Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars

Padmanabhan, Sasisanker; Schwyter, Philippe; Liu, Zhongguo; Poon, Geoffrey; Bell, Alexis T.; Prausnitz, John M.

doi:10.1007/s13205-015-0344-z

Title: Delignification of miscanthus using ethylenediamine (EDA) with or without ammonia and subsequent enzymatic hydrolysis to sugars

Journal Article · 10 January 2016 · 3 Biotech

DOI: https://doi.org/10.1007/s13205-015-0344-z · OSTI ID:1571069

Padmanabhan, Sasisanker ^[1]; Schwyter, Philippe ^[2]; Liu, Zhongguo ^[2]; Poon, Geoffrey ^[2]; Bell, Alexis T. ^[2]; Prausnitz, John M. ^[2]

Univ. of California, Berkeley, CA (United States); Praj Industries, Pune (India)
Univ. of California, Berkeley, CA (United States)

Pretreatment of miscanthus is essential for efficient enzymatic production of cellulosic ethanol. This study reports a possible pretreatment method for miscanthus using aqueous ethylenediamine (EDA) for 30 min at 180 °C with or without ammonia. The mass ratio of miscanthus to EDA was varied from 1:3, 1:1, and 1:0.5, keeping the mass ratio of miscanthus to liquid (EDA + Water) constant at 1:8. The ammonia-to-miscanthus ratio was 1:0.25. After pretreatment with a ratio of 1:3 miscanthus to EDA, about 75 % of the lignin was removed from the raw miscanthus with 90 % retention of cellulose and 50 % of hemicellulose in the recovered solid. Enzymatic hydrolysis of the recovered solid miscanthus gave 63 % glucose and 62 % xylose conversion after 72 h. EDA provides an effective pretreatment for miscanthus, achieving good delignification and enhanced sugar yield by enzyme hydrolysis. Results using aqueous EDA with or without ammonia are much better than those using hot water and compare favorably with those using aqueous ammonia. The delignification efficiency of EDA pretreatment is high compared to that for hot-water pretreatment and is nearly as efficient as that obtained for aqueous-ammonia pretreatment.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1571069

Journal Information:: 3 Biotech, Journal Name: 3 Biotech Journal Issue: 1 Vol. 6; ISSN 2190-572X

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Investigation of biomass degradation mechanism in pretreatment of switchgrass by aqueous ammonia and sodium hydroxide Gupta, Rajesh; Lee, Y. Y. Bioresource Technology, Vol. 101, Issue 21 https://doi.org/10.1016/j.biortech.2010.05.039	journal	November 2010
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Aqueous-ammonia delignification of miscanthus followed by enzymatic hydrolysis to sugars Liu, Zhongguo; Padmanabhan, Sasisanker; Cheng, Kun Bioresource Technology, Vol. 135 https://doi.org/10.1016/j.biortech.2012.10.133	journal	May 2013
Biomass pretreatment: Fundamentals toward application Agbor, Valery B.; Cicek, Nazim; Sparling, Richard Biotechnology Advances, Vol. 29, Issue 6 https://doi.org/10.1016/j.biotechadv.2011.05.005	journal	November 2011
‘Cradle-to-grave’ assessment of existing lignocellulose pretreatment technologies da Costa Sousa, Leonardo; Chundawat, Shishir PS; Balan, Venkatesh Current Opinion in Biotechnology, Vol. 20, Issue 3 https://doi.org/10.1016/j.copbio.2009.05.003	journal	June 2009
Advances in ethanol production Geddes, Claudia C.; Nieves, Ismael U.; Ingram, Lonnie O. Current Opinion in Biotechnology, Vol. 22, Issue 3 https://doi.org/10.1016/j.copbio.2011.04.012	journal	June 2011
Solubility and rate of dissolution for Miscanthus in hydrophilic ionic liquids Padmanabhan, Sasisanker; Kim, Michael; Blanch, Harvey W. Fluid Phase Equilibria, Vol. 309, Issue 1 https://doi.org/10.1016/j.fluid.2011.06.034	journal	October 2011
Characterization of milled wood lignin and ethanol organosolv lignin from miscanthus El Hage, Roland; Brosse, Nicolas; Chrusciel, Laurent Polymer Degradation and Stability, Vol. 94, Issue 10 https://doi.org/10.1016/j.polymdegradstab.2009.07.007	journal	October 2009
Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J. Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729 https://doi.org/10.1021/Ie801542g	journal	April 2009
Pretreatment of Miscanthus x giganteus Using the Ethanol Organosolv Process for Ethanol Production Brosse, Nicolas; Sannigrahi, Poulomi; Ragauskas, Arthur Industrial & Engineering Chemistry Research, Vol. 48, Issue 18 https://doi.org/10.1021/Ie9006672	journal	September 2009
Compositional Analysis of Lignocellulosic Feedstocks. 1. Review and Description of Methods Sluiter, Justin B.; Ruiz, Raymond O.; Scarlata, Christopher J. Journal of Agricultural and Food Chemistry, Vol. 58, Issue 16, p. 9043-9053 https://doi.org/10.1021/Jf1008023	journal	August 2010
Compositional Analysis of Lignocellulosic Feedstocks. 2. Method Uncertainties Templeton, David W.; Scarlata, Christopher J.; Sluiter, Justin B. Journal of Agricultural and Food Chemistry, Vol. 58, Issue 16 https://doi.org/10.1021/Jf100807b	journal	August 2010
Plant cell walls to ethanol Jordan, Douglas B.; Bowman, Michael J.; Braker, Jay D. Biochemical Journal, Vol. 442, Issue 2 https://doi.org/10.1042/Bj20111922	journal	February 2012
Delignification of Miscanthus by Extraction Padmanabhan, Sasisanker; Zaia, Edmond; Wu, Katherine Separation Science and Technology, Vol. 47, Issue 2 https://doi.org/10.1080/01496395.2011.629639	journal	January 2012
Dissolution of Ethylenediamine Pretreated Pulp with High Lignin Content in LiCl/DMSO without Milling Wang, Zhiguo; Yokoyama, Tomoya; Matsumoto, Yuji Journal of Wood Chemistry and Technology, Vol. 30, Issue 3 https://doi.org/10.1080/02773810903477613	journal	August 2010
Cellulosic Biofuels Carroll, Andrew; Somerville, Chris Annual Review of Plant Biology, Vol. 60, Issue 1 https://doi.org/10.1146/annurev.arplant.043008.092125	journal	June 2009
Efficient hydrogen production from the lignocellulosic energy crop Miscanthus by the extreme thermophilic bacteria Caldicellulosiruptor saccharolyticus and Thermotoga neapolitana de Vrije, Truus; Bakker, Robert R.; Budde, Miriam AW Biotechnology for Biofuels, Vol. 2, Issue 1 https://doi.org/10.1186/1754-6834-2-12	journal	January 2009
Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover Qing, Qing; Wyman, Charles E. Biotechnology for Biofuels, Vol. 4, Issue 1, Article No: 18 https://doi.org/10.1186/1754-6834-4-18	journal	June 2011
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