Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose

doi:10.1016/j.biortech.2019.03.145

Title: Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose

Abstract

Clostridium carboxidivorans can convert CO₂, CO and H₂ to ethanol and n-butanol; however, its industrial application is limited by the lack of tools for metabolic pathway engineering. In this study, C. carboxidivorans was successfully engineered to overexpress aor, adhE2, and fnr together with adhE2 or aor. In glucose fermentation, all engineered strains showed higher alcohol yields compared to the wild-type. Strains overexpressing aor showed CO₂ re-assimilation during heterotrophic growth. In syngas fermentation, compared to the wild-type, the strain overexpressing adhE2 produced ~50% more ethanol and the strain overexpressing adhE2 and fnr produced ~18% more butanol and ~22% more ethanol. Interestingly, both strains showed obvious acid re-assimilation, with <0.15 g/L total acid remaining at the end of fermentation. Overexpressing fnr with adhE2 enhanced butanol production compared to only adhE2. Furthermore, this is the first report of overexpressing homologous and heterologous genes in C. carboxidivorans for enhancing alcohols production from syngas and glucose.

Authors:

^[1];

^[2]; Lin, Meng ^[3];

^[3]

Dalian Univ. of Technology, Dalian (China); The Ohio State Univ., Columbus, OH (United States)
The Ohio State Univ., Columbus, OH (United States); Harbin Inst. of Technology, Harbin (China)
The Ohio State Univ., Columbus, OH (United States)

Publication Date:: 2019-03-30

Research Org.:: The Ohio State Univ., Columbus, OH (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

OSTI Identifier:: 1572181

Alternate Identifier(s):: OSTI ID: 1548095

Grant/Contract Number:: EE0007005

Resource Type:: Accepted Manuscript

Journal Name:: Bioresource Technology

Additional Journal Information:: Journal Volume: 284; Journal Issue: C; Journal ID: ISSN 0960-8524

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 09 BIOMASS FUELS; Aldehyde/alcohol dehydrogenase; Aldehyde:ferredoxin oxidoreductase; Clostridium carboxidivorans; Ferredoxin-NAD+ reductase; Syngas fermentation; Metabolic engineering

Citation Formats


                    Cheng, Chi, Li, Weiming, Lin, Meng, and Yang, Shang-Tian. Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose.  United States: N. p., 2019. 
        Web.  doi:10.1016/j.biortech.2019.03.145.

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                    Cheng, Chi, Li, Weiming, Lin, Meng, & Yang, Shang-Tian. Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose.  United States.  doi:10.1016/j.biortech.2019.03.145.

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                    Cheng, Chi, Li, Weiming, Lin, Meng, and Yang, Shang-Tian. Sat .  
        "Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose".  United States.  doi:10.1016/j.biortech.2019.03.145.  https://www.osti.gov/servlets/purl/1572181.

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@article{osti_1572181,

  title        = {Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose},

  author       = {Cheng, Chi and Li, Weiming and Lin, Meng and Yang, Shang-Tian},

  abstractNote = {Clostridium carboxidivorans can convert CO2, CO and H2 to ethanol and n-butanol; however, its industrial application is limited by the lack of tools for metabolic pathway engineering. In this study, C. carboxidivorans was successfully engineered to overexpress aor, adhE2, and fnr together with adhE2 or aor. In glucose fermentation, all engineered strains showed higher alcohol yields compared to the wild-type. Strains overexpressing aor showed CO2 re-assimilation during heterotrophic growth. In syngas fermentation, compared to the wild-type, the strain overexpressing adhE2 produced ~50% more ethanol and the strain overexpressing adhE2 and fnr produced ~18% more butanol and ~22% more ethanol. Interestingly, both strains showed obvious acid re-assimilation, with <0.15 g/L total acid remaining at the end of fermentation. Overexpressing fnr with adhE2 enhanced butanol production compared to only adhE2. Furthermore, this is the first report of overexpressing homologous and heterologous genes in C. carboxidivorans for enhancing alcohols production from syngas and glucose.},

  doi          = {10.1016/j.biortech.2019.03.145},

  journal      = {Bioresource Technology},

  number       = C,

  volume       = 284,

  place        = {United States},

  year         = {2019},

  month        = {3}

}

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DOI: 10.1016/j.biortech.2019.03.145

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Works referencing / citing this record:

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

Cheng, Chi; Bao, Teng; Yang, Shang-Tian
Applied Microbiology and Biotechnology, Vol. 103, Issue 14
DOI: 10.1007/s00253-019-09916-7

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

Cheng, Chi; Bao, Teng; Yang, Shang-Tian
Applied Microbiology and Biotechnology, Vol. 103, Issue 14
DOI: 10.1007/s00253-019-09916-7

Metabolic engineering of microorganisms for the production of ethanol and butanol from oxides of carbon
journal, August 2019

Woo, Ji Eun; Jang, Yu-Sin
Applied Microbiology and Biotechnology, Vol. 103, Issue 20
DOI: 10.1007/s00253-019-10072-1

Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans
journal, January 2020

Benevenuti, Carolina; Botelho, Alanna; Ribeiro, Roberta
Catalysts, Vol. 10, Issue 1
DOI: 10.3390/catal10010059

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Title: Metabolic engineering of Clostridium carboxidivorans for enhanced ethanol and butanol production from syngas and glucose

Abstract

Citation Formats

Engineering Clostridium for improved solvent production: recent progress and perspective journal, May 2019

Engineering Clostridium for improved solvent production: recent progress and perspective journal, May 2019

Metabolic engineering of microorganisms for the production of ethanol and butanol from oxides of carbon journal, August 2019

Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans journal, January 2020

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

Metabolic engineering of microorganisms for the production of ethanol and butanol from oxides of carbon
journal, August 2019

Experimental Design to Improve Cell Growth and Ethanol Production in Syngas Fermentation by Clostridium carboxidivorans
journal, January 2020