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Title: Bioremediation of a Common Product of Food Processing by a Human Gut Bacterium

Abstract

Dramatic increases in processed food consumption represent a global health threat. Maillard reaction products (MRPs), which are common in processed foods, form upon heat-induced reaction of amino acids with reducing sugars and include advanced glycation end products with deleterious health effects. To examine how processed foods affect the microbiota, we fed gnotobiotic mice, colonized with 54 phylogenetically diverse human gut bacterial strains, defined sugar-rich diets containing whey as the protein source or a matched amino acid mixture. Whey or ϵ-fructoselysine, an MRP in whey and many processed foods, selectively increases Collinsella intestinalis absolute abundance and induces Collinsella expression of genomic loci directing import and metabolism of ϵ-fructoselysine to innocuous products. This locus is repressed by glucose in C. aerofaciens, whose abundance decreases with whey, but is not repressed in C. intestinalis. Finally, identifying gut organisms responding to and degrading potentially harmful processed food components has implications for food science, microbiome science, and public health.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]; ORCiD logo [2];  [2]; ORCiD logo [2];  [3];  [3];  [4];  [1]
  1. Washington Univ. School of Medicine, St. Louis, MO (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Russian Academy of Sciences (RAS), Moscow (Russian Federation); Sanford Burnham Prebys Medical Discovery Inst., La Jolla, CA (United States). Infectious and Inflammatory Disease Center
  4. Sanford Burnham Prebys Medical Discovery Inst., La Jolla, CA (United States). Infectious and Inflammatory Disease Center
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH); Russian Science Foundation
OSTI Identifier:
1570110
Grant/Contract Number:  
AC05-00OR22725; DK70977; DK078669; DK30292; 1-16-PDF-125
Resource Type:
Accepted Manuscript
Journal Name:
Cell Host & Microbe
Additional Journal Information:
Journal Volume: 26; Journal Issue: 4; Journal ID: ISSN 1931-3128
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wolf, Ashley R., Wesener, Darryl A., Cheng, Jiye, Houston-Ludlam, Alexandra N., Beller, Zachary W., Hibberd, Matthew C., Giannone, Richard J., Peters, Samantha L., Hettich, Robert L., Leyn, Semen A., Rodionov, Dmitry A., Osterman, Andrei L., and Gordon, Jeffrey I. Bioremediation of a Common Product of Food Processing by a Human Gut Bacterium. United States: N. p., 2019. Web. doi:10.1016/j.chom.2019.09.001.
Wolf, Ashley R., Wesener, Darryl A., Cheng, Jiye, Houston-Ludlam, Alexandra N., Beller, Zachary W., Hibberd, Matthew C., Giannone, Richard J., Peters, Samantha L., Hettich, Robert L., Leyn, Semen A., Rodionov, Dmitry A., Osterman, Andrei L., & Gordon, Jeffrey I. Bioremediation of a Common Product of Food Processing by a Human Gut Bacterium. United States. doi:10.1016/j.chom.2019.09.001.
Wolf, Ashley R., Wesener, Darryl A., Cheng, Jiye, Houston-Ludlam, Alexandra N., Beller, Zachary W., Hibberd, Matthew C., Giannone, Richard J., Peters, Samantha L., Hettich, Robert L., Leyn, Semen A., Rodionov, Dmitry A., Osterman, Andrei L., and Gordon, Jeffrey I. Tue . "Bioremediation of a Common Product of Food Processing by a Human Gut Bacterium". United States. doi:10.1016/j.chom.2019.09.001.
@article{osti_1570110,
title = {Bioremediation of a Common Product of Food Processing by a Human Gut Bacterium},
author = {Wolf, Ashley R. and Wesener, Darryl A. and Cheng, Jiye and Houston-Ludlam, Alexandra N. and Beller, Zachary W. and Hibberd, Matthew C. and Giannone, Richard J. and Peters, Samantha L. and Hettich, Robert L. and Leyn, Semen A. and Rodionov, Dmitry A. and Osterman, Andrei L. and Gordon, Jeffrey I.},
abstractNote = {Dramatic increases in processed food consumption represent a global health threat. Maillard reaction products (MRPs), which are common in processed foods, form upon heat-induced reaction of amino acids with reducing sugars and include advanced glycation end products with deleterious health effects. To examine how processed foods affect the microbiota, we fed gnotobiotic mice, colonized with 54 phylogenetically diverse human gut bacterial strains, defined sugar-rich diets containing whey as the protein source or a matched amino acid mixture. Whey or ϵ-fructoselysine, an MRP in whey and many processed foods, selectively increases Collinsella intestinalis absolute abundance and induces Collinsella expression of genomic loci directing import and metabolism of ϵ-fructoselysine to innocuous products. This locus is repressed by glucose in C. aerofaciens, whose abundance decreases with whey, but is not repressed in C. intestinalis. Finally, identifying gut organisms responding to and degrading potentially harmful processed food components has implications for food science, microbiome science, and public health.},
doi = {10.1016/j.chom.2019.09.001},
journal = {Cell Host & Microbe},
number = 4,
volume = 26,
place = {United States},
year = {2019},
month = {10}
}

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This content will become publicly available on October 1, 2020
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