Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol

Nguyen, Anh Duc; Park, Joon Young; Hwang, In Yeub; Hamilton, Richard; Kalyuzhnaya, Marina G.; Kim, Donghyuk; Lee, Eun Yeol

doi:10.1016/j.ymben.2019.10.004

Title: Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol

Full Record
Other Related Research

Authors:: Nguyen, Anh Duc; Park, Joon Young; Hwang, In Yeub; Hamilton, Richard; Kalyuzhnaya, Marina G.; Kim, Donghyuk; Lee, Eun Yeol

Publication Date:: 2020-01-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 1571700

Grant/Contract Number:: FOA DE-FOA-0001085

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Metabolic Engineering

Additional Journal Information:: Journal Name: Metabolic Engineering Journal Volume: 57 Journal Issue: C; Journal ID: ISSN 1096-7176

Publisher:: Elsevier

Country of Publication:: Belgium

Language:: English

Citation Formats


                    Nguyen, Anh Duc, Park, Joon Young, Hwang, In Yeub, Hamilton, Richard, Kalyuzhnaya, Marina G., Kim, Donghyuk, and Lee, Eun Yeol. Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol.  Belgium: N. p., 2020. 
Web.  doi:10.1016/j.ymben.2019.10.004.

Copy to clipboard


                    Nguyen, Anh Duc, Park, Joon Young, Hwang, In Yeub, Hamilton, Richard, Kalyuzhnaya, Marina G., Kim, Donghyuk, & Lee, Eun Yeol. Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol.  Belgium.  https://doi.org/10.1016/j.ymben.2019.10.004

Copy to clipboard


                    Nguyen, Anh Duc, Park, Joon Young, Hwang, In Yeub, Hamilton, Richard, Kalyuzhnaya, Marina G., Kim, Donghyuk, and Lee, Eun Yeol. Wed .  
"Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol".  Belgium.  https://doi.org/10.1016/j.ymben.2019.10.004.

Copy to clipboard


                    
@article{osti_1571700,

  title        = {Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol},

  author       = {Nguyen, Anh Duc and Park, Joon Young and Hwang, In Yeub and Hamilton, Richard and Kalyuzhnaya, Marina G. and Kim, Donghyuk and Lee, Eun Yeol},

  abstractNote = {},

  doi          = {10.1016/j.ymben.2019.10.004},

  journal      = {Metabolic Engineering},

  number       = C,

  volume       = 57,

  place        = {Belgium},

  year         = {2020},

  month        = {1}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (Publisher)

Publisher's Version of Record
https://doi.org/10.1016/j.ymben.2019.10.004

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 6 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

Core Metabolism Shifts during Growth on Methanol versus Methane in the Methanotroph Methylomicrobium buryatense 5GB1

Journal Article Fu, Yanfen ; He, Lian ; Reeve, Jennifer ; ... - mBio

One-carbon compounds such as methane and methanol are of increasing interest as sustainable substrates for biological production of fuels and industrial chemicals. The bacteria that carry out these conversions have been studied for many decades, but gaps exist in our knowledge of their metabolic pathways. One such gap is the difference between growth on methane and growth on methanol. Understanding such metabolism is important, since each has advantages and disadvantages as a feedstock for production of chemicals and fuels. The significance of our research is in the demonstration that the metabolic network is substantially altered in each case and inmore »« less
Cited by 4
https://doi.org/10.1128/mBio.00406-19
Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts

Journal Article Henard, Calvin A. ; Akberdin, Ilya R. ; Kalyuzhnaya, Marina G. ; ... - Green Chemistry

Here, we demonstrate bioconversion of methane to muconic acid, a dicarboxylic acid that can be upgraded to an array of platform chemicals, by three gammaproteobacterial methanotrophs. All engineered methanotrophs expressing a heterologous dihydroxyshikimate dehydratase, protocatechuate decarboxylase, and catechol dioxygenase produced muconic acid from methane, with the highest titer (12.4 mg MA per L), yield (2.8 mg MA per g CH₄), and specific productivity (1.2 mg MA per g dcw, 48 hr) synthesized by Methylotuvimicrobium buryatense, Methylococcus capsulatus, and Methylotuvimicrobium alcaliphilium, respectively. Methylotuvimicrobium alcaliphilum genome-scale model-guided strain engineering predicted that disruption of the pyruvate dehydrogenase or shikimate dehydrogenase would significantly enhancemore »« less
https://doi.org/10.1039/C9GC03722E
Genome Sequence of the Obligate Gammaproteobacterial Methanotroph Methylomicrobium album strain BG8

Journal Article Kits, K. Dimitri ; Kalyuzhnaya, Marina G. ; Klotz, Martin G ; ... - Genome Announcements

The complete genome sequence of Methylomicrobium album BG8, a methane-oxidizing gammaproteobacterium isolated from freshwater, is reported. Aside from conserved inventory for growth on single-carbon compounds, M. album BG8 encodes a range of inventory for additional carbon and nitrogen transformations, but no genes for growth on multi-carbon substrates or for N-fixation.
https://doi.org/10.1128/genomeA.00170-13
Draft Genome Sequences of Gammaproteobacterial Methanotrophs Isolated from Marine Ecosystems

Journal Article Flynn, James D. ; Hirayama, Hisako ; Sakai, Yasuyoshi ; ... - Genome Announcements

The genome sequences ofMethylobacter marinusA45,Methylobactersp. strain BBA5.1, andMethylomarinum vadiIT-4 were obtained. These aerobic methanotrophs are typical members of coastal and hydrothermal vent marine ecosystems.
https://doi.org/10.1128/genomeA.01629-15
Draft Genomes of Gammaproteobacterial Methanotrophs Isolated from Terrestrial Ecosystems: TABLE 1

Journal Article Hamilton, Richard ; Kits, K. Dimitri ; Ramonovskaya, Victoria A. ; ... - Genome Announcements

Genome sequences of Methylobacter luteus, Methylobacter whittenburyi, Methylosarcina fibrata, Methylomicrobium agile, and Methylovulum miyakonense were generated. The strains represent aerobic methanotrophs typically isolated from various terrestrial ecosystems.
https://doi.org/10.1128/genomeA.00515-15

Full Text Available

Similar Records