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Title: Broadly protective human antibodies that target the active site of influenza virus neuraminidase

Abstract

Better vaccines against influenza virus are urgently needed to provide broader protection against diverse strains, subtypes, and types. Such efforts are assisted by the identification of novel broadly neutralizing epitopes targeted by protective antibodies. Influenza vaccine development has largely focused on the hemagglutinin, but the other major surface antigen, the neuraminidase, has reemerged as a potential target for universal vaccines. We describe three human monoclonal antibodies isolated from an H3N2-infected donor that bind with exceptional breadth to multiple different influenza A and B virus neuraminidases. These antibodies neutralize the virus, mediate effector functions, are broadly protective in vivo, and inhibit neuraminidase activity by directly binding to the active site. Structural and functional characterization of these antibodies will inform the development of neuraminidase-based universal vaccines against influenza virus.

Authors:
ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo; ORCiD logo; ; ; ORCiD logo; ORCiD logo; ORCiD logo; ; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1571708
Grant/Contract Number:  
AC02-06CH11357; AC02-76SF00515
Resource Type:
Published Article
Journal Name:
Science
Additional Journal Information:
Journal Name: Science Journal Volume: 366 Journal Issue: 6464; Journal ID: ISSN 0036-8075
Publisher:
American Association for the Advancement of Science (AAAS)
Country of Publication:
United States
Language:
English

Citation Formats

Stadlbauer, Daniel, Zhu, Xueyong, McMahon, Meagan, Turner, Jackson S., Wohlbold, Teddy J., Schmitz, Aaron J., Strohmeier, Shirin, Yu, Wenli, Nachbagauer, Raffael, Mudd, Philip A., Wilson, Ian A., Ellebedy, Ali H., and Krammer, Florian. Broadly protective human antibodies that target the active site of influenza virus neuraminidase. United States: N. p., 2019. Web. doi:10.1126/science.aay0678.
Stadlbauer, Daniel, Zhu, Xueyong, McMahon, Meagan, Turner, Jackson S., Wohlbold, Teddy J., Schmitz, Aaron J., Strohmeier, Shirin, Yu, Wenli, Nachbagauer, Raffael, Mudd, Philip A., Wilson, Ian A., Ellebedy, Ali H., & Krammer, Florian. Broadly protective human antibodies that target the active site of influenza virus neuraminidase. United States. doi:10.1126/science.aay0678.
Stadlbauer, Daniel, Zhu, Xueyong, McMahon, Meagan, Turner, Jackson S., Wohlbold, Teddy J., Schmitz, Aaron J., Strohmeier, Shirin, Yu, Wenli, Nachbagauer, Raffael, Mudd, Philip A., Wilson, Ian A., Ellebedy, Ali H., and Krammer, Florian. Thu . "Broadly protective human antibodies that target the active site of influenza virus neuraminidase". United States. doi:10.1126/science.aay0678.
@article{osti_1571708,
title = {Broadly protective human antibodies that target the active site of influenza virus neuraminidase},
author = {Stadlbauer, Daniel and Zhu, Xueyong and McMahon, Meagan and Turner, Jackson S. and Wohlbold, Teddy J. and Schmitz, Aaron J. and Strohmeier, Shirin and Yu, Wenli and Nachbagauer, Raffael and Mudd, Philip A. and Wilson, Ian A. and Ellebedy, Ali H. and Krammer, Florian},
abstractNote = {Better vaccines against influenza virus are urgently needed to provide broader protection against diverse strains, subtypes, and types. Such efforts are assisted by the identification of novel broadly neutralizing epitopes targeted by protective antibodies. Influenza vaccine development has largely focused on the hemagglutinin, but the other major surface antigen, the neuraminidase, has reemerged as a potential target for universal vaccines. We describe three human monoclonal antibodies isolated from an H3N2-infected donor that bind with exceptional breadth to multiple different influenza A and B virus neuraminidases. These antibodies neutralize the virus, mediate effector functions, are broadly protective in vivo, and inhibit neuraminidase activity by directly binding to the active site. Structural and functional characterization of these antibodies will inform the development of neuraminidase-based universal vaccines against influenza virus.},
doi = {10.1126/science.aay0678},
journal = {Science},
number = 6464,
volume = 366,
place = {United States},
year = {2019},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1126/science.aay0678

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Works referenced in this record:

PHENIX: a comprehensive Python-based system for macromolecular structure solution
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