skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III‐secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus

Authors:
 [1];  [2];  [1];  [3];  [3];  [4];  [5];  [5];  [4];  [6];  [3]; ORCiD logo [1]
  1. Department of Plant Pathology and Microbiology The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot Israel
  2. Department of Plant Pathology and Microbiology The Robert H. Smith Faculty of Agriculture, Food and Environment The Hebrew University of Jerusalem Rehovot Israel, Department of Microbiology University of Seville Seville Spain
  3. The School of Molecular Cell Biology and Biotechnology The George S. Wise Faculty of Life Sciences Tel Aviv University Tel Aviv Israel
  4. Department of Plant Pathology University of Georgia Athens GA USA
  5. Department of Plant Pathology and Weed Research Agricultural Research Organization The Volcani Center Bet Dagan Israel
  6. School of Plant Sciences and Food Security The George S. Wise Faculty of Life Sciences Tel Aviv University Tel Aviv Israel
Publication Date:
Sponsoring Org.:
USDOE Office of Electricity Delivery and Energy Reliability (OE), Power Systems Engineering Research and Development (R&D) (OE-10)
OSTI Identifier:
1571542
Alternate Identifier(s):
OSTI ID: 1571543
Resource Type:
Published Article
Journal Name:
Molecular Plant Pathology
Additional Journal Information:
Journal Name: Molecular Plant Pathology; Journal ID: ISSN 1464-6722
Publisher:
Wiley-Blackwell
Country of Publication:
Country unknown/Code not available
Language:
English

Citation Formats

Jiménez-Guerrero, Irene, Pérez‐Montaño, Francisco, Da Silva, Gustavo Mateus, Wagner, Naama, Shkedy, Dafna, Zhao, Mei, Pizarro, Lorena, Bar, Maya, Walcott, Ron, Sessa, Guido, Pupko, Tal, and Burdman, Saul. Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III‐secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus. Country unknown/Code not available: N. p., 2019. Web. doi:10.1111/mpp.12877.
Jiménez-Guerrero, Irene, Pérez‐Montaño, Francisco, Da Silva, Gustavo Mateus, Wagner, Naama, Shkedy, Dafna, Zhao, Mei, Pizarro, Lorena, Bar, Maya, Walcott, Ron, Sessa, Guido, Pupko, Tal, & Burdman, Saul. Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III‐secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus. Country unknown/Code not available. doi:10.1111/mpp.12877.
Jiménez-Guerrero, Irene, Pérez‐Montaño, Francisco, Da Silva, Gustavo Mateus, Wagner, Naama, Shkedy, Dafna, Zhao, Mei, Pizarro, Lorena, Bar, Maya, Walcott, Ron, Sessa, Guido, Pupko, Tal, and Burdman, Saul. Wed . "Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III‐secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus". Country unknown/Code not available. doi:10.1111/mpp.12877.
@article{osti_1571542,
title = {Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III‐secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus},
author = {Jiménez-Guerrero, Irene and Pérez‐Montaño, Francisco and Da Silva, Gustavo Mateus and Wagner, Naama and Shkedy, Dafna and Zhao, Mei and Pizarro, Lorena and Bar, Maya and Walcott, Ron and Sessa, Guido and Pupko, Tal and Burdman, Saul},
abstractNote = {},
doi = {10.1111/mpp.12877},
journal = {Molecular Plant Pathology},
number = ,
volume = ,
place = {Country unknown/Code not available},
year = {2019},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1111/mpp.12877

Save / Share:

Works referenced in this record:

Opening the Ralstonia solanacearum type III effector tool box: insights into host cell subversion mechanisms
journal, August 2014


Nicotiana benthamiana : Its History and Future as a Model for Plant–Pathogen Interactions
journal, August 2008

  • Goodin, Michael M.; Zaitlin, David; Naidu, Rayapati A.
  • Molecular Plant-Microbe Interactions, Vol. 21, Issue 8
  • DOI: 10.1094/MPMI-21-8-1015

Mechanisms of ROS Regulation of Plant Development and Stress Responses
journal, June 2019


Analyses of the secretomes of Erwinia amylovora and selected hrp mutants reveal novel type III secreted proteins and an effect of HrpJ on extracellular harpin levels
journal, January 2007


Cutadapt removes adapter sequences from high-throughput sequencing reads
journal, May 2011


MEME-ChIP: motif analysis of large DNA datasets
journal, April 2011


STAR: ultrafast universal RNA-seq aligner
journal, October 2012


Photoactivation of GFP reveals protein dynamics within the endoplasmic reticulum membrane
journal, October 2005

  • Runions, John; Brach, Thorsten; Kühner, Sebastian
  • Journal of Experimental Botany, Vol. 57, Issue 1
  • DOI: 10.1093/jxb/eri289

HrpG regulates type II secretory proteins in Xanthomonas axonopodis pv. citri
journal, February 2008

  • Yamazaki, Akihiro; Hirata, Hisae; Tsuyumu, Shinji
  • Journal of General Plant Pathology, Vol. 74, Issue 2
  • DOI: 10.1007/s10327-008-0075-7

prhJ and hrpG, two new components of the plant signal-dependent regulatory cascade controlled by PrhA in Ralstonia solanacearum
journal, January 1999


Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes
journal, December 1995


The vapB–vapC Operon of Acidovorax citrulli Functions as a Bona-fide Toxin–Antitoxin Module
journal, January 2016


Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
journal, May 2010

  • Trapnell, Cole; Williams, Brian A.; Pertea, Geo
  • Nature Biotechnology, Vol. 28, Issue 5
  • DOI: 10.1038/nbt.1621

Behind the lines–actions of bacterial type III effector proteins in plant cells
journal, August 2016


Combined prediction of Tat and Sec signal peptides with hidden Markov models
journal, September 2010


Differential expression analysis for sequence count data
journal, October 2010


Integrated Regulation of the Type III Secretion System and Other Virulence Determinants in Ralstonia solanacearum
journal, January 2006


Repertoire, unified nomenclature and evolution of the Type III effector gene set in the Ralstonia solanacearum species complex
journal, January 2013


SignalP 4.0: discriminating signal peptides from transmembrane regions
journal, September 2011

  • Petersen, Thomas Nordahl; Brunak, Søren; von Heijne, Gunnar
  • Nature Methods, Vol. 8, Issue 10
  • DOI: 10.1038/nmeth.1701

Molecular, Physiological, and Host-Range Characterization of Acidovorax avenae subsp. citrulli Isolates from Watermelon and Melon in Israel
journal, December 2005

  • Burdman, Saul; Kots, Nadia; Kritzman, Giora
  • Plant Disease, Vol. 89, Issue 12
  • DOI: 10.1094/PD-89-1339

Plant–bacterial pathogen interactions mediated by type III effectors
journal, August 2012


Specific Binding of the Xanthomonas campestris pv. vesicatoria AraC-Type Transcriptional Activator HrpX to Plant-Inducible Promoter Boxes
journal, August 2006

  • Koebnik, R.; Kruger, A.; Thieme, F.
  • Journal of Bacteriology, Vol. 188, Issue 21
  • DOI: 10.1128/JB.00795-06

Evolution of Plant Pathogenesis in Pseudomonas syringae : A Genomics Perspective
journal, September 2011


Direct biochemical evidence for type III secretion-dependent translocation of the AvrBs2 effector protein into plant cells
journal, June 2002

  • Casper-Lindley, C.; Dahlbeck, D.; Clark, E. T.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 12
  • DOI: 10.1073/pnas.122220299

Allelic Variants of the Pseudomonas syringae Type III Effector HopZ1 Are Differentially Recognized by Plant Resistance Systems
journal, February 2009

  • Zhou, Huanbin; Morgan, Robyn L.; Guttman, David S.
  • Molecular Plant-Microbe Interactions, Vol. 22, Issue 2
  • DOI: 10.1094/MPMI-22-2-0176

Advantages of combined transmembrane topology and signal peptide prediction--the Phobius web server
journal, May 2007

  • Kall, L.; Krogh, A.; Sonnhammer, E. L. L.
  • Nucleic Acids Research, Vol. 35, Issue Web Server
  • DOI: 10.1093/nar/gkm256

Pathogen perception by NLRs in plants and animals: Parallel worlds
journal, June 2016


Bacterial Type III Secretion Systems: Specialized Nanomachines for Protein Delivery into Target Cells
journal, September 2014


Deletions in the Repertoire of Pseudomonas syringae pv. tomato DC3000 Type III Secretion Effector Genes Reveal Functional Overlap among Effectors
journal, April 2009


Evidence for HrpXo-Dependent Expression of Type II Secretory Proteins in Xanthomonas oryzae pv. oryzae
journal, February 2004


Widespread distribution and fitness contribution of Xanthomonas campestris avirulence gene avrBs2
journal, July 1990

  • Kearney, Brian; Staskawicz, Brian J.
  • Nature, Vol. 346, Issue 6282
  • DOI: 10.1038/346385a0

Phytopathogen type III effector weaponry and their plant targets
journal, August 2008

  • Block, Anna; Li, Guangyong; Fu, Zheng Qing
  • Current Opinion in Plant Biology, Vol. 11, Issue 4
  • DOI: 10.1016/j.pbi.2008.06.007

Arginine/Lysine Residues in the Cytoplasmic Tail Promote ER Export of Plant Glycosylation Enzymes
journal, October 2008


Differences in Pathogenicity between two Genetically Distinct Groups of Acidovorax avenae subsp. citrulli on Cucurbit Hosts
journal, May 2004


Current Status of the Gene-For-Gene Concept
journal, September 1971


A new mathematical model for relative quantification in real-time RT-PCR
journal, May 2001


The plant immune system
journal, November 2006

  • Jones, Jonathan D. G.; Dangl, Jeffery L.
  • Nature, Vol. 444, Issue 7117
  • DOI: 10.1038/nature05286

Genome-Scale Identification of Legionella pneumophila Effectors Using a Machine Learning Approach
journal, July 2009


A high-throughput, near-saturating screen for type III effector genes from Pseudomonas syringae
journal, February 2005

  • Chang, J. H.; Urbach, J. M.; Law, T. F.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 7
  • DOI: 10.1073/pnas.0409660102

Comparative Analysis of Type III Secreted Effector Genes Reflects Divergence of Acidovorax citrulli Strains into Three Distinct Lineages
journal, November 2014


Characterization of the Xanthomonas AvrXv4 Effector, a SUMO Protease Translocated into Plant Cells
journal, June 2004

  • Roden, Julie; Eardley, Leah; Hotson, Andrew
  • Molecular Plant-Microbe Interactions, Vol. 17, Issue 6
  • DOI: 10.1094/MPMI.2004.17.6.633

HrpG and HrpX Play Global Roles in Coordinating Different Virulence Traits of Xanthomonas axonopodis pv. citri
journal, June 2011

  • Guo, Yinping; Figueiredo, Francisco; Jones, Jeffery
  • Molecular Plant-Microbe Interactions, Vol. 24, Issue 6
  • DOI: 10.1094/MPMI-09-10-0209

Simple and rapid fluorimetric method for DNA microassay
journal, November 1977


HrpG, a Key hrp Regulatory Protein of Xanthomonas campestris pv. vesicatoria Is Homologous to Two-Component Response Regulators
journal, January 1996

  • Wengelnik, Kai
  • Molecular Plant-Microbe Interactions, Vol. 9, Issue 8
  • DOI: 10.1094/MPMI-9-0704

Gene-For-Gene Relationships Specifying Disease Resistance in Xanthomonas campestris pv. vesicatoria - Pepper Interactions
journal, January 1990

  • Minsavage, G. V.
  • Molecular Plant-Microbe Interactions, Vol. 3, Issue 1
  • DOI: 10.1094/MPMI-3-041

A genetic screen to isolate type III effectors translocated into pepper cells during Xanthomonas infection
journal, November 2004

  • Roden, J. A.; Belt, B.; Ross, J. B.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 47
  • DOI: 10.1073/pnas.0407383101

Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato
journal, November 1999

  • Tai, T. H.; Dahlbeck, D.; Clark, E. T.
  • Proceedings of the National Academy of Sciences, Vol. 96, Issue 24
  • DOI: 10.1073/pnas.96.24.14153

Type IV Pili Are Required for Virulence, Twitching Motility, and Biofilm Formation of Acidovorax avenae subsp. citrulli
journal, August 2009

  • Bahar, Ofir; Goffer, Tal; Burdman, Saul
  • Molecular Plant-Microbe Interactions, Vol. 22, Issue 8
  • DOI: 10.1094/MPMI-22-8-0909

An improved suicide vector for construction of chromosomal insertion mutations in bacteria
journal, September 1992


Insights from the Genome Sequence of Acidovorax citrulli M6, a Group I Strain of the Causal Agent of Bacterial Fruit Blotch of Cucurbits
journal, April 2016

  • Eckshtain-Levi, Noam; Shkedy, Dafna; Gershovits, Michael
  • Frontiers in Microbiology, Vol. 7
  • DOI: 10.3389/fmicb.2016.00430

Multiple Approaches to a Complete Inventory of Pseudomonas syringae pv. tomato DC3000 Type III Secretion System Effector Proteins
journal, November 2006

  • Schechter, Lisa M.; Vencato, Monica; Jordan, Katy L.
  • Molecular Plant-Microbe Interactions, Vol. 19, Issue 11
  • DOI: 10.1094/MPMI-19-1180

Complete Assembly of the Genome of an Acidovorax citrulli Strain Reveals a Naturally Occurring Plasmid in This Species
journal, June 2019

  • Yang, Rongzhi; Santos Garcia, Diego; Pérez Montaño, Francisco
  • Frontiers in Microbiology, Vol. 10
  • DOI: 10.3389/fmicb.2019.01400

Wound signalling in plants
journal, January 2001

  • León, José; Rojo, Enrique; Sánchez‐Serrano, José J.
  • Journal of Experimental Botany, Vol. 52, Issue 354
  • DOI: 10.1093/jxb/52.354.1

Fiji: an open-source platform for biological-image analysis
journal, June 2012

  • Schindelin, Johannes; Arganda-Carreras, Ignacio; Frise, Erwin
  • Nature Methods, Vol. 9, Issue 7
  • DOI: 10.1038/nmeth.2019

Pathogenomics of the Ralstonia solanacearum Species Complex
journal, September 2012


Bacterial fruit blotch: A threat to the cucurbit industry
journal, September 2010

  • Bahar, Ofir; Burdman, Saul
  • Israel Journal of Plant Sciences, Vol. 58, Issue 1
  • DOI: 10.1560/IJPS.58.1.19

Genome-Wide Analysis of Gene Expression in Ralstonia solanacearum Reveals That the hrpB Gene Acts as a Regulatory Switch Controlling Multiple Virulence Pathways
journal, September 2005

  • Occhialini, Alessandra; Cunnac, Sébastien; Reymond, Nancie
  • Molecular Plant-Microbe Interactions, Vol. 18, Issue 9
  • DOI: 10.1094/MPMI-18-0938

The type III effectors of Xanthomonas
journal, November 2009


Gateway-compatible vectors for plant functional genomics and proteomics
journal, February 2006


Targeting of plant pattern recognition receptor-triggered immunity by bacterial type-III secretion system effectors
journal, February 2015


Identification of Novel Coxiella burnetii Icm/Dot Effectors and Genetic Analysis of Their Involvement in Modulating a Mitogen-Activated Protein Kinase Pathway
journal, June 2014

  • Lifshitz, Ziv; Burstein, David; Schwartz, Kierstyn
  • Infection and Immunity, Vol. 82, Issue 9
  • DOI: 10.1128/IAI.01729-14

Revealing the inventory of type III effectors in Pantoea agglomerans gall-forming pathovars using draft genome sequences and a machine-learning approach : Inventory of
journal, February 2017

  • Nissan, Gal; Gershovits, Michael; Morozov, Michael
  • Molecular Plant Pathology, Vol. 19, Issue 2
  • DOI: 10.1111/mpp.12528

Investigating Intraspecific Variation of Acidovorax avenae subsp. citrulli Using DNA Fingerprinting and Whole Cell Fatty Acid Analysis
journal, February 2000


The Sequence Alignment/Map format and SAMtools
journal, June 2009


Random Forests
journal, January 2001


Unified nomenclature for broadly conserved hrp genes of phytopathogenic bacteria
journal, May 1996


Regulation and secretion of Xanthomonas virulence factors
journal, March 2010


Identification of novel X anthomonas euvesicatoria type III effector proteins by a machine-learning approach : Novel
journal, August 2015

  • Teper, Doron; Burstein, David; Salomon, Dor
  • Molecular Plant Pathology, Vol. 17, Issue 3
  • DOI: 10.1111/mpp.12288