the Earth’s magnetic field. Validation of such a mechanism has been hampered by the absence of structures of vertebrate CRYs that have functional photochemistry. Here we present crystal structures of Columba livia (pigeon) CRY4 that reveal evolutionarily conserved modifications to a sequence of Trp residues (Trp-triad) required for CRY photoreduction. In ClCRY4, the Trp-triad chain is extended to include a fourth Trp (W369) and a Tyr (Y319) residue at the protein surface that imparts an unusually high quantum yield of photoreduction. These results are consistent with observations of night migratory behavior in animals at low light levels and could have implications for photochemical pathways allowing magnetosensing.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC02-06CH11357; AC02-05CH11231

OSTI ID:: 1573376

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Issue: 39 Vol. 116; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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