Xylose Assimilation Enhances Production of Isobutanol in Engineered Saccharomyces cerevisiae

Lane, Stephan; Zhang, Yanfei; Yun, Eun Ju; Ziolkowski, Leah; Zhang, Guochang; Jin, Yong‐Su; Avalos, José L.

doi:10.1002/bit.27202

Title: Xylose Assimilation Enhances Production of Isobutanol in Engineered Saccharomyces cerevisiae

Journal Article · 20 October 2019 · Biotechnology and Bioengineering

DOI: https://doi.org/10.1002/bit.27202 · OSTI ID:1572071

Lane, Stephan ^[1]; Zhang, Yanfei ^[2]; Yun, Eun Ju ^[3]; Ziolkowski, Leah ^[3]; Zhang, Guochang ^[3];

^[3]; Avalos, José L. ^[4]

Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); CABBI
Princeton Univ., Princeton, NJ (United States)
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
Princeton Univ., Princeton, NJ (United States); Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)

Bioconversion of xylose—the second most abundant sugar in nature—into high–value fuels and chemicals by engineered Saccharomyces cerevisiae has been a long–term goal of the metabolic engineering community. Although most efforts have heavily focused on the production of ethanol by engineered S. cerevisiae, yields and productivities of ethanol produced from xylose have remained inferior as compared to ethanol produced from glucose. However, this entrenched focus on ethanol has concealed the fact that many aspects of xylose metabolism favor the production of non–ethanol products. Through reduced overall metabolic flux, a more respiratory nature of consumption, and evading glucose signaling pathways, the bioconversion of xylose can be more amenable to redirecting flux away from ethanol towards a desired target product. In this report, we show that coupling xylose consumption via the oxidoreductive pathway with a mitochondrially–targeted isobutanol biosynthesis pathway leads to enhanced product yields and titers as compared to cultures utilizing glucose or galactose as a carbon source. Through optimization of culture conditions, we achieve 2.6 g/L of isobutanol in fed–batch flask and bioreactor fermentations. Furthermore, these results suggest that there may be synergistic benefits of coupling xylose assimilation with the production of non–ethanol value–added products.

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Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: SC0018420

OSTI ID:: 1572071

Journal Information:: Biotechnology and Bioengineering, Journal Name: Biotechnology and Bioengineering Journal Issue: 2 Vol. 117; ISSN 0006-3592

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
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Title: Xylose Assimilation Enhances Production of Isobutanol in Engineered Saccharomyces cerevisiae

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