Title: Unlocking the Potential of Magnetotactic Bacteria as Magnetic Hyperthermia Agents

Abstract

Abstract Magnetotactic bacteria are aquatic microorganisms that internally biomineralize chains of magnetic nanoparticles (called magnetosomes) and use them as a compass. Here it is shown that magnetotactic bacteria of the strain Magnetospirillum gryphiswaldense present high potential as magnetic hyperthermia agents for cancer treatment. Their heating efficiency or specific absorption rate is determined using both calorimetric and AC magnetometry methods at different magnetic field amplitudes and frequencies. In addition, the effect of the alignment of the bacteria in the direction of the field during the hyperthermia experiments is also investigated. The experimental results demonstrate that the biological structure of the magnetosome chain of magnetotactic bacteria is perfect to enhance the hyperthermia efficiency. Furthermore, fluorescence and electron microscopy images show that these bacteria can be internalized by human lung carcinoma cells A549, and cytotoxicity studies reveal that they do not affect the viability or growth of the cancer cells. A preliminary in vitro hyperthermia study, working on clinical conditions, reveals that cancer cell proliferation is strongly affected by the hyperthermia treatment, making these bacteria promising candidates for biomedical applications.

Authors:

Gandia, David ^[1]; Gandarias, Lucía ^[2]; Rodrigo, Irati ^[1]; Robles‐García, Joshua ^[3]; Das, Raja ^[3]; Garaio, Eneko ^[4]; García, José Ángel ^[5]; Phan, Manh‐Huong ^[3]; Srikanth, Hariharan ^[3]; Orue, Iñaki ^[6]; Alonso, Javier  ^[7];

• Search DOE PAGES for author "Alonso, Javier"
• Search DOE PAGES for ORCID "0000-0003-0045-5390"

Muela, Alicia ^[8]; Fdez‐Gubieda, M. Luisa ^[9]

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+ Show Author Affiliations

1. Basque Center for Materials Applications and Nanostructures (BCMaterials) UPV/EHU Science Park Leioa 48940 Spain
2. Departamento de Inmunología Microbiología y Parasitología Universidad del País Vasco (UPV/EHU) Leioa 48940 Spain
3. Materials Institute Department of Physics University of South Florida (USF) Tampa FL 33620 USA
4. Departamento de Física Aplicada II Universidad del País Vasco (UPV/EHU) Leioa 48940 Spain, Departamento de Ciencias Universidad Pública de Navarra (UPN) Pamplona 31006 Spain
5. Basque Center for Materials Applications and Nanostructures (BCMaterials) UPV/EHU Science Park Leioa 48940 Spain, Departamento de Física Aplicada II Universidad del País Vasco (UPV/EHU) Leioa 48940 Spain
6. SGIker Medidas Magnéticas Universidad del País Vasco (UPV/EHU) Leioa 48940 Spain
7. Departamento CITIMAC Universidad de Cantabria (UC) Santander 39005 Spain
8. Basque Center for Materials Applications and Nanostructures (BCMaterials) UPV/EHU Science Park Leioa 48940 Spain, Departamento de Inmunología Microbiología y Parasitología Universidad del País Vasco (UPV/EHU) Leioa 48940 Spain
9. Basque Center for Materials Applications and Nanostructures (BCMaterials) UPV/EHU Science Park Leioa 48940 Spain, Departamento de Electricidad y Electrónica Universidad del País Vasco (UPV/EHU) Leioa 48940 Spain

Publication Date:

Tue Aug 27 00:00:00 EDT 2019

Research Org.:

Univ. of South Florida, Tampa, FL (United States)

Sponsoring Org.:

USDOE Office of Science (SC)

OSTI Identifier:

1571681

Alternate Identifier(s):

OSTI ID: 1558839; OSTI ID: 1800495

Grant/Contract Number:  

FG02-07ER46438

Resource Type:

Published Article

Journal Name:

Small

Additional Journal Information:

Journal Name: Small Journal Volume: 15 Journal Issue: 41; Journal ID: ISSN 1613-6810

Publisher:

Wiley Blackwell (John Wiley & Sons)

Country of Publication:

Germany

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; Chemistry; Science & Technology; Materials Science; Physics