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Title: New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Abstract

Properties of a novel ultra-fast optical imager, Tpx3Cam, were explored for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. Here, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O2 sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O2 in various chemical and biological samples.

Authors:
; ; ; ; ORCiD logo; ; ORCiD logo
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), High Energy Physics (HEP); Science Foundation Ireland
OSTI Identifier:
1571397
Report Number(s):
BNL-212226-2019-JAAM
Journal ID: ISSN 2156-7085
Grant/Contract Number:  
SC0012704
Resource Type:
Accepted Manuscript
Journal Name:
Biomedical Optics Express
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 2156-7085
Publisher:
Optical Society of America
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; Phosphorescence Lifetime IMaging (PLIM); Fluorescence Lifetime IMaging (FLIM); Time Correlated Single Photon Counting (TCSPC); Macroscopic imaging; Oxygen sensing and imaging; Tpx3Cam

Citation Formats

Sen, Rajannya, Hirvonen, Liisa M., Zhdanov, Alexander, Svihra, Peter, Andersson-Engels, Stefan, Nomerotski, Andrei, and Papkovsky, Dmitri. New luminescence lifetime macro-imager based on a Tpx3Cam optical camera. United States: N. p., 2019. Web. doi:10.1364/BOE.11.000077.
Sen, Rajannya, Hirvonen, Liisa M., Zhdanov, Alexander, Svihra, Peter, Andersson-Engels, Stefan, Nomerotski, Andrei, & Papkovsky, Dmitri. New luminescence lifetime macro-imager based on a Tpx3Cam optical camera. United States. doi:https://doi.org/10.1364/BOE.11.000077
Sen, Rajannya, Hirvonen, Liisa M., Zhdanov, Alexander, Svihra, Peter, Andersson-Engels, Stefan, Nomerotski, Andrei, and Papkovsky, Dmitri. Thu . "New luminescence lifetime macro-imager based on a Tpx3Cam optical camera". United States. doi:https://doi.org/10.1364/BOE.11.000077. https://www.osti.gov/servlets/purl/1571397.
@article{osti_1571397,
title = {New luminescence lifetime macro-imager based on a Tpx3Cam optical camera},
author = {Sen, Rajannya and Hirvonen, Liisa M. and Zhdanov, Alexander and Svihra, Peter and Andersson-Engels, Stefan and Nomerotski, Andrei and Papkovsky, Dmitri},
abstractNote = {Properties of a novel ultra-fast optical imager, Tpx3Cam, were explored for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. Here, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O2 sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O2 in various chemical and biological samples.},
doi = {10.1364/BOE.11.000077},
journal = {Biomedical Optics Express},
number = 1,
volume = 11,
place = {United States},
year = {2019},
month = {12}
}

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