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Title: Engineering Clostridium for improved solvent production: recent progress and perspective

Abstract

Clostridia are Gram-positive, spore-forming, obligate anaerobic bacteria that can produce solvents such as acetone, ethanol, and butanol, which can be used as biofuels or building-block chemicals. Many successful attempts have been made to improve solvent yield and titer from sugars through metabolic engineering of solventogenic and acidogenic clostridia. More recently, cellulolytic and acetogenic clostridia have also attracted high interests for their ability to utilize low-cost renewable substrates such as cellulose and syngas. Process engineering such as in situ butanol recovery and consolidated bioprocessing (CBP) has been developed for improved solvent titer and productivity. This review focuses on metabolic and process engineering strategies for solvent production from sugars, lignocellulosic biomass, and syngas by various clostridia, including conventional solventogenic Clostridium acetobutylicum, engineered acidogens such as C. tyrobutyricum and C. cellulovorans, and carboxydotrophic acetogens such as C. carboxidivorans and C. ljungdahlii.

Authors:
 [1];  [2]; ORCiD logo [2]
  1. Dalian Univ. of Technology, Dalian (China); The Ohio State Univ., Columbus, OH (United States)
  2. The Ohio State Univ., Columbus, OH (United States)
Publication Date:
Research Org.:
The Ohio State Univ., Columbus, OH (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1572190
Grant/Contract Number:  
EE0007005
Resource Type:
Accepted Manuscript
Journal Name:
Applied Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: 103; Journal Issue: 14; Journal ID: ISSN 0175-7598
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Biofuels; butanol; solvent; clostridia; fermentation; metabolic engineering

Citation Formats

Cheng, Chi, Bao, Teng, and Yang, Shang-Tian. Engineering Clostridium for improved solvent production: recent progress and perspective. United States: N. p., 2019. Web. doi:10.1007/s00253-019-09916-7.
Cheng, Chi, Bao, Teng, & Yang, Shang-Tian. Engineering Clostridium for improved solvent production: recent progress and perspective. United States. doi:10.1007/s00253-019-09916-7.
Cheng, Chi, Bao, Teng, and Yang, Shang-Tian. Wed . "Engineering Clostridium for improved solvent production: recent progress and perspective". United States. doi:10.1007/s00253-019-09916-7.
@article{osti_1572190,
title = {Engineering Clostridium for improved solvent production: recent progress and perspective},
author = {Cheng, Chi and Bao, Teng and Yang, Shang-Tian},
abstractNote = {Clostridia are Gram-positive, spore-forming, obligate anaerobic bacteria that can produce solvents such as acetone, ethanol, and butanol, which can be used as biofuels or building-block chemicals. Many successful attempts have been made to improve solvent yield and titer from sugars through metabolic engineering of solventogenic and acidogenic clostridia. More recently, cellulolytic and acetogenic clostridia have also attracted high interests for their ability to utilize low-cost renewable substrates such as cellulose and syngas. Process engineering such as in situ butanol recovery and consolidated bioprocessing (CBP) has been developed for improved solvent titer and productivity. This review focuses on metabolic and process engineering strategies for solvent production from sugars, lignocellulosic biomass, and syngas by various clostridia, including conventional solventogenic Clostridium acetobutylicum, engineered acidogens such as C. tyrobutyricum and C. cellulovorans, and carboxydotrophic acetogens such as C. carboxidivorans and C. ljungdahlii.},
doi = {10.1007/s00253-019-09916-7},
journal = {Applied Microbiology and Biotechnology},
number = 14,
volume = 103,
place = {United States},
year = {2019},
month = {5}
}

Journal Article:
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This content will become publicly available on May 29, 2020
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Works referenced in this record:

Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
journal, January 2013


PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum
journal, May 2008


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journal, December 1993


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Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
journal, January 2009

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    Works referencing / citing this record:

    Aldehyde-alcohol dehydrogenase and/or thiolase overexpression coupled with CoA transferase downregulation lead to higher alcohol titers and selectivity in Clostridium acetobutylicum fermentations
    journal, January 2009

    • Sillers, Ryan; Al-Hinai, Mohab Ali; Papoutsakis, Eleftherios T.
    • Biotechnology and Bioengineering, Vol. 102, Issue 1, p. 38-49
    • DOI: 10.1002/bit.22058

    Biological conversion of coal and coal-derived synthesis gas
    journal, December 1993


    Recent progress in consolidated bioprocessing
    journal, June 2012

    • Olson, Daniel G.; McBride, John E.; Joe Shaw, A.
    • Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405
    • DOI: 10.1016/j.copbio.2011.11.026

    Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum
    journal, January 2013


    PerR acts as a switch for oxygen tolerance in the strict anaerobe Clostridium acetobutylicum
    journal, May 2008