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Title: New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Journal Article · · Biomedical Optics Express

Properties of a novel ultra-fast optical imager, Tpx3Cam, were explored for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. Here, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O2 sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O2 in various chemical and biological samples.

Research Organization:
Brookhaven National Laboratory (BNL), Upton, NY (United States)
Sponsoring Organization:
USDOE Office of Science (SC), High Energy Physics (HEP) (SC-25); Science Foundation Ireland
Grant/Contract Number:
SC0012704
OSTI ID:
1571397
Report Number(s):
BNL--212226-2019-JAAM
Journal Information:
Biomedical Optics Express, Journal Name: Biomedical Optics Express Journal Issue: 1 Vol. 11; ISSN 2156-7085
Publisher:
Optical Society of AmericaCopyright Statement
Country of Publication:
United States
Language:
English

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