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Title: Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach

Abstract

For decades entomopathogenic fungi have garnered interest as possible alternatives to chemical pesticides. However, their ecology outside of agroecosystems demands further study. We assessed the diversity and abundance of entomopathogenic and insect-associated fungi at a loblolly pine forest in North Carolina, USA using culture-dependent and next-generation sequencing libraries. Fungi were isolated using Galleria mellonella larvae, as well as from soil dilutions plated on a selective medium. Isolates were identified using Sanger sequencing of the ITS and LSU rRNA gene regions, and represented 36 OTUs including Metarhizium, Lecanicillium, and Paecilomyces. Additionally, we assessed the chitinolytic potential of isolates and found widespread, variable ability to degrade chitin within and between genera. Phylogenetic analyses resolved several isolates to genus, with some forming clades with other insect-associated taxa, as well as with fungi associated with plant tissues. Saprophytes were widely distributed in soil, while entomopathogens were less abundant and present primarily in the top two cm of the soil. Furthermore, the similarity between culture-dependent and next-generation sequencing results demonstrates that both methods can be used concurrently in this system to study the ecology of entomopathogenic fungi.

Authors:
 [1];  [2]; ORCiD logo [3]; ORCiD logo [1]
  1. Western Illinois Univ., Macomb, IL (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); United States Department of Agriculture, Corvallis, OR (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1569616
Report Number(s):
LA-UR-19-20465
Journal ID: ISSN 1878-6146
Grant/Contract Number:  
89233218CNA000001
Resource Type:
Accepted Manuscript
Journal Name:
Fungal Biology
Additional Journal Information:
Journal Name: Fungal Biology; Journal ID: ISSN 1878-6146
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
Biological Science; Chitin; Next generation sequencing; Selective media; Soil fungi

Citation Formats

Deaver, Noland R., Hesse, Cedar, Kuske, Cheryl Rae, and Porras-Alfaro, Andrea. Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach. United States: N. p., 2019. Web. doi:10.1016/j.funbio.2019.09.006.
Deaver, Noland R., Hesse, Cedar, Kuske, Cheryl Rae, & Porras-Alfaro, Andrea. Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach. United States. doi:10.1016/j.funbio.2019.09.006.
Deaver, Noland R., Hesse, Cedar, Kuske, Cheryl Rae, and Porras-Alfaro, Andrea. Mon . "Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach". United States. doi:10.1016/j.funbio.2019.09.006.
@article{osti_1569616,
title = {Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach},
author = {Deaver, Noland R. and Hesse, Cedar and Kuske, Cheryl Rae and Porras-Alfaro, Andrea},
abstractNote = {For decades entomopathogenic fungi have garnered interest as possible alternatives to chemical pesticides. However, their ecology outside of agroecosystems demands further study. We assessed the diversity and abundance of entomopathogenic and insect-associated fungi at a loblolly pine forest in North Carolina, USA using culture-dependent and next-generation sequencing libraries. Fungi were isolated using Galleria mellonella larvae, as well as from soil dilutions plated on a selective medium. Isolates were identified using Sanger sequencing of the ITS and LSU rRNA gene regions, and represented 36 OTUs including Metarhizium, Lecanicillium, and Paecilomyces. Additionally, we assessed the chitinolytic potential of isolates and found widespread, variable ability to degrade chitin within and between genera. Phylogenetic analyses resolved several isolates to genus, with some forming clades with other insect-associated taxa, as well as with fungi associated with plant tissues. Saprophytes were widely distributed in soil, while entomopathogens were less abundant and present primarily in the top two cm of the soil. Furthermore, the similarity between culture-dependent and next-generation sequencing results demonstrates that both methods can be used concurrently in this system to study the ecology of entomopathogenic fungi.},
doi = {10.1016/j.funbio.2019.09.006},
journal = {Fungal Biology},
number = ,
volume = ,
place = {United States},
year = {2019},
month = {9}
}

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This content will become publicly available on September 23, 2020
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