Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol

doi:10.1016/j.ymben.2019.10.004

This content will become publicly available on October 24, 2020

Title: Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol

Full Record
Other Related Research

Authors:: Nguyen, Anh Duc; Park, Joon Young; Hwang, In Yeub; Hamilton, Richard; Kalyuzhnaya, Marina G.; Kim, Donghyuk; Lee, Eun Yeol

Publication Date:: 2020-01-01

Sponsoring Org.:: USDOE

OSTI Identifier:: 1571700

Grant/Contract Number:: FOA DE-FOA-0001085

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Metabolic Engineering

Additional Journal Information:: Journal Name: Metabolic Engineering Journal Volume: 57 Journal Issue: C; Journal ID: ISSN 1096-7176

Publisher:: Elsevier

Country of Publication:: Belgium

Language:: English

Citation Formats


                    Nguyen, Anh Duc, Park, Joon Young, Hwang, In Yeub, Hamilton, Richard, Kalyuzhnaya, Marina G., Kim, Donghyuk, and Lee, Eun Yeol. Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol.  Belgium: N. p., 2020. 
        Web.  doi:10.1016/j.ymben.2019.10.004.

Copy to clipboard


                    Nguyen, Anh Duc, Park, Joon Young, Hwang, In Yeub, Hamilton, Richard, Kalyuzhnaya, Marina G., Kim, Donghyuk, & Lee, Eun Yeol. Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol.  Belgium.  doi:10.1016/j.ymben.2019.10.004.

Copy to clipboard


                    Nguyen, Anh Duc, Park, Joon Young, Hwang, In Yeub, Hamilton, Richard, Kalyuzhnaya, Marina G., Kim, Donghyuk, and Lee, Eun Yeol. Wed .  
        "Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol".  Belgium.  doi:10.1016/j.ymben.2019.10.004.

Copy to clipboard


                    
@article{osti_1571700,

  title        = {Genome-scale evaluation of core one-carbon metabolism in gammaproteobacterial methanotrophs grown on methane and methanol},

  author       = {Nguyen, Anh Duc and Park, Joon Young and Hwang, In Yeub and Hamilton, Richard and Kalyuzhnaya, Marina G. and Kim, Donghyuk and Lee, Eun Yeol},

  abstractNote = {},

  doi          = {10.1016/j.ymben.2019.10.004},

  journal      = {Metabolic Engineering},

  number       = C,

  volume       = 57,

  place        = {Belgium},

  year         = {2020},

  month        = {1}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

This content will become publicly available on October 24, 2020

Publisher's Version of Record

DOI: 10.1016/j.ymben.2019.10.004

Other availability

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts

Journal Article Henard, Calvin A. ; Akberdin, Ilya R. ; Kalyuzhnaya, Marina G. ; ... - Green Chemistry

Here, we demonstrate bioconversion of methane to muconic acid, a dicarboxylic acid that can be upgraded to an array of platform chemicals, by three gammaproteobacterial methanotrophs. All engineered methanotrophs expressing a heterologous dihydroxyshikimate dehydratase, protocatechuate decarboxylase, and catechol dioxygenase produced muconic acid from methane, with the highest titer (12.4 mg MA per L), yield (2.8 mg MA per g CH ₄), and specific productivity (1.2 mg MA per g dcw, 48 hr) synthesized by Methylotuvimicrobium buryatense, Methylococcus capsulatus, and Methylotuvimicrobium alcaliphilium, respectively. Methylotuvimicrobium alcaliphilum genome-scale model-guided strain engineering predicted that disruption of the pyruvate dehydrogenase or shikimate dehydrogenase would significantlymore »« less
DOI: 10.1039/C9GC03722E
Core Metabolism Shifts during Growth on Methanol versus Methane in the Methanotroph Methylomicrobium buryatense 5GB1

Journal Article Fu, Yanfen ; He, Lian ; Reeve, Jennifer ; ... - mBio (Online)

ABSTRACT Methylomicrobium buryatense 5GB1 is an obligate methylotroph which grows on methane or methanol with similar growth rates. It has long been assumed that the core metabolic pathways must be similar on the two substrates, but recent studies of methane metabolism in this bacterium suggest that growth on methanol might have significant differences from growth on methane. In this study, both a targeted metabolomics approach and a ¹³ C tracer approach were taken to understand core carbon metabolism in M. buryatense 5GB1 during growth on methanol and to determine whether such differences occur. Our results suggest a systematic shift ofmore »« less
DOI: 10.1128/mBio.00406-19
Assessing the Efficacy of the Aerobic Methanotrophic Biofilter in Methane Hydrate Environments

Technical Report Valentine, David

In October 2008 the University of California at Santa Barbara (UCSB) initiated investigations of water column methane oxidation in methane hydrate environments, through a project funded by the National Energy Technology Laboratory (NETL) entitled: assessing the efficacy of the aerobic methanotrophic biofilter in methane hydrate environments. This Final Report describes the scientific advances and discoveries made under this award as well as the importance of these discoveries in the broader context of the research area. Benthic microbial mats inhabit the sea floor in areas where reduced chemicals such as sulfide reach the more oxidizing water that overlies the sediment. Wemore »« less
DOI: 10.2172/1083752

Full Text Available
Electroporation-Based Genetic Manipulation in Type I Methanotrophs

Journal Article Yan, Xin ; Chu, Frances ; Puri, Aaron W. ; ... - Applied and Environmental Microbiology

Methane is becoming a major candidate for a prominent carbon feedstock in the future, and the bioconversion of methane into valuable products has drawn increasing attention. In order to facilitate the use of methanotrophic organisms as industrial strains and accelerate our ability to metabolically engineer methanotrophs, simple and rapid genetic tools are needed. Electroporation is one such enabling tool, but to date it has not been successful in a group of methanotrophs of interest for the production of chemicals and fuels, the gammaproteobacterial (type I) methanotrophs. In this study, we developed electroporation techniques with a high transformation efficiency for threemore »« less
Cited by 30
DOI: 10.1128/AEM.03724-15

Full Text Available
Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts

Journal Article Henard, Calvin A. ; Akberdin, Ilya R. ; Kalyuzhnaya, Marina G. ; ... - Green Chemistry

Here, we demonstrate bioconversion of methane to muconic acid, a dicarboxylic acid that can be upgraded to an array of platform chemicals, by three gammaproteobacterial methanotrophs. All engineered methanotrophs expressing a heterologous dihydroxyshikimate dehydratase, protocatechuate decarboxylase, and catechol dioxygenase produced muconic acid from methane, with the highest titer (12.4 mg MA per L), yield (2.8 mg MA per g CH ₄), and specific productivity (1.2 mg MA per g dcw, 48 hr) synthesized by Methylotuvimicrobium buryatense, Methylococcus capsulatus, and Methylotuvimicrobium alcaliphilium, respectively. Methylotuvimicrobium alcaliphilum genome-scale model-guided strain engineering predicted that disruption of the pyruvate dehydrogenase or shikimate dehydrogenase would significantlymore »« less
DOI: 10.1039/C9GC03722E

Full Text Available

Similar Records