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Title: The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils

Abstract

After a forest wildfire, the microbial communities have a transient alteration in their composition. The role of the soil microbial community in the recovery of an ecosystem following such an event remains poorly understood. Thus, it is necessary to understand the plant-microbe interactions that occur in burned soils. By high-throughput sequencing, we identified the main bacterial taxa of burnt holm-oak rhizosphere, then we obtained an isolate collection of the most abundant genus and its growth promoting activities were characterised. 16S rRNA amplicon sequencing showed that the genus Arthrobacter comprised more than 21% of the total community. 55 Arthrobacter strains were isolated and characterized using RAPDs and sequencing of the almost complete 16S rRNA gene. Our results indicate that isolated Arthrobacter strains present a very high genetic diversity, and they could play an important ecological role in interaction with the host plant by enhancing aerial growth. Most of the selected strains exhibited a great ability to degrade organic polymers in vitro as well as possibly presenting a direct mechanism for plant growth promotion. Finally, all the above data suggests that Arthrobacter can be considered as an excellent PGP rhizobacterium that may play an important role in the recovery of burned holm-oakmore » forests.« less

Authors:
 [1];  [2];  [1];  [1];  [2];  [1];  [3]; ORCiD logo [1]
  1. Consejo Superior de Investigaciones Científicas (CSIC), Granada (Spain). Estación Experimental del Zaidín, Dept. de Microbiología del Suelo y Sistemas Simbióticos
  2. Univ. de Salamanca (Spain). Dept. de Microbiología y Genética
  3. DOE Joint Genome Inst., Walnut Creek, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Biological Systems Science Division
OSTI Identifier:
1571090
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 7; Journal Issue: 1; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Fernández-González, Antonio J., Martínez-Hidalgo, Pilar, Cobo-Díaz, José F., Villadas, Pablo J., Martínez-Molina, Eustoquio, Toro, Nicolás, Tringe, Susannah G., and Fernández-López, Manuel. The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils. United States: N. p., 2017. Web. doi:10.1038/s41598-017-06112-3.
Fernández-González, Antonio J., Martínez-Hidalgo, Pilar, Cobo-Díaz, José F., Villadas, Pablo J., Martínez-Molina, Eustoquio, Toro, Nicolás, Tringe, Susannah G., & Fernández-López, Manuel. The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils. United States. doi:10.1038/s41598-017-06112-3.
Fernández-González, Antonio J., Martínez-Hidalgo, Pilar, Cobo-Díaz, José F., Villadas, Pablo J., Martínez-Molina, Eustoquio, Toro, Nicolás, Tringe, Susannah G., and Fernández-López, Manuel. Thu . "The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils". United States. doi:10.1038/s41598-017-06112-3. https://www.osti.gov/servlets/purl/1571090.
@article{osti_1571090,
title = {The rhizosphere microbiome of burned holm-oak: potential role of the genus Arthrobacter in the recovery of burned soils},
author = {Fernández-González, Antonio J. and Martínez-Hidalgo, Pilar and Cobo-Díaz, José F. and Villadas, Pablo J. and Martínez-Molina, Eustoquio and Toro, Nicolás and Tringe, Susannah G. and Fernández-López, Manuel},
abstractNote = {After a forest wildfire, the microbial communities have a transient alteration in their composition. The role of the soil microbial community in the recovery of an ecosystem following such an event remains poorly understood. Thus, it is necessary to understand the plant-microbe interactions that occur in burned soils. By high-throughput sequencing, we identified the main bacterial taxa of burnt holm-oak rhizosphere, then we obtained an isolate collection of the most abundant genus and its growth promoting activities were characterised. 16S rRNA amplicon sequencing showed that the genus Arthrobacter comprised more than 21% of the total community. 55 Arthrobacter strains were isolated and characterized using RAPDs and sequencing of the almost complete 16S rRNA gene. Our results indicate that isolated Arthrobacter strains present a very high genetic diversity, and they could play an important ecological role in interaction with the host plant by enhancing aerial growth. Most of the selected strains exhibited a great ability to degrade organic polymers in vitro as well as possibly presenting a direct mechanism for plant growth promotion. Finally, all the above data suggests that Arthrobacter can be considered as an excellent PGP rhizobacterium that may play an important role in the recovery of burned holm-oak forests.},
doi = {10.1038/s41598-017-06112-3},
journal = {Scientific Reports},
number = 1,
volume = 7,
place = {United States},
year = {2017},
month = {7}
}

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