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Title: Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose

Abstract

Clostridium carboxidivorans can convert CO 2, CO and H 2 to ethanol and n-butanol; however, its industrial application is limited by the lack of tools for metabolic pathway engineering. In this study, C. carboxidivorans was successfully engineered to overexpress aor, adhE2, and fnr together with adhE2 or aor. In glucose fermentation, all engineered strains showed higher alcohol yields compared to the wild-type. Strains overexpressing aor showed CO 2 re-assimilation during heterotrophic growth. In syngas fermentation, compared to the wild-type, the strain overexpressing adhE2 produced ~50% more ethanol and the strain overexpressing adhE2 and fnr produced ~18% more butanol and ~22% more ethanol. Interestingly, both strains showed obvious acid re-assimilation, with <0.15 g/L total acid remaining at the end of fermentation. Overexpressing fnr with adhE2 enhanced butanol production compared to only adhE2. Furthermore, this is the first report of overexpressing homologous and heterologous genes in C. carboxidivorans for enhancing alcohols production from syngas and glucose.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [3]; ORCiD logo [3]
  1. Dalian Univ. of Technology, Dalian (China); The Ohio State Univ., Columbus, OH (United States)
  2. The Ohio State Univ., Columbus, OH (United States); Harbin Inst. of Technology, Harbin (China)
  3. The Ohio State Univ., Columbus, OH (United States)
Publication Date:
Research Org.:
The Ohio State Univ., Columbus, OH (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1572181
Alternate Identifier(s):
OSTI ID: 1548095
Grant/Contract Number:  
EE0007005
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 284; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Aldehyde/alcohol dehydrogenase; Aldehyde:ferredoxin oxidoreductase; Clostridium carboxidivorans; Ferredoxin-NAD+ reductase; Syngas fermentation; Metabolic engineering

Citation Formats

Cheng, Chi, Li, Weiming, Lin, Meng, and Yang, Shang-Tian. Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose. United States: N. p., 2019. Web. doi:10.1016/j.biortech.2019.03.145.
Cheng, Chi, Li, Weiming, Lin, Meng, & Yang, Shang-Tian. Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose. United States. doi:10.1016/j.biortech.2019.03.145.
Cheng, Chi, Li, Weiming, Lin, Meng, and Yang, Shang-Tian. Sat . "Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose". United States. doi:10.1016/j.biortech.2019.03.145.
@article{osti_1572181,
title = {Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose},
author = {Cheng, Chi and Li, Weiming and Lin, Meng and Yang, Shang-Tian},
abstractNote = {Clostridium carboxidivorans can convert CO2, CO and H2 to ethanol and n-butanol; however, its industrial application is limited by the lack of tools for metabolic pathway engineering. In this study, C. carboxidivorans was successfully engineered to overexpress aor, adhE2, and fnr together with adhE2 or aor. In glucose fermentation, all engineered strains showed higher alcohol yields compared to the wild-type. Strains overexpressing aor showed CO2 re-assimilation during heterotrophic growth. In syngas fermentation, compared to the wild-type, the strain overexpressing adhE2 produced ~50% more ethanol and the strain overexpressing adhE2 and fnr produced ~18% more butanol and ~22% more ethanol. Interestingly, both strains showed obvious acid re-assimilation, with <0.15 g/L total acid remaining at the end of fermentation. Overexpressing fnr with adhE2 enhanced butanol production compared to only adhE2. Furthermore, this is the first report of overexpressing homologous and heterologous genes in C. carboxidivorans for enhancing alcohols production from syngas and glucose.},
doi = {10.1016/j.biortech.2019.03.145},
journal = {Bioresource Technology},
number = C,
volume = 284,
place = {United States},
year = {2019},
month = {3}
}

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This content will become publicly available on March 30, 2020
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Cited by: 5 works
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Works referencing / citing this record:

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

  • Cheng, Chi; Bao, Teng; Yang, Shang-Tian
  • Applied Microbiology and Biotechnology, Vol. 103, Issue 14
  • DOI: 10.1007/s00253-019-09916-7