Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies

Bao, Teng; Cheng, Chi; Xin, Xin; Wang, Jufang; Wang, Mingqi; Yang, Shang-Tian

doi:10.1016/j.ygeno.2018.11.020

Title: Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies

Journal Article · 20 November 2018 · Genomics

DOI: https://doi.org/10.1016/j.ygeno.2018.11.020 · OSTI ID:1572178

Bao, Teng ^[1];

^[2]; Xin, Xin ^[2]; Wang, Jufang ^[3]; Wang, Mingqi ^[4];

^[2]

The Ohio State Univ., Columbus, OH (United States); The Ohio State University
The Ohio State Univ., Columbus, OH (United States)
South China Univ. of Technology, Guangzhou (China)
Zhejiang Univ., Zhejiang (China)

Clostridium formicoaceticum, a Gram-negative mixotrophic homoacetogen, produces acetic acid as the sole metabolic product from various carbon sources, including fructose, glycerol, formate, and CO₂. Its genome of 4.59-Mbp contains a highly conserved Wood-Ljungdahl pathway gene cluster with the same layout as that in other mixotrophic acetogens, including Clostridium aceticum, Clostridium carboxidivorans, and Clostridium ljungdahlii. For energy conservation, C. formicoaceticum does not have all the genes required for the synthesis of cytochrome or quinone used for generating proton gradient in H⁺-dependent acetogens such as Moorella thermoacetica; instead, it has the Rnf system and a Na⁺-translocating ATPase similar to the one in Acetobacterium woodii. Furthermore its growth in both heterotrophic and autotrophic media were dependent on the sodium concentration. C. formicoaceticum has genes encoding acetaldehyde dehydrogenases, alcohol dehydrogenases, and aldehyde oxidoreductases, which could convert acetyl-CoA and acetate to ethanol and butyrate to butanol under excessive reducing equivalent conditions.

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Research Organization:: The Ohio State Univ., Columbus, OH (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Grant/Contract Number:: EE0007005

OSTI ID:: 1572178

Journal Information:: Genomics, Journal Name: Genomics Journal Issue: 6 Vol. 111; ISSN 0888-7543

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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