Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ
Abstract
Myoepithelial cells play key roles in normal mammary gland development and in limiting pre-invasive to invasive breast tumor progression, yet their differentiation and perturbation in ductal carcinoma in situ (DCIS) are poorly understood. Here, we investigated myoepithelial cells in normal breast tissues of BRCA1 and BRCA2 germline mutation carriers and in non-carrier controls, and in sporadic DCIS. We found that in the normal breast of non-carriers, myoepithelial cells frequently co-express the p63 and TCF7 transcription factors and that p63 and TCF7 show overlapping chromatin peaks associated with differentiated myoepithelium-specific genes. In contrast, in normal breast tissues of BRCA1 mutation carriers the frequency of p63+TCF7+ myoepithelial cells is significantly decreased and p63 and TCF7 chromatin peaks do not overlap. These myoepithelial perturbations in normal breast tissues of BRCA1 germline mutation carriers may play a role in their higher risk of breast cancer. The fraction of p63+TCF7+ myoepithelial cells is also significantly decreased in DCIS, which may be associated with invasive progression.
- Authors:
-
more »
- Harvard Medical School, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Deciphera Pharmaceuticals, Waltham, MA (United States); Harvard Medical School, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Harvard Medical School, Boston, MA (United States)
- Nagoya Univ. Graduate School of Medicine (Japan); Harvard Medical School, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Dana-Farber Cancer Inst., Boston, MA (United States)
- ImmunoGen, Inc., Waltham, MA (United States); Harvard Medical School, Boston, MA (United States)
- EMEA Site Intelligence and Activation, Tel Aviv (Israel); Dana-Farber Cancer Inst., Boston, MA (United States)
- Harvard Medical School, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- WuXi NextCODE, Cambridge, MA (United States); Harvard Medical School, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Second Military Medical Univ., Shanghai (China); Yale Univ., New Haven, CT (United States)
- Univ. of New Mexico School of Medicine, Albuquerque, NM (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Johns Hopkins Univ., Baltimore, MD (United States). School of Medicine
- Metamark Genetics, Inc., Worcester, MA (United States); Harvard Medical School, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Baylor-Charles A. Sammons Cancer Center, Dallas, TX (United States)
- Univ. of California San Francisco Helen Diller Family Comprehensive Cancer Center, CA (United States)
- Johns Hopkins Medical Institutions, Baltimore, MD (United States); Brigham and Women's Hospital (Harvard Medical School), Boston, MA (United States)
- Brigham and Women's Hospital (Harvard Medical School), Boston, MA (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
- Sutter Roseville Medical Center, Roseville, CA (United States)
- Cancer Treatment Centers of America, Atlanta, GA (United States); Sutter Roseville Medical Center, Roseville, CA (United States)
- Univ. of Michigan, Ann Arbor, MI (United States)
- Duke Univ., Durham, NC (United States); Univ. of California San Francisco Helen Diller Family Comprehensive Cancer Center, CA (United States)
- Harvard Univ., Cambridge, MA (United States); Harvard T. H. Chan School of Public Health, Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Yale Univ., New Haven, CT (United States)
- Seoul National Univ. College of Medicine (Korea, Republic of)
- Brigham and Women's Hospital (Harvard Medical School), Boston, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- MD Anderson Cancer Center, Houston, TX (United States)
- Harvard Stem Cell Inst., Cambridge, MA (United States); Dana-Farber Cancer Inst., Boston, MA (United States); Brigham and Women's Hospital (Harvard Medical School), Boston, MA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1572055
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Ding, Lina, Su, Ying, Fassl, Anne, Hinohara, Kunihiko, Qiu, Xintao, Harper, Nicholas W., Huh, Sung Jin, Bloushtain-Qimron, Noga, Jovanović, Bojana, Ekram, Muhammad, Zi, Xiaoyuan, Hines, William C., Alečković, Maša, Caulfield, Ryan J., Bonal, Dennis M., Nguyen, Quang-De, Merino, Vanessa F., Choudhury, Sibgat, Ethington, Gabrielle, Panos, Laura, Grant, Michael, Herlihy, William, Au, Alfred, Rosson, Gedge D., Argani, Pedram, Richardson, Andrea L., Dillon, Deborah, Allred, D. Craig, Babski, Kirsten, Kim, Elizabeth Min Hui, McDonnell, Charles H., Wagner, Jon, Rowberry, Ron, Bobolis, Kristie, Kleer, Celina G., Hwang, E. Shelley, Blum, Joanne L., Cristea, Simona, Sicinski, Piotr, Fan, Rong, Long, Henry W., Sukumar, Saraswati, Park, So Yeon, Garber, Judy E., Bissell, Mina, Yao, Jun, Polyak, Kornelia, and del Alcazar, Carlos Gil. Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. United States: N. p., 2019.
Web. doi:10.1038/s41467-019-12125-5.
Ding, Lina, Su, Ying, Fassl, Anne, Hinohara, Kunihiko, Qiu, Xintao, Harper, Nicholas W., Huh, Sung Jin, Bloushtain-Qimron, Noga, Jovanović, Bojana, Ekram, Muhammad, Zi, Xiaoyuan, Hines, William C., Alečković, Maša, Caulfield, Ryan J., Bonal, Dennis M., Nguyen, Quang-De, Merino, Vanessa F., Choudhury, Sibgat, Ethington, Gabrielle, Panos, Laura, Grant, Michael, Herlihy, William, Au, Alfred, Rosson, Gedge D., Argani, Pedram, Richardson, Andrea L., Dillon, Deborah, Allred, D. Craig, Babski, Kirsten, Kim, Elizabeth Min Hui, McDonnell, Charles H., Wagner, Jon, Rowberry, Ron, Bobolis, Kristie, Kleer, Celina G., Hwang, E. Shelley, Blum, Joanne L., Cristea, Simona, Sicinski, Piotr, Fan, Rong, Long, Henry W., Sukumar, Saraswati, Park, So Yeon, Garber, Judy E., Bissell, Mina, Yao, Jun, Polyak, Kornelia, & del Alcazar, Carlos Gil. Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ. United States. doi:10.1038/s41467-019-12125-5.
Ding, Lina, Su, Ying, Fassl, Anne, Hinohara, Kunihiko, Qiu, Xintao, Harper, Nicholas W., Huh, Sung Jin, Bloushtain-Qimron, Noga, Jovanović, Bojana, Ekram, Muhammad, Zi, Xiaoyuan, Hines, William C., Alečković, Maša, Caulfield, Ryan J., Bonal, Dennis M., Nguyen, Quang-De, Merino, Vanessa F., Choudhury, Sibgat, Ethington, Gabrielle, Panos, Laura, Grant, Michael, Herlihy, William, Au, Alfred, Rosson, Gedge D., Argani, Pedram, Richardson, Andrea L., Dillon, Deborah, Allred, D. Craig, Babski, Kirsten, Kim, Elizabeth Min Hui, McDonnell, Charles H., Wagner, Jon, Rowberry, Ron, Bobolis, Kristie, Kleer, Celina G., Hwang, E. Shelley, Blum, Joanne L., Cristea, Simona, Sicinski, Piotr, Fan, Rong, Long, Henry W., Sukumar, Saraswati, Park, So Yeon, Garber, Judy E., Bissell, Mina, Yao, Jun, Polyak, Kornelia, and del Alcazar, Carlos Gil. Fri .
"Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ". United States. doi:10.1038/s41467-019-12125-5. https://www.osti.gov/servlets/purl/1572055.
@article{osti_1572055,
title = {Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situ},
author = {Ding, Lina and Su, Ying and Fassl, Anne and Hinohara, Kunihiko and Qiu, Xintao and Harper, Nicholas W. and Huh, Sung Jin and Bloushtain-Qimron, Noga and Jovanović, Bojana and Ekram, Muhammad and Zi, Xiaoyuan and Hines, William C. and Alečković, Maša and Caulfield, Ryan J. and Bonal, Dennis M. and Nguyen, Quang-De and Merino, Vanessa F. and Choudhury, Sibgat and Ethington, Gabrielle and Panos, Laura and Grant, Michael and Herlihy, William and Au, Alfred and Rosson, Gedge D. and Argani, Pedram and Richardson, Andrea L. and Dillon, Deborah and Allred, D. Craig and Babski, Kirsten and Kim, Elizabeth Min Hui and McDonnell, Charles H. and Wagner, Jon and Rowberry, Ron and Bobolis, Kristie and Kleer, Celina G. and Hwang, E. Shelley and Blum, Joanne L. and Cristea, Simona and Sicinski, Piotr and Fan, Rong and Long, Henry W. and Sukumar, Saraswati and Park, So Yeon and Garber, Judy E. and Bissell, Mina and Yao, Jun and Polyak, Kornelia and del Alcazar, Carlos Gil},
abstractNote = {Myoepithelial cells play key roles in normal mammary gland development and in limiting pre-invasive to invasive breast tumor progression, yet their differentiation and perturbation in ductal carcinoma in situ (DCIS) are poorly understood. Here, we investigated myoepithelial cells in normal breast tissues of BRCA1 and BRCA2 germline mutation carriers and in non-carrier controls, and in sporadic DCIS. We found that in the normal breast of non-carriers, myoepithelial cells frequently co-express the p63 and TCF7 transcription factors and that p63 and TCF7 show overlapping chromatin peaks associated with differentiated myoepithelium-specific genes. In contrast, in normal breast tissues of BRCA1 mutation carriers the frequency of p63+TCF7+ myoepithelial cells is significantly decreased and p63 and TCF7 chromatin peaks do not overlap. These myoepithelial perturbations in normal breast tissues of BRCA1 germline mutation carriers may play a role in their higher risk of breast cancer. The fraction of p63+TCF7+ myoepithelial cells is also significantly decreased in DCIS, which may be associated with invasive progression.},
doi = {10.1038/s41467-019-12125-5},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {9}
}
Web of Science
Works referenced in this record:
β4 integrin-dependent formation of polarized three-dimensional architecture confers resistance to apoptosis in normal and malignant mammary epithelium
journal, September 2002
- Weaver, Valerie M.; Lelièvre, Sophie; Lakins, Johnathon N.
- Cancer Cell, Vol. 2, Issue 3
Basal Cell Signaling by p63 Controls Luminal Progenitor Function and Lactation via NRG1
journal, January 2014
- Forster, Nicole; Saladi, Srinivas Vinod; van Bragt, Maaike
- Developmental Cell, Vol. 28, Issue 2
Role of Notch signaling in cell-fate determination of human mammary stem/progenitor cells
journal, August 2004
- Dontu, Gabriela; Jackson, Kyle W.; McNicholas, Erin
- Breast Cancer Research, Vol. 6, Issue 6
In vitro propagation and transcriptional profiling of human mammary stem/progenitor cells
journal, May 2003
- Dontu, G.
- Genes & Development, Vol. 17, Issue 10
Regulation of In Situ to Invasive Breast Carcinoma Transition
journal, May 2008
- Hu, Min; Yao, Jun; Carroll, Danielle K.
- Cancer Cell, Vol. 13, Issue 5
Sustained activation of the HER1-ERK1/2-RSK signaling pathway controls myoepithelial cell fate in human mammary tissue
journal, August 2011
- Pasic, L.; Eisinger-Mathason, T. S. K.; Velayudhan, B. T.
- Genes & Development, Vol. 25, Issue 15
Numb and Numbl act to determine mammary myoepithelial cell fate, maintain epithelial identity, and support lactogenesis
journal, October 2016
- Zhang, Yue; Li, Fengyin; Song, Yongli
- The FASEB Journal, Vol. 30, Issue 10
ΔNp63 promotes stem cell activity in mammary gland development and basal-like breast cancer by enhancing Fzd7 expression and Wnt signalling
journal, September 2014
- Chakrabarti, Rumela; Wei, Yong; Hwang, Julie
- Nature Cell Biology, Vol. 16, Issue 10
The mammary myoepithelial cell - Cinderella or ugly sister?
journal, February 2000
- Lakhani, Sunil R.; O'Hare, Michael J.
- Breast Cancer Research, Vol. 3, Issue 1
The importance of being a myoepithelial cell
journal, December 2002
- Deugnier, Marie-Ange; Teulière, Jérôme; Faraldo, Marisa M.
- Breast Cancer Research, Vol. 4, Issue 6
The myoepithelial defense: a host defense against cancer
journal, January 1997
- Sternlicht, M. D.; Barsky, S. H.
- Medical Hypotheses, Vol. 48, Issue 1
Myoepithelial cells and basal lamina in poorly differentiated in situ duct carcinoma of the breast
journal, March 1999
- Damiani, S.; Ludvikova, M.; Tomasic, G.
- Virchows Archiv, Vol. 434, Issue 3
Phenotypic Alterations in Ductal Carcinoma In Situ-associated Myoepithelial Cells: Biologic and Diagnostic Implications
journal, January 2009
- Hilson, Justin B.; Schnitt, Stuart J.; Collins, Laura C.
- The American Journal of Surgical Pathology, Vol. 33, Issue 2
Neuropilin-1 is differentially expressed in myoepithelial cells and vascular smooth muscle cells in preneoplastic and neoplastic human breast: A possible marker for the progression of breast cancer
journal, September 2002
- Stephenson, John M.; Banerjee, Snigdha; Saxena, Neela K.
- International Journal of Cancer, Vol. 101, Issue 5
Altered Microenvironment Promotes Progression of Preinvasive Breast Cancer: Myoepithelial Expression of v 6 Integrin in DCIS Identifies High-risk Patients and Predicts Recurrence
journal, October 2013
- Allen, M. D.; Thomas, G. J.; Clark, S.
- Clinical Cancer Research, Vol. 20, Issue 2
Myoepithelial Cell Differentiation Markers in Ductal Carcinoma in Situ Progression
journal, November 2015
- Russell, Tanya D.; Jindal, Sonali; Agunbiade, Samiat
- The American Journal of Pathology, Vol. 185, Issue 11
CD10-positive myoepithelial cells are usually prominent around in situ lobular neoplasia of the breast and much less prominent or absent in DCIS
journal, February 2016
- Shousha, Sami; Forbes, Georgina; Hopkins, Ilona
- Journal of Clinical Pathology, Vol. 69, Issue 8
Progression of ductal carcinoma in situ to invasive breast cancer is associated with gene expression programs of EMT and myoepithelia
journal, December 2011
- Knudsen, Erik S.; Ertel, Adam; Davicioni, Elai
- Breast Cancer Research and Treatment, Vol. 133, Issue 3
Low CD10 mRNA Expression Identifies High-Risk Ductal Carcinoma In Situ (DCIS)
journal, August 2010
- Toussaint, Jérôme; Durbecq, Virginie; Altintas, Sevilay
- PLoS ONE, Vol. 5, Issue 8
Morphologic, molecular and microenvironment factors associated with stromal invasion in breast ductal carcinoma in situ: Role of myoepithelial cells
journal, November 2015
- Aguiar, Fernando N.; Cirqueira, Cinthya S.; Bacchi, Carlos E.
- Breast Disease, Vol. 35, Issue 4
Oxytocin enhances myoepithelial cell differentiation and proliferation in the mouse mammary gland.
journal, August 1993
- Sapino, A.; Macrì, L.; Tonda, L.
- Endocrinology, Vol. 133, Issue 2
The CD10 Enzyme Is a Key Player to Identify and Regulate Human Mammary Stem Cells
journal, April 2010
- Bachelard-Cascales, Elodie; Chapellier, Marion; Delay, Emmanuel
- STEM CELLS, Vol. 28, Issue 6
Self-organization is a dynamic and lineage-intrinsic property of mammary epithelial cells
journal, February 2011
- Chanson, L.; Brownfield, D.; Garbe, J. C.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 8
Molecular characterization of the tumor microenvironment in breast cancer
journal, July 2004
- Allinen, Minna; Beroukhim, Rameen; Cai, Li
- Cancer Cell, Vol. 6, Issue 1
Distinct epigenetic changes in the stromal cells of breast cancers
journal, July 2005
- Hu, Min; Yao, Jun; Cai, Li
- Nature Genetics, Vol. 37, Issue 8
Role of COX-2 in epithelial-stromal cell interactions and progression of ductal carcinoma in situ of the breast
journal, February 2009
- Hu, M.; Peluffo, G.; Chen, H.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 9
Immune Escape in Breast Cancer During In Situ to Invasive Carcinoma Transition
journal, June 2017
- Gil Del Alcazar, Carlos R.; Huh, Sung Jin; Ekram, Muhammad B.
- Cancer Discovery, Vol. 7, Issue 10
MCF10DCIS.com Xenograft Model of Human Comedo Ductal Carcinoma In Situ
journal, July 2000
- Miller, Fred R.; Santner, Steven J.; Tait, Larry
- JNCI: Journal of the National Cancer Institute, Vol. 92, Issue 14
Aberrant luminal progenitors as the candidate target population for basal tumor development in BRCA1 mutation carriers
journal, August 2009
- Lim, Elgene; Vaillant, François; Wu, Di
- Nature Medicine, Vol. 15, Issue 8
Genetic Predisposition Directs Breast Cancer Phenotype by Dictating Progenitor Cell Fate
journal, February 2011
- Proia, Theresa A.; Keller, Patricia J.; Gupta, Piyush B.
- Cell Stem Cell, Vol. 8, Issue 2
BRCA1/FANCD2/BRG1-Driven DNA Repair Stabilizes the Differentiation State of Human Mammary Epithelial Cells
journal, July 2016
- Wang, Hua; Bierie, Brian; Li, Andrew G.
- Molecular Cell, Vol. 63, Issue 2
Transcriptome Analysis of the Normal Human Mammary Cell Commitment and Differentiation Process
journal, July 2008
- Raouf, Afshin; Zhao, Yun; To, Karen
- Cell Stem Cell, Vol. 3, Issue 1
A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability
journal, November 2008
- Eirew, Peter; Stingl, John; Raouf, Afshin
- Nature Medicine, Vol. 14, Issue 12
Cell type-specific DNA methylation patterns in the human breast
journal, September 2008
- Bloushtain-Qimron, N.; Yao, J.; Snyder, E. L.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 37
Molecular Profiling of Human Mammary Gland Links Breast Cancer Risk to a p27+ Cell Population with Progenitor Characteristics
journal, July 2013
- Choudhury, Sibgat; Almendro, Vanessa; Merino, Vanessa F.
- Cell Stem Cell, Vol. 13, Issue 1
Expression of Hormone Receptors, Cell Regulatory and Myoepithelial Cell Markers in Lactating Breast and Associated Carcinomas: LETTER TO THE EDITOR
journal, May 2012
- Saglam, Ozlen; Tavassoli, Fattaneh A.
- The Breast Journal, Vol. 18, Issue 4
ALDH1 Is a Marker of Normal and Malignant Human Mammary Stem Cells and a Predictor of Poor Clinical Outcome
journal, November 2007
- Ginestier, Christophe; Hur, Min Hee; Charafe-Jauffret, Emmanuelle
- Cell Stem Cell, Vol. 1, Issue 5
p63 is a p53 homologue required for limb and epidermal morphogenesis
journal, April 1999
- Mills, Alea A.; Zheng, Binhai; Wang, Xiao-Jing
- Nature, Vol. 398, Issue 6729
p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development
journal, April 1999
- Yang, Annie; Schweitzer, Ronen; Sun, Deqin
- Nature, Vol. 398, Issue 6729
Synergy Between Tumor Suppressor APC and the -Catenin-Tcf4 Target Tcf1
journal, September 1999
- Roose, J.
- Science, Vol. 285, Issue 5435
p53/p63/p73 isoforms: an orchestra of isoforms to harmonise cell differentiation and response to stress
journal, April 2006
- Murray-Zmijewski, F.; Lane, D. P.; Bourdon, J-C
- Cell Death & Differentiation, Vol. 13, Issue 6
Diversity of LEF/TCF action in development and disease
journal, December 2006
- Arce, L.; Yokoyama, N. N.; Waterman, M. L.
- Oncogene, Vol. 25, Issue 57
Genetic Changes of Wnt Pathway Genes Are Common Events in Metaplastic Carcinomas of the Breast
journal, July 2008
- Hayes, M. J.; Thomas, D.; Emmons, A.
- Clinical Cancer Research, Vol. 14, Issue 13
p63 in Epithelial Survival, Germ Cell Surveillance, and Neoplasia
journal, January 2010
- Crum, Christopher P.; McKeon, Frank D.
- Annual Review of Pathology: Mechanisms of Disease, Vol. 5, Issue 1
Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes
journal, April 2013
- Whyte, Warren A.; Orlando, David A.; Hnisz, Denes
- Cell, Vol. 153, Issue 2
Models of human core transcriptional regulatory circuitries
journal, February 2016
- Saint-André, Violaine; Federation, Alexander J.; Lin, Charles Y.
- Genome Research, Vol. 26, Issue 3
STRING: a web-server to retrieve and display the repeatedly occurring neighbourhood of a gene
journal, September 2000
- Snel, B.
- Nucleic Acids Research, Vol. 28, Issue 18
Target analysis by integration of transcriptome and ChIP-seq data with BETA
journal, November 2013
- Wang, Su; Sun, Hanfei; Ma, Jian
- Nature Protocols, Vol. 8, Issue 12
p63-Associated Disorders
journal, February 2007
- Rinne, Tuula; Brunner, Hans G.; van Bokhoven, Hans
- Cell Cycle, Vol. 6, Issue 3
Abnormal skin, limb and craniofacial morphogenesis in mice deficient for interferon regulatory factor 6 (Irf6)
journal, October 2006
- Ingraham, Christopher R.; Kinoshita, Akira; Kondo, Shinji
- Nature Genetics, Vol. 38, Issue 11
Regulatory roles of AP-2 transcription factors in vertebrate development, apoptosis and cell-cycle control
journal, December 2000
- Hilger-Eversheim, Kristina; Moser, Markus; Schorle, Hubert
- Gene, Vol. 260, Issue 1-2
Wnt signalling: variety at the core
journal, January 2007
- Hoppler, S.; Kavanagh, C. L.
- Journal of Cell Science, Vol. 120, Issue 3
Genetics of Colonic Cancer
journal, January 1998
- Lynch, Henry T.; Lynch, Jane F.
- Digestion, Vol. 59, Issue 5
Inactivation of Apc perturbs mammary development, but only directly results in acanthoma in the context of Tcf-1 deficiency
journal, September 2002
- Gallagher, Ronald C. J.; Hay, Trevor; Meniel, Valerie
- Oncogene, Vol. 21, Issue 42
A Living Biobank of Breast Cancer Organoids Captures Disease Heterogeneity
journal, January 2018
- Sachs, Norman; de Ligt, Joep; Kopper, Oded
- Cell, Vol. 172, Issue 1-2
Differentially Expressed Genes Regulating the Progression of Ductal Carcinoma In Situ to Invasive Breast Cancer
journal, July 2012
- Lee, S.; Stewart, S.; Nagtegaal, I.
- Cancer Research, Vol. 72, Issue 17
Combined cDNA Array Comparative Genomic Hybridization and Serial Analysis of Gene Expression Analysis of Breast Tumor Progression
journal, April 2006
- Yao, Jun; Weremowicz, Stanislawa; Feng, Bin
- Cancer Research, Vol. 66, Issue 8
KDM5 Histone Demethylase Activity Links Cellular Transcriptomic Heterogeneity to Therapeutic Resistance
journal, December 2018
- Hinohara, Kunihiko; Wu, Hua-Jun; Vigneau, Sébastien
- Cancer Cell, Vol. 34, Issue 6
ChiLin: a comprehensive ChIP-seq and DNase-seq quality control and analysis pipeline
journal, October 2016
- Qin, Qian; Mei, Shenglin; Wu, Qiu
- BMC Bioinformatics, Vol. 17, Issue 1
Fast and accurate short read alignment with Burrows-Wheeler transform
journal, May 2009
- Li, H.; Durbin, R.
- Bioinformatics, Vol. 25, Issue 14
Model-based Analysis of ChIP-Seq (MACS)
journal, January 2008
- Zhang, Yong; Liu, Tao; Meyer, Clifford A.
- Genome Biology, Vol. 9, Issue 9
Simple Combinations of Lineage-Determining Transcription Factors Prime cis-Regulatory Elements Required for Macrophage and B Cell Identities
journal, May 2010
- Heinz, Sven; Benner, Christopher; Spann, Nathanael
- Molecular Cell, Vol. 38, Issue 4
deepTools2: a next generation web server for deep-sequencing data analysis
journal, April 2016
- Ramírez, Fidel; Ryan, Devon P.; Grüning, Björn
- Nucleic Acids Research, Vol. 44, Issue W1
STAR: ultrafast universal RNA-seq aligner
journal, October 2012
- Dobin, Alexander; Davis, Carrie A.; Schlesinger, Felix
- Bioinformatics, Vol. 29, Issue 1
Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
journal, December 2014
- Love, Michael I.; Huber, Wolfgang; Anders, Simon
- Genome Biology, Vol. 15, Issue 12
Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum
journal, May 2011
- Bendall, S. C.; Simonds, E. F.; Qiu, P.
- Science, Vol. 332, Issue 6030
UTX regulates mesoderm differentiation of embryonic stem cells independent of H3K27 demethylase activity
journal, September 2012
- Wang, C.; Lee, J. -E.; Cho, Y. -W.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 38
Works referencing / citing this record:
Loss of myoepithelial calponin‐1 characterizes high‐risk ductal carcinoma in situ cases, which are further stratified by T cell composition
journal, March 2020
- Mitchell, Elizabeth; Jindal, Sonali; Chan, Tiffany
- Molecular Carcinogenesis, Vol. 59, Issue 7