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Title: n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases

Abstract

With high cellulolytic and acetic/butyric acids production abilities, Clostridium cellulovorans is promising for use to produce cellulosic n-butanol. Here, we introduced three different aldehyde/alcohol dehydrogenases encoded by bdhB, adhE1, and adhE2 from Clostridium acetobutylicum into C. cellulovorans and studied their effects on ethanol and n-butanol production. Compared to AdhE2, AdhE1 was more specific for n-butanol biosynthesis over ethanol. Co-expressing adhE1 with bdhB produced a comparable amount of butanol but significantly less ethanol, leading to a high butanol/ethanol ratio of 7.0 and 5.6 (g/g) in glucose and cellulose fermentation, respectively. Co-expressing adhE1 or adhE2 with bdhB did not increase butanol production because the activity of BdhB was limited by the NADPH availability in C. cellulovorans. Altogether, the strain overexpressing adhE2 alone produced the most n-butanol (4.0 g/L, yield: 0.22 ± 0.01 g/g). Based on the insights from this study, further metabolic engineering of C. cellulovorans for cellulosic n-butanol production is suggested.

Authors:
 [1];  [1];  [2];  [3]; ORCiD logo [1]
  1. The Ohio State Univ., Columbus, OH (United States)
  2. The Ohio State Univ., Columbus, OH (United States); Hebei Univ. of Economics & Business, Shijiazhuang (People's Republic of China)
  3. The Ohio State Univ., Columbus, OH (United States); Changchun Univ. of Technology, Changchun (People's Republic of China)
Publication Date:
Research Org.:
The Ohio State Univ., Columbus, OH (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1572179
Alternate Identifier(s):
OSTI ID: 1636742
Grant/Contract Number:  
EE0007005
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 285; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Biofuel; Clostridium cellulovorans; Consolidated bioprocessing; Metabolic engineering; Butyraldehyde dehydrogenase; Butanol dehydrogenase

Citation Formats

Bao, Teng, Zhao, Jingbo, Li, Jing, Liu, Xin, and Yang, Shang-Tian. n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases. United States: N. p., 2019. Web. doi:10.1016/j.biortech.2019.121316.
Bao, Teng, Zhao, Jingbo, Li, Jing, Liu, Xin, & Yang, Shang-Tian. n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases. United States. https://doi.org/10.1016/j.biortech.2019.121316
Bao, Teng, Zhao, Jingbo, Li, Jing, Liu, Xin, and Yang, Shang-Tian. Wed . "n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases". United States. https://doi.org/10.1016/j.biortech.2019.121316. https://www.osti.gov/servlets/purl/1572179.
@article{osti_1572179,
title = {n-Butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases},
author = {Bao, Teng and Zhao, Jingbo and Li, Jing and Liu, Xin and Yang, Shang-Tian},
abstractNote = {With high cellulolytic and acetic/butyric acids production abilities, Clostridium cellulovorans is promising for use to produce cellulosic n-butanol. Here, we introduced three different aldehyde/alcohol dehydrogenases encoded by bdhB, adhE1, and adhE2 from Clostridium acetobutylicum into C. cellulovorans and studied their effects on ethanol and n-butanol production. Compared to AdhE2, AdhE1 was more specific for n-butanol biosynthesis over ethanol. Co-expressing adhE1 with bdhB produced a comparable amount of butanol but significantly less ethanol, leading to a high butanol/ethanol ratio of 7.0 and 5.6 (g/g) in glucose and cellulose fermentation, respectively. Co-expressing adhE1 or adhE2 with bdhB did not increase butanol production because the activity of BdhB was limited by the NADPH availability in C. cellulovorans. Altogether, the strain overexpressing adhE2 alone produced the most n-butanol (4.0 g/L, yield: 0.22 ± 0.01 g/g). Based on the insights from this study, further metabolic engineering of C. cellulovorans for cellulosic n-butanol production is suggested.},
doi = {10.1016/j.biortech.2019.121316},
journal = {Bioresource Technology},
number = C,
volume = 285,
place = {United States},
year = {2019},
month = {4}
}

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