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Title: Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities

Abstract

Phototrophic communities of photosynthetic algae or cyanobacteria and heterotrophic bacteria or fungi are pervasive throughout the environment. How interactions between members contribute to the resilience and affect the fitness of phototrophic communities is not fully understood. In this work, we integrated metatranscriptomics, metabolomics and phenotyping with computational modelling to reveal condition-dependent secretion and cross-feeding of metabolites in a synthetic community. We discovered that interactions between members are highly dynamic and are driven by the availability of organic and inorganic nutrients. Environmental factors, such as ammonia concentration, influenced community stability by shifting members from collaborating to competing. Moreover, overall fitness was dependent on genotype and streamlined genomes improved growth of the entire community. Our mechanistic framework offers insights into the physiology and metabolic response to environmental and genetic perturbation of these ubiquitous microbial associations.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2];  [1];  [2];  [2];  [1];  [3];  [2]; ORCiD logo [1]
  1. Univ. of California, San Diego, CA (United States)
  2. Johns Hopkins Univ., Baltimore, MD (United States)
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1571390
Report Number(s):
NREL/JA-5100-74455
Journal ID: ISSN 2058-5276
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Nature Microbiology
Additional Journal Information:
Journal Volume: 4; Journal Issue: 12; Journal ID: ISSN 2058-5276
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; bioenergy; industrial biotechnology

Citation Formats

Zuñiga, Cristal, Li, Chien-Ting, Yu, Geng, Al-Bassam, Mahmoud M., Li, Tingting, Jiang, Liqun, Zaramela, Livia S., Guarnieri, Michael, Betenbaugh, Michael J., and Zengler, Karsten. Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities. United States: N. p., 2019. Web. doi:10.1038/s41564-019-0567-6.
Zuñiga, Cristal, Li, Chien-Ting, Yu, Geng, Al-Bassam, Mahmoud M., Li, Tingting, Jiang, Liqun, Zaramela, Livia S., Guarnieri, Michael, Betenbaugh, Michael J., & Zengler, Karsten. Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities. United States. doi:10.1038/s41564-019-0567-6.
Zuñiga, Cristal, Li, Chien-Ting, Yu, Geng, Al-Bassam, Mahmoud M., Li, Tingting, Jiang, Liqun, Zaramela, Livia S., Guarnieri, Michael, Betenbaugh, Michael J., and Zengler, Karsten. Mon . "Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities". United States. doi:10.1038/s41564-019-0567-6.
@article{osti_1571390,
title = {Environmental stimuli drive a transition from cooperation to competition in synthetic phototrophic communities},
author = {Zuñiga, Cristal and Li, Chien-Ting and Yu, Geng and Al-Bassam, Mahmoud M. and Li, Tingting and Jiang, Liqun and Zaramela, Livia S. and Guarnieri, Michael and Betenbaugh, Michael J. and Zengler, Karsten},
abstractNote = {Phototrophic communities of photosynthetic algae or cyanobacteria and heterotrophic bacteria or fungi are pervasive throughout the environment. How interactions between members contribute to the resilience and affect the fitness of phototrophic communities is not fully understood. In this work, we integrated metatranscriptomics, metabolomics and phenotyping with computational modelling to reveal condition-dependent secretion and cross-feeding of metabolites in a synthetic community. We discovered that interactions between members are highly dynamic and are driven by the availability of organic and inorganic nutrients. Environmental factors, such as ammonia concentration, influenced community stability by shifting members from collaborating to competing. Moreover, overall fitness was dependent on genotype and streamlined genomes improved growth of the entire community. Our mechanistic framework offers insights into the physiology and metabolic response to environmental and genetic perturbation of these ubiquitous microbial associations.},
doi = {10.1038/s41564-019-0567-6},
journal = {Nature Microbiology},
number = 12,
volume = 4,
place = {United States},
year = {2019},
month = {10}
}

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