Starch Deficiency Enhances Lipid Biosynthesis and Turnover in Leaves
Abstract
Starch and lipids represent two major forms of carbon and energy storage in plants, and play central roles in diverse cellular processes. However, whether and how starch and lipid metabolic pathways interact to regulate metabolism and growth is poorly understood. In this paper, we show that lipids can partially compensate for the lack of function of transient starch during normal growth and development in Arabidopsis (Arabidopsis thaliana). Disruption of starch synthesis resulted in a significant increase in fatty acid synthesis via posttranslational regulation of the plastidic acetyl-CoA carboxylase and a concurrent increase in the synthesis and turnover of membrane lipids and triacylglycerol. Genetic analysis showed that blocking fatty acid peroxisomal β-oxidation, the sole pathway for metabolic breakdown of fatty acids in plants, significantly compromised or stunted the growth and development of mutants defective in starch synthesis under long days or short days, respectively. We also found that the combined disruption of starch synthesis and fatty acid turnover resulted in increased accumulation of membrane lipids, triacylglycerol and soluble sugars and altered fatty acid flux between the two lipid biosynthetic pathways compartmentalized in either the chloroplast or the endoplasmic reticulum. Finally and collectively, our findings provide insight into the role of fattymore »
- Authors:
-
[1];
[1];
[1];
[1]
- Biology Department, Brookhaven National Laboratory, Upton, New York 11973
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1573094
- Alternate Identifier(s):
- OSTI ID: 1464113
- Report Number(s):
- BNL-207940-2018-JAAM
Journal ID: ISSN 0032-0889; /plantphysiol/178/1/118.atom
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Published Article
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda) Journal Volume: 178 Journal Issue: 1; Journal ID: ISSN 0032-0889
- Publisher:
- American Society of Plant Biologists (ASPB)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Yu, Linhui, Fan, Jilian, Yan, Chengshi, and Xu, Changcheng. Starch Deficiency Enhances Lipid Biosynthesis and Turnover in Leaves. United States: N. p., 2018.
Web. doi:10.1104/pp.18.00539.
Yu, Linhui, Fan, Jilian, Yan, Chengshi, & Xu, Changcheng. Starch Deficiency Enhances Lipid Biosynthesis and Turnover in Leaves. United States. https://doi.org/10.1104/pp.18.00539
Yu, Linhui, Fan, Jilian, Yan, Chengshi, and Xu, Changcheng. Fri .
"Starch Deficiency Enhances Lipid Biosynthesis and Turnover in Leaves". United States. https://doi.org/10.1104/pp.18.00539.
@article{osti_1573094,
title = {Starch Deficiency Enhances Lipid Biosynthesis and Turnover in Leaves},
author = {Yu, Linhui and Fan, Jilian and Yan, Chengshi and Xu, Changcheng},
abstractNote = {Starch and lipids represent two major forms of carbon and energy storage in plants, and play central roles in diverse cellular processes. However, whether and how starch and lipid metabolic pathways interact to regulate metabolism and growth is poorly understood. In this paper, we show that lipids can partially compensate for the lack of function of transient starch during normal growth and development in Arabidopsis (Arabidopsis thaliana). Disruption of starch synthesis resulted in a significant increase in fatty acid synthesis via posttranslational regulation of the plastidic acetyl-CoA carboxylase and a concurrent increase in the synthesis and turnover of membrane lipids and triacylglycerol. Genetic analysis showed that blocking fatty acid peroxisomal β-oxidation, the sole pathway for metabolic breakdown of fatty acids in plants, significantly compromised or stunted the growth and development of mutants defective in starch synthesis under long days or short days, respectively. We also found that the combined disruption of starch synthesis and fatty acid turnover resulted in increased accumulation of membrane lipids, triacylglycerol and soluble sugars and altered fatty acid flux between the two lipid biosynthetic pathways compartmentalized in either the chloroplast or the endoplasmic reticulum. Finally and collectively, our findings provide insight into the role of fatty acid β-oxidation and the regulatory network controlling fatty acid synthesis, and reveal the mechanistic basis by which starch and lipid metabolic pathways interact and undergo crosstalk to modulate carbon allocation, energy homeostasis and plant growth.},
doi = {10.1104/pp.18.00539},
journal = {Plant Physiology (Bethesda)},
number = 1,
volume = 178,
place = {United States},
year = {Fri Aug 03 00:00:00 EDT 2018},
month = {Fri Aug 03 00:00:00 EDT 2018}
}
https://doi.org/10.1104/pp.18.00539
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