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Title: Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies

Abstract

Clostridium formicoaceticum, a Gram-negative mixotrophic homoacetogen, produces acetic acid as the sole metabolic product from various carbon sources, including fructose, glycerol, formate, and CO2. Its genome of 4.59-Mbp contains a highly conserved Wood-Ljungdahl pathway gene cluster with the same layout as that in other mixotrophic acetogens, including Clostridium aceticum, Clostridium carboxidivorans, and Clostridium ljungdahlii. For energy conservation, C. formicoaceticum does not have all the genes required for the synthesis of cytochrome or quinone used for generating proton gradient in H+-dependent acetogens such as Moorella thermoacetica; instead, it has the Rnf system and a Na+-translocating ATPase similar to the one in Acetobacterium woodii. Furthermore its growth in both heterotrophic and autotrophic media were dependent on the sodium concentration. C. formicoaceticum has genes encoding acetaldehyde dehydrogenases, alcohol dehydrogenases, and aldehyde oxidoreductases, which could convert acetyl-CoA and acetate to ethanol and butyrate to butanol under excessive reducing equivalent conditions.

Authors:
 [1]; ORCiD logo [1];  [1];  [2];  [3]; ORCiD logo [1]
  1. The Ohio State Univ., Columbus, OH (United States)
  2. South China Univ. of Technology, Guangzhou (China)
  3. Zhejiang Univ., Zhejiang (China)
Publication Date:
Research Org.:
The Ohio State Univ., Columbus, OH (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
OSTI Identifier:
1572178
Grant/Contract Number:  
EE0007005
Resource Type:
Accepted Manuscript
Journal Name:
Genomics
Additional Journal Information:
Journal Volume: 111; Journal Issue: 6; Journal ID: ISSN 0888-7543
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Acetate; Acetogen; Clostridium formicoaceticum; Genomic analysis; Mixotrophy; Wood-Ljungdahl pathway

Citation Formats

Bao, Teng, Cheng, Chi, Xin, Xin, Wang, Jufang, Wang, Mingqi, and Yang, Shang-Tian. Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies. United States: N. p., 2018. Web. doi:10.1016/j.ygeno.2018.11.020.
Bao, Teng, Cheng, Chi, Xin, Xin, Wang, Jufang, Wang, Mingqi, & Yang, Shang-Tian. Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies. United States. doi:10.1016/j.ygeno.2018.11.020.
Bao, Teng, Cheng, Chi, Xin, Xin, Wang, Jufang, Wang, Mingqi, and Yang, Shang-Tian. Tue . "Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies". United States. doi:10.1016/j.ygeno.2018.11.020. https://www.osti.gov/servlets/purl/1572178.
@article{osti_1572178,
title = {Deciphering mixotrophic Clostridium formicoaceticum metabolism and energy conservation: Genomic analysis and experimental studies},
author = {Bao, Teng and Cheng, Chi and Xin, Xin and Wang, Jufang and Wang, Mingqi and Yang, Shang-Tian},
abstractNote = {Clostridium formicoaceticum, a Gram-negative mixotrophic homoacetogen, produces acetic acid as the sole metabolic product from various carbon sources, including fructose, glycerol, formate, and CO2. Its genome of 4.59-Mbp contains a highly conserved Wood-Ljungdahl pathway gene cluster with the same layout as that in other mixotrophic acetogens, including Clostridium aceticum, Clostridium carboxidivorans, and Clostridium ljungdahlii. For energy conservation, C. formicoaceticum does not have all the genes required for the synthesis of cytochrome or quinone used for generating proton gradient in H+-dependent acetogens such as Moorella thermoacetica; instead, it has the Rnf system and a Na+-translocating ATPase similar to the one in Acetobacterium woodii. Furthermore its growth in both heterotrophic and autotrophic media were dependent on the sodium concentration. C. formicoaceticum has genes encoding acetaldehyde dehydrogenases, alcohol dehydrogenases, and aldehyde oxidoreductases, which could convert acetyl-CoA and acetate to ethanol and butyrate to butanol under excessive reducing equivalent conditions.},
doi = {10.1016/j.ygeno.2018.11.020},
journal = {Genomics},
number = 6,
volume = 111,
place = {United States},
year = {2018},
month = {11}
}

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Works referencing / citing this record:

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

  • Cheng, Chi; Bao, Teng; Yang, Shang-Tian
  • Applied Microbiology and Biotechnology, Vol. 103, Issue 14
  • DOI: 10.1007/s00253-019-09916-7

Engineering Clostridium for improved solvent production: recent progress and perspective
journal, May 2019

  • Cheng, Chi; Bao, Teng; Yang, Shang-Tian
  • Applied Microbiology and Biotechnology, Vol. 103, Issue 14
  • DOI: 10.1007/s00253-019-09916-7