New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Sen, Rajannya; Hirvonen, Liisa M.; Zhdanov, Alexander; Svihra, Peter; Andersson-Engels, Stefan; Nomerotski, Andrei; Papkovsky, Dmitri

doi:10.1364/BOE.11.000077

Title: New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Abstract

Properties of a novel ultra-fast optical imager, Tpx3Cam, were explored for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. Here, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O₂ sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O₂ in various chemical and biological samples.

Authors:

Sen, Rajannya; Hirvonen, Liisa M.; Zhdanov, Alexander; Svihra, Peter;

; Nomerotski, Andrei;

Publication Date:: 2019-12-05

Research Org.:: Brookhaven National Lab. (BNL), Upton, NY (United States)

Sponsoring Org.:: USDOE Office of Science (SC), High Energy Physics (HEP); Science Foundation Ireland

OSTI Identifier:: 1571397

Report Number(s):: BNL-212226-2019-JAAM
Journal ID: ISSN 2156-7085

Grant/Contract Number:: SC0012704

Resource Type:: Accepted Manuscript

Journal Name:: Biomedical Optics Express

Additional Journal Information:: Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 2156-7085

Publisher:: Optical Society of America

Country of Publication:: United States

Language:: English

Subject:: 72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS; Phosphorescence Lifetime IMaging (PLIM); Fluorescence Lifetime IMaging (FLIM); Time Correlated Single Photon Counting (TCSPC); Macroscopic imaging; Oxygen sensing and imaging; Tpx3Cam

Citation Formats


                    Sen, Rajannya, Hirvonen, Liisa M., Zhdanov, Alexander, Svihra, Peter, Andersson-Engels, Stefan, Nomerotski, Andrei, and Papkovsky, Dmitri. New luminescence lifetime macro-imager based on a Tpx3Cam optical camera.  United States: N. p., 2019. 
Web.  doi:10.1364/BOE.11.000077.

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                    Sen, Rajannya, Hirvonen, Liisa M., Zhdanov, Alexander, Svihra, Peter, Andersson-Engels, Stefan, Nomerotski, Andrei, & Papkovsky, Dmitri. New luminescence lifetime macro-imager based on a Tpx3Cam optical camera.  United States.  https://doi.org/10.1364/BOE.11.000077

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                    Sen, Rajannya, Hirvonen, Liisa M., Zhdanov, Alexander, Svihra, Peter, Andersson-Engels, Stefan, Nomerotski, Andrei, and Papkovsky, Dmitri. Thu .  
"New luminescence lifetime macro-imager based on a Tpx3Cam optical camera".  United States.  https://doi.org/10.1364/BOE.11.000077.  https://www.osti.gov/servlets/purl/1571397.

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@article{osti_1571397,

  title        = {New luminescence lifetime macro-imager based on a Tpx3Cam optical camera},

  author       = {Sen, Rajannya and Hirvonen, Liisa M. and Zhdanov, Alexander and Svihra, Peter and Andersson-Engels, Stefan and Nomerotski, Andrei and Papkovsky, Dmitri},

  abstractNote = {Properties of a novel ultra-fast optical imager, Tpx3Cam, were explored for macroscopic wide-field phosphorescent lifetime imaging (PLIM) applications. The camera is based on a novel optical sensor and Timepix3 readout chip with time resolution of 1.6 ns, recording of photon arrival time and time over threshold for each pixel, and readout rate of 80 megapixels per second. Here, we coupled the camera to an image intensifier, a 760 nm emission filter and a 50 mm lens, and with a super-bright 625nm LED providing pulsed excitation of a 18x18mm sample area. The resulting macro-imager with compact and rigid optical alignment of its main components was characterised using planar phosphorescent O2 sensors and a resolution plate mask. Several acquisition and image processing algorithms were evaluated to optimise the system resolution and performance for the wide-field PLIM, followed by imaging a variety of phosphorescent samples. The new PLIM system looks promising, particularly for phosphorescence lifetime-based imaging of O2 in various chemical and biological samples.},

  doi          = {10.1364/BOE.11.000077},

  journal      = {Biomedical Optics Express},

  number       = 1,

  volume       = 11,

  place        = {United States},

  year         = {2019},

  month        = {12}

}

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Title: New luminescence lifetime macro-imager based on a Tpx3Cam optical camera

Abstract

Citation Formats

Fluorescence Lifetime Techniques in Medical Applications journal, January 2012

Applications of fluorescence lifetime imaging in clinical medicine journal, November 2017

Fluorescence Lifetime Imaging Microscopy of Intracellular Glucose Dynamics journal, November 2012

Fast fluorescence lifetime imaging of calcium in living cells journal, January 2004

Fluorescence lifetime imaging of calcium using Quin-2 journal, March 1992

Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging journal, February 2018

Fluorescence Lifetime Imaging Microscopy for the Detection of Intracellular pH with Quantum Dot Nanosensors journal, June 2013

Fluorescence lifetime-resolved pH imaging of living cells journal, March 2003

Optical probes and techniques for O2 measurement in live cells and tissue journal, January 2012

Luminescent barometry in wind tunnels journal, November 1990

Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin journal, January 2012

Fluorescence lifetime imaging ophthalmoscopy journal, September 2017

Cellular Metabolic Heterogeneity In Vivo Is Recapitulated in Tumor Organoids journal, June 2019

Recent Advances in Fluorescence Lifetime Analytical Microsystems: Contact Optics and CMOS Time-Resolved Electronics journal, December 2017

Wide-field TCSPC: methods and applications journal, December 2016

Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging journal, January 2017

Wide-field time-correlated single-photon counting (TCSPC) lifetime microscopy with microsecond time resolution journal, January 2014

Photon arrival timing with sub-camera exposure time resolution in wide-field time-resolved photon counting imaging journal, January 2010

Sub- μ s time resolution in wide-field time-correlated single photon counting microscopy obtained from the photon event phosphor decay journal, February 2015

TimepixCam: a fast optical imager with time-stamping journal, March 2016

Imaging and time stamping of photons with nanosecond resolution in Timepix based optical cameras journal, September 2019

Erratum to “Timepix, a 65 k programmable pixel readout chip for arrival time, energy and/or photon counting measurements” [Nucl. Instr. and Meth. A. 581 (2007) 485–494] journal, January 2008

Characterization of TimepixCam, a fast imager for the time-stamping of optical photons journal, January 2017

Photon counting phosphorescence lifetime imaging with TimepixCam journal, January 2017

SPIDR: a read-out system for Medipix3 & Timepix3 journal, December 2015

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X-ray and gamma imaging with Medipix and Timepix detectors in medical research journal, August 2019

Time-resolved ion imaging at free-electron lasers using TimepixCam journal, February 2018

In vivo imaging system for explants analysis—A new approach for assessment of cell transplantation effects in large animal models journal, September 2017

Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein–protein interactions using global analysis journal, August 2008

Planar implantable sensor for in vivo measurement of cellular oxygen metabolism in brain tissue journal, April 2017

Highly Photostable Near-Infrared Fluorescent pH Indicators and Sensors Based on BF 2 -Chelated Tetraarylazadipyrromethene Dyes journal, July 2012

In vivo imaging of brain metabolism activity using a phosphorescent oxygen-sensitive probe journal, June 2013

Coincidence velocity map imaging using Tpx3Cam, a time stamping optical camera with 1.5 ns timing resolution journal, November 2017

Timewalk correction for the Timepix3 chip obtained with real particle data journal, June 2019

Discrete O2 sensors produced by a spotting method on polyolefin fabric substrates journal, November 2014

Monitoring of cell oxygenation and responses to metabolic stimulation by intracellular oxygen sensing technique journal, July 2010

Analysis of decay kinetics of the cytosolic calcium transient induced by oxytocin in rat myometrium smooth muscle cells journal, February 2021

Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM journal, March 2020

Fluorescence lifetime imaging ophthalmoscopy journal, September 2017

FLUORESCENCE LIFETIME IMAGING OPHTHALMOSCOPY: Findings After Surgical Reattachment of Macula-Off Rhegmatogenous Retinal Detachment journal, December 2019

Coincidence velocity map imaging using Tpx3Cam, a time stamping optical camera with 1.5 ns timing resolution text, January 2017

Timewalk correction for the Timepix3 chip obtained with real particle data text, January 2019

Fluorescence Lifetime Techniques in Medical Applications
journal, January 2012

Applications of fluorescence lifetime imaging in clinical medicine
journal, November 2017

Fluorescence Lifetime Imaging Microscopy of Intracellular Glucose Dynamics
journal, November 2012

Fast fluorescence lifetime imaging of calcium in living cells
journal, January 2004

Fluorescence lifetime imaging of calcium using Quin-2
journal, March 1992

Monitoring intracellular nanomolar calcium using fluorescence lifetime imaging
journal, February 2018

Fluorescence Lifetime Imaging Microscopy for the Detection of Intracellular pH with Quantum Dot Nanosensors
journal, June 2013

Fluorescence lifetime-resolved pH imaging of living cells
journal, March 2003

Optical probes and techniques for O2 measurement in live cells and tissue
journal, January 2012

Luminescent barometry in wind tunnels
journal, November 1990

Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin
journal, January 2012

Fluorescence lifetime imaging ophthalmoscopy
journal, September 2017

Cellular Metabolic Heterogeneity In Vivo Is Recapitulated in Tumor Organoids
journal, June 2019

Recent Advances in Fluorescence Lifetime Analytical Microsystems: Contact Optics and CMOS Time-Resolved Electronics
journal, December 2017

Wide-field TCSPC: methods and applications
journal, December 2016

Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging
journal, January 2017

Wide-field time-correlated single-photon counting (TCSPC) lifetime microscopy with microsecond time resolution
journal, January 2014

Photon arrival timing with sub-camera exposure time resolution in wide-field time-resolved photon counting imaging
journal, January 2010

Sub- μ s time resolution in wide-field time-correlated single photon counting microscopy obtained from the photon event phosphor decay
journal, February 2015

TimepixCam: a fast optical imager with time-stamping
journal, March 2016

Imaging and time stamping of photons with nanosecond resolution in Timepix based optical cameras
journal, September 2019

Erratum to “Timepix, a 65 k programmable pixel readout chip for arrival time, energy and/or photon counting measurements” [Nucl. Instr. and Meth. A. 581 (2007) 485–494]
journal, January 2008

Characterization of TimepixCam, a fast imager for the time-stamping of optical photons
journal, January 2017

Photon counting phosphorescence lifetime imaging with TimepixCam
journal, January 2017

SPIDR: a read-out system for Medipix3 & Timepix3
journal, December 2015

Medipix2/Timepix detector for time resolved Transmission Electron Microscopy
journal, December 2011

X-ray and gamma imaging with Medipix and Timepix detectors in medical research
journal, August 2019

Time-resolved ion imaging at free-electron lasers using TimepixCam
journal, February 2018

In vivo imaging system for explants analysis—A new approach for assessment of cell transplantation effects in large animal models
journal, September 2017

Multiphoton time-domain fluorescence lifetime imaging microscopy: practical application to protein–protein interactions using global analysis
journal, August 2008

Planar implantable sensor for in vivo measurement of cellular oxygen metabolism in brain tissue
journal, April 2017

Highly Photostable Near-Infrared Fluorescent pH Indicators and Sensors Based on BF ₂ -Chelated Tetraarylazadipyrromethene Dyes
journal, July 2012

In vivo imaging of brain metabolism activity using a phosphorescent oxygen-sensitive probe
journal, June 2013

Coincidence velocity map imaging using Tpx3Cam, a time stamping optical camera with 1.5 ns timing resolution
journal, November 2017

Timewalk correction for the Timepix3 chip obtained with real particle data
journal, June 2019

Discrete O2 sensors produced by a spotting method on polyolefin fabric substrates
journal, November 2014

Monitoring of cell oxygenation and responses to metabolic stimulation by intracellular oxygen sensing technique
journal, July 2010

Analysis of decay kinetics of the cytosolic calcium transient induced by oxytocin in rat myometrium smooth muscle cells
journal, February 2021

Quantitative real-time imaging of intracellular FRET biosensor dynamics using rapid multi-beam confocal FLIM
journal, March 2020

Fluorescence lifetime imaging ophthalmoscopy
journal, September 2017

FLUORESCENCE LIFETIME IMAGING OPHTHALMOSCOPY: Findings After Surgical Reattachment of Macula-Off Rhegmatogenous Retinal Detachment
journal, December 2019

Coincidence velocity map imaging using Tpx3Cam, a time stamping optical camera with 1.5 ns timing resolution
text, January 2017

Timewalk correction for the Timepix3 chip obtained with real particle data
text, January 2019