How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

Chavent, Matthieu; Duncan, Anna L.; Rassam, Patrice; Birkholz, Oliver; Hélie, Jean; Reddy, Tyler John Edward; Beliaev, Dmitry; Hambly, Ben; Piehler, Jacob; Kleanthous, Colin; Sansom, Mark S. P.

doi:10.1038/s41467-018-05255-9

Title: How nanoscale protein interactions determine the mesoscale dynamic organisation of bacterial outer membrane proteins

Journal Article · 20 July 2018 · Nature Communications

DOI: https://doi.org/10.1038/s41467-018-05255-9 · OSTI ID:1570630

^[1]; Duncan, Anna L. ^[2]; Rassam, Patrice ^[3];

^[4];

^[5];

^[2]; Beliaev, Dmitry ^[6]; Hambly, Ben ^[7];

^[8];

^[2];

^[2]

Univ. of Oxford (United Kingdom); Univ. de Toulouse, Toulouse (France)
Univ. of Oxford (United Kingdom)
Univ. of Oxford (United Kingdom); Univ. de Strasbourg, Illkirch (France)
Univ. of Osnabrück, Osnabrück (Germany)
Univ. of Oxford (United Kingdom); SEMMLE, Oxford (United Kingdom)
Univ. of Oxford (United Kingdom); Radcliffe Observatory Quarter (550), Oxford (United Kingdom)
Radcliffe Observatory Quarter (550), Oxford (United Kingdom)
Univ. of Osnabrück, Barbarastraße (Osnabrück)

The spatiotemporal organisation of membranes is often characterised by the formation of large protein clusters. In Escherichia coli, outer membrane protein (OMP) clustering leads to OMP islands, the formation of which underpins OMP turnover and drives organisation across the cell envelope. Modelling how OMP islands form in order to understand their origin and outer membrane behaviour has been confounded by the inherent difficulties of simulating large numbers of OMPs over meaningful timescales. Here, we overcome these problems by training a mesoscale model incorporating thousands of OMPs on coarse-grained molecular dynamics simulations. We achieve simulations over timescales that allow direct comparison to experimental data of OMP behaviour. Here, we show that specific interaction surfaces between OMPs are key to the formation of OMP clusters, that OMP clusters present a mesh of moving barriers that confine newly inserted proteins within islands, and that mesoscale simulations recapitulate the restricted diffusion characteristics of OMPs.

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Research Organization:: Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: 89233218CNA000001

OSTI ID:: 1570630

Report Number(s):: LA-UR-19-29545

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 40 works

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