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Title: Imaging effectiveness calculator for non-design microscope samples

Abstract

When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Furthermore, we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.

Authors:
ORCiD logo [1];  [1]; ORCiD logo [1];  [1]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1570285
Alternate Identifier(s):
OSTI ID: 1544590
Report Number(s):
SAND-2019-11613J
Journal ID: ISSN 1559-128X; APOPAI; 679980
Grant/Contract Number:  
AC04-94AL85000; NA0003525
Resource Type:
Accepted Manuscript
Journal Name:
Applied Optics
Additional Journal Information:
Journal Volume: 58; Journal Issue: 22; Journal ID: ISSN 1559-128X
Publisher:
Optical Society of America
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Anthony, Stephen M., Miller, Philip R., Timlin, Jerilyn A., and Polsky, Ronen. Imaging effectiveness calculator for non-design microscope samples. United States: N. p., 2019. Web. https://doi.org/10.1364/AO.58.006027.
Anthony, Stephen M., Miller, Philip R., Timlin, Jerilyn A., & Polsky, Ronen. Imaging effectiveness calculator for non-design microscope samples. United States. https://doi.org/10.1364/AO.58.006027
Anthony, Stephen M., Miller, Philip R., Timlin, Jerilyn A., and Polsky, Ronen. Fri . "Imaging effectiveness calculator for non-design microscope samples". United States. https://doi.org/10.1364/AO.58.006027. https://www.osti.gov/servlets/purl/1570285.
@article{osti_1570285,
title = {Imaging effectiveness calculator for non-design microscope samples},
author = {Anthony, Stephen M. and Miller, Philip R. and Timlin, Jerilyn A. and Polsky, Ronen},
abstractNote = {When attempting to integrate single-molecule fluorescence microscopy with microfabricated devices such as microfluidic channels, fabrication constraints may prevent using traditional coverslips. Instead, the fabricated devices may require imaging through material with a different thickness or index of refraction. Altering either can easily reduce the quality of the image formation (measured by the Strehl ratio) by a factor of 2 or more, reducing the signal-to-noise ratio accordingly. In such cases, successful detection of single-molecule fluorescence may prove difficult or impossible. Furthermore, we provide software to calculate the effect of non-design materials upon the Strehl ratio or ensquared energy and explore the impact of common materials used in microfabrication.},
doi = {10.1364/AO.58.006027},
journal = {Applied Optics},
number = 22,
volume = 58,
place = {United States},
year = {2019},
month = {7}
}

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