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Title: Reduction-dependent siderophore assimilation in a model pennate diatom

Abstract

Iron uptake by diatoms is a biochemical process with global biogeochemical implications. In large regions of the surface ocean diatoms are both responsible for the majority of primary production and frequently experiencing iron limitation of growth. The strategies used by these phytoplankton to extract iron from seawater constrain carbon flux into higher trophic levels and sequestration into sediments. In this study we use reverse genetic techniques to target putative iron-acquisition genes in the model pennate diatom Phaeodactylum tricornutum . We describe components of a reduction-dependent siderophore acquisition pathway that relies on a bacterial-derived receptor protein and provides a viable alternative to inorganic iron uptake under certain conditions. This form of iron uptake entails a close association between diatoms and siderophore-producing organisms during low-iron conditions. Homologs of these proteins are found distributed across diatom lineages, suggesting the significance of siderophore utilization by diatoms in the marine environment. Evaluation of specific proteins enables us to confirm independent iron-acquisition pathways in diatoms and characterize their preferred substrates. These findings refine our mechanistic understanding of the multiple iron-uptake systems used by diatoms and help us better predict the influence of iron speciation on taxa-specific iron bioavailability.

Authors:
ORCiD logo; ; ; ; ; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1573107
Grant/Contract Number:  
SC0018344
Resource Type:
Published Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 116 Journal Issue: 47; Journal ID: ISSN 0027-8424
Publisher:
Proceedings of the National Academy of Sciences
Country of Publication:
United States
Language:
English

Citation Formats

Coale, Tyler H., Moosburner, Mark, Horák, Aleš, Oborník, Miroslav, Barbeau, Katherine A., and Allen, Andrew E. Reduction-dependent siderophore assimilation in a model pennate diatom. United States: N. p., 2019. Web. doi:10.1073/pnas.1907234116.
Coale, Tyler H., Moosburner, Mark, Horák, Aleš, Oborník, Miroslav, Barbeau, Katherine A., & Allen, Andrew E. Reduction-dependent siderophore assimilation in a model pennate diatom. United States. doi:10.1073/pnas.1907234116.
Coale, Tyler H., Moosburner, Mark, Horák, Aleš, Oborník, Miroslav, Barbeau, Katherine A., and Allen, Andrew E. Mon . "Reduction-dependent siderophore assimilation in a model pennate diatom". United States. doi:10.1073/pnas.1907234116.
@article{osti_1573107,
title = {Reduction-dependent siderophore assimilation in a model pennate diatom},
author = {Coale, Tyler H. and Moosburner, Mark and Horák, Aleš and Oborník, Miroslav and Barbeau, Katherine A. and Allen, Andrew E.},
abstractNote = {Iron uptake by diatoms is a biochemical process with global biogeochemical implications. In large regions of the surface ocean diatoms are both responsible for the majority of primary production and frequently experiencing iron limitation of growth. The strategies used by these phytoplankton to extract iron from seawater constrain carbon flux into higher trophic levels and sequestration into sediments. In this study we use reverse genetic techniques to target putative iron-acquisition genes in the model pennate diatom Phaeodactylum tricornutum . We describe components of a reduction-dependent siderophore acquisition pathway that relies on a bacterial-derived receptor protein and provides a viable alternative to inorganic iron uptake under certain conditions. This form of iron uptake entails a close association between diatoms and siderophore-producing organisms during low-iron conditions. Homologs of these proteins are found distributed across diatom lineages, suggesting the significance of siderophore utilization by diatoms in the marine environment. Evaluation of specific proteins enables us to confirm independent iron-acquisition pathways in diatoms and characterize their preferred substrates. These findings refine our mechanistic understanding of the multiple iron-uptake systems used by diatoms and help us better predict the influence of iron speciation on taxa-specific iron bioavailability.},
doi = {10.1073/pnas.1907234116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 47,
volume = 116,
place = {United States},
year = {2019},
month = {11}
}

Journal Article:
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DOI: 10.1073/pnas.1907234116

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