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Title: Engineering Clostridium for improved solvent production: recent progress and perspective

Abstract

Clostridia are Gram-positive, spore-forming, obligate anaerobic bacteria that can produce solvents such as acetone, ethanol, and butanol, which can be used as biofuels or building-block chemicals. Many successful attempts have been made to improve solvent yield and titer from sugars through metabolic engineering of solventogenic and acidogenic clostridia. More recently, cellulolytic and acetogenic clostridia have also attracted high interests for their ability to utilize low-cost renewable substrates such as cellulose and syngas. Process engineering such as in situ butanol recovery and consolidated bioprocessing (CBP) has been developed for improved solvent titer and productivity. This review focuses on metabolic and process engineering strategies for solvent production from sugars, lignocellulosic biomass, and syngas by various clostridia, including conventional solventogenic Clostridium acetobutylicum, engineered acidogens such as C. tyrobutyricum and C. cellulovorans, and carboxydotrophic acetogens such as C. carboxidivorans and C. ljungdahlii.

Authors:
 [1];  [2]; ORCiD logo [2]
  1. Dalian Univ. of Technology, Dalian (China); The Ohio State Univ., Columbus, OH (United States)
  2. The Ohio State Univ., Columbus, OH (United States)
Publication Date:
Research Org.:
The Ohio State Univ., Columbus, OH (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1572190
Grant/Contract Number:  
EE0007005
Resource Type:
Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 103; Journal Issue: 14; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Biofuels; butanol; solvent; clostridia; fermentation; metabolic engineering

Citation Formats

Cheng, Chi, Bao, Teng, and Yang, Shang-Tian. Engineering Clostridium for improved solvent production: recent progress and perspective. United States: N. p., 2019. Web. doi:10.1007/s00253-019-09916-7.
Cheng, Chi, Bao, Teng, & Yang, Shang-Tian. Engineering Clostridium for improved solvent production: recent progress and perspective. United States. doi:10.1007/s00253-019-09916-7.
Cheng, Chi, Bao, Teng, and Yang, Shang-Tian. Wed . "Engineering Clostridium for improved solvent production: recent progress and perspective". United States. doi:10.1007/s00253-019-09916-7. https://www.osti.gov/servlets/purl/1572190.
@article{osti_1572190,
title = {Engineering Clostridium for improved solvent production: recent progress and perspective},
author = {Cheng, Chi and Bao, Teng and Yang, Shang-Tian},
abstractNote = {Clostridia are Gram-positive, spore-forming, obligate anaerobic bacteria that can produce solvents such as acetone, ethanol, and butanol, which can be used as biofuels or building-block chemicals. Many successful attempts have been made to improve solvent yield and titer from sugars through metabolic engineering of solventogenic and acidogenic clostridia. More recently, cellulolytic and acetogenic clostridia have also attracted high interests for their ability to utilize low-cost renewable substrates such as cellulose and syngas. Process engineering such as in situ butanol recovery and consolidated bioprocessing (CBP) has been developed for improved solvent titer and productivity. This review focuses on metabolic and process engineering strategies for solvent production from sugars, lignocellulosic biomass, and syngas by various clostridia, including conventional solventogenic Clostridium acetobutylicum, engineered acidogens such as C. tyrobutyricum and C. cellulovorans, and carboxydotrophic acetogens such as C. carboxidivorans and C. ljungdahlii.},
doi = {10.1007/s00253-019-09916-7},
journal = {Applied Microbiology and Biotechnology},
number = 14,
volume = 103,
place = {United States},
year = {2019},
month = {5}
}

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Cited by: 11 works
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    • Applied Microbiology and Biotechnology, Vol. 101, Issue 10
    • DOI: 10.1007/s00253-017-8200-1

    Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans
    journal, November 2017


    Two-stage in situ gas stripping for enhanced butanol fermentation and energy-saving product recovery
    journal, May 2013


    The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications
    journal, August 2014


    Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum
    journal, June 2016


    Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
    journal, October 2016

    • Nagaraju, Shilpa; Davies, Naomi Kathleen; Walker, David Jeffrey Fraser
    • Biotechnology for Biofuels, Vol. 9, Issue 1
    • DOI: 10.1186/s13068-016-0638-3

    Restriction modification system analysis and development of in vivo methylation for the transformation of Clostridium cellulovorans
    journal, November 2015

    • Yang, Xiaorui; Xu, Mengmeng; Yang, Shang-Tian
    • Applied Microbiology and Biotechnology, Vol. 100, Issue 5
    • DOI: 10.1007/s00253-015-7141-9

    Recent progress in biobutanol tolerance in microbial systems with an emphasis on Clostridium
    journal, January 2016

    • Peabody, George L.; Kao, Katy C.
    • FEMS Microbiology Letters, Vol. 363, Issue 5
    • DOI: 10.1093/femsle/fnw017

    Effect of trace metals on ethanol production from synthesis gas by the ethanologenic acetogen, Clostridium ragsdalei
    journal, August 2010

    • Saxena, Jyotisna; Tanner, Ralph S.
    • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 4
    • DOI: 10.1007/s10295-010-0794-6