skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach

Abstract

For decades entomopathogenic fungi have garnered interest as possible alternatives to chemical pesticides. However, their ecology outside of agroecosystems demands further study. We assessed the diversity and abundance of entomopathogenic and insect-associated fungi at a loblolly pine forest in North Carolina, USA using culture-dependent and next-generation sequencing libraries. Fungi were isolated using Galleria mellonella larvae, as well as from soil dilutions plated on a selective medium. Isolates were identified using Sanger sequencing of the ITS and LSU rRNA gene regions, and represented 36 OTUs including Metarhizium, Lecanicillium, and Paecilomyces. Additionally, we assessed the chitinolytic potential of isolates and found widespread, variable ability to degrade chitin within and between genera. Phylogenetic analyses resolved several isolates to genus, with some forming clades with other insect-associated taxa, as well as with fungi associated with plant tissues. Saprophytes were widely distributed in soil, while entomopathogens were less abundant and present primarily in the top two cm of the soil. Furthermore, the similarity between culture-dependent and next-generation sequencing results demonstrates that both methods can be used concurrently in this system to study the ecology of entomopathogenic fungi.

Authors:
 [1];  [2]; ORCiD logo [3]; ORCiD logo [1]
  1. Western Illinois Univ., Macomb, IL (United States)
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States); United States Department of Agriculture, Corvallis, OR (United States)
  3. Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1569616
Alternate Identifier(s):
OSTI ID: 1780389
Report Number(s):
LA-UR-19-20465
Journal ID: ISSN 1878-6146
Grant/Contract Number:  
89233218CNA000001
Resource Type:
Accepted Manuscript
Journal Name:
Fungal Biology
Additional Journal Information:
Journal Volume: 123; Journal Issue: 12; Journal ID: ISSN 1878-6146
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Biological Science; Chitin; Next generation sequencing; Selective media; Soil fungi

Citation Formats

Deaver, Noland R., Hesse, Cedar, Kuske, Cheryl Rae, and Porras-Alfaro, Andrea. Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach. United States: N. p., 2019. Web. doi:10.1016/j.funbio.2019.09.006.
Deaver, Noland R., Hesse, Cedar, Kuske, Cheryl Rae, & Porras-Alfaro, Andrea. Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach. United States. doi:https://doi.org/10.1016/j.funbio.2019.09.006
Deaver, Noland R., Hesse, Cedar, Kuske, Cheryl Rae, and Porras-Alfaro, Andrea. Mon . "Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach". United States. doi:https://doi.org/10.1016/j.funbio.2019.09.006. https://www.osti.gov/servlets/purl/1569616.
@article{osti_1569616,
title = {Presence and distribution of insect-associated and entomopathogenic fungi in a temperate pine forest soil: An integrated approach},
author = {Deaver, Noland R. and Hesse, Cedar and Kuske, Cheryl Rae and Porras-Alfaro, Andrea},
abstractNote = {For decades entomopathogenic fungi have garnered interest as possible alternatives to chemical pesticides. However, their ecology outside of agroecosystems demands further study. We assessed the diversity and abundance of entomopathogenic and insect-associated fungi at a loblolly pine forest in North Carolina, USA using culture-dependent and next-generation sequencing libraries. Fungi were isolated using Galleria mellonella larvae, as well as from soil dilutions plated on a selective medium. Isolates were identified using Sanger sequencing of the ITS and LSU rRNA gene regions, and represented 36 OTUs including Metarhizium, Lecanicillium, and Paecilomyces. Additionally, we assessed the chitinolytic potential of isolates and found widespread, variable ability to degrade chitin within and between genera. Phylogenetic analyses resolved several isolates to genus, with some forming clades with other insect-associated taxa, as well as with fungi associated with plant tissues. Saprophytes were widely distributed in soil, while entomopathogens were less abundant and present primarily in the top two cm of the soil. Furthermore, the similarity between culture-dependent and next-generation sequencing results demonstrates that both methods can be used concurrently in this system to study the ecology of entomopathogenic fungi.},
doi = {10.1016/j.funbio.2019.09.006},
journal = {Fungal Biology},
number = 12,
volume = 123,
place = {United States},
year = {2019},
month = {9}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 2 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Beauveria bassiana (Balsamo) Vuillemin as an endophyte in tissue culture banana (Musa spp.)
journal, September 2007

  • Akello, Juliet; Dubois, Thomas; Gold, Clifford S.
  • Journal of Invertebrate Pathology, Vol. 96, Issue 1
  • DOI: 10.1016/j.jip.2007.02.004

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
  • DOI: 10.1016/S0022-2836(05)80360-2

Diversity and phylogenetic affinities of foliar fungal endophytes in loblolly pine inferred by culturing and environmental PCR
journal, March 2007


Distribution of Chitinases in the Entomopathogen Metarhizium anisopliae and Effect of N -Acetylglucosamine in Protein Secretion
journal, February 2004

  • Barreto, Cristine Chaves; Staats, Charley Christian; Schrank, Augusto
  • Current Microbiology, Vol. 48, Issue 2
  • DOI: 10.1007/s00284-003-4063-z

Habitat Association in Two Genetic Groups of the Insect-Pathogenic Fungus Metarhizium anisopliae: Uncovering Cryptic Species?
journal, March 2001

  • Bidochka, Michael J.; Kamp, Andrena M.; Lavender, T. Michael
  • Applied and Environmental Microbiology, Vol. 67, Issue 3
  • DOI: 10.1128/AEM.67.3.1335-1342.2001

Genetic groups of the insect-pathogenic fungus Beauveria bassiana are associated with habitat and thermal growth preferences
journal, December 2002

  • Bidochka, Michael; Menzies, Fiona; Kamp, Andrena
  • Archives of Microbiology, Vol. 178, Issue 6
  • DOI: 10.1007/s00203-002-0490-7

Sampling and occurrence of entomopathogenic fungi and nematodes in UK soils
journal, May 1997


Isolation and characterization of three chitinases from Trichoderma harzianum
journal, June 1992


Carbon source control on β-glucanases, chitobiase and chitinase from Trichoderma harzianum
journal, April 1993

  • de la Cruz, Jesús; Rey, Manuel; Lora, José M.
  • Archives of Microbiology, Vol. 159, Issue 4
  • DOI: 10.1007/BF00290913

Review of Fungal Chitinases
journal, June 2006


CTC medium: A novel dodine-free selective medium for isolating entomopathogenic fungi, especially Metarhizium acridum, from soil
journal, September 2010


Insecticide Resistance Signals Negative Consequences of Widespread Neonicotinoid Use on Multiple Field Crops in the U.S. Cotton Belt
journal, January 2018

  • Huseth, Anders S.; Chappell, Thomas M.; Chitturi, Anitha
  • Environmental Science & Technology, Vol. 52, Issue 4
  • DOI: 10.1021/acs.est.7b06015

Effects of farming system, field margins and bait insect on the occurrence of insect pathogenic fungi in soils
journal, September 2002

  • Klingen, Ingeborg; Eilenberg, Jørgen; Meadow, Richard
  • Agriculture, Ecosystems & Environment, Vol. 91, Issue 1-3
  • DOI: 10.1016/S0167-8809(01)00227-4

Assembling the fungal tree of life: progress, classification, and evolution of subcellular traits
journal, October 2004

  • Lutzoni, François; Kauff, Frank; Cox, Cymon J.
  • American Journal of Botany, Vol. 91, Issue 10
  • DOI: 10.3732/ajb.91.10.1446

Occurrence and distribution of soil borne entomopathogenic fungi within a single organic agroecosystem
journal, April 2006

  • Meyling, Nicolai V.; Eilenberg, Jørgen
  • Agriculture, Ecosystems & Environment, Vol. 113, Issue 1-4
  • DOI: 10.1016/j.agee.2005.10.011

Fungal Community Analysis by Large-Scale Sequencing of Environmental Samples
journal, September 2005


Evolutionary relationships among mucoralean fungi (Zygomycota): Evidence for family polyphyly on a large scale
journal, March 2001


Chitinolytic enzymes: an exploration
journal, April 2000

  • Patil, Reetarani S.; Ghormade, Vandana; Deshpande, Mukund V.
  • Enzyme and Microbial Technology, Vol. 26, Issue 7
  • DOI: 10.1016/S0141-0229(00)00134-4

Diversity and distribution of soil fungal communities in a semiarid grassland
journal, January 2011

  • Porras-Alfaro, Andrea; Herrera, Jose; Natvig, Donald O.
  • Mycologia, Vol. 103, Issue 1
  • DOI: 10.3852/09-297

Factors affecting the occurrence and distribution of entomopathogenic fungi in natural and cultivated soils
journal, August 2007

  • Quesada-Moraga, Enrique; Navas-Cortés, Juan A.; Maranhao, Elizabeth A. A.
  • Mycological Research, Vol. 111, Issue 8
  • DOI: 10.1016/j.mycres.2007.06.006

Molecular systematics of the Hypocreales: a teleomorph gene phylogeny and the status of their anamorphs
journal, December 1995

  • Rehner, Stephen A.; Samuels, Gary J.
  • Canadian Journal of Botany, Vol. 73, Issue S1
  • DOI: 10.1139/b95-327

The contribution of agricultural insecticide use to increasing insecticide resistance in African malaria vectors
journal, February 2016


Plant and Bacterial Chitinases Differ in Antifungal Activity
journal, January 1988


Entomopathogenic fungi in cornfields and their potential to manage larval western corn rootworm Diabrotica virgifera virgifera
journal, November 2013

  • Rudeen, Melissa L.; Jaronski, Stefan T.; Petzold-Maxwell, Jennifer L.
  • Journal of Invertebrate Pathology, Vol. 114, Issue 3
  • DOI: 10.1016/j.jip.2013.09.009

Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi
journal, March 2012

  • Schoch, C. L.; Seifert, K. A.; Huhndorf, S.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 16
  • DOI: 10.1073/pnas.1117018109

Entomopathogenic Isolates of Metarhizium anisopliae, Beauveria bassiana, and Aspergillus flavus Produce Multiple Extracellular Chitinase Isozymes
journal, January 1993

  • St. Leger, Raymond J.; Staples, Richard C.; Roberts, Donald W.
  • Journal of Invertebrate Pathology, Vol. 61, Issue 1
  • DOI: 10.1006/jipa.1993.1014

Occurrence and diversity of insect-associated fungi in natural soils in China
journal, May 2008


Insect-associated fungi in soils of field crops and orchards
journal, November 2008


MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods
journal, May 2011

  • Tamura, K.; Peterson, D.; Peterson, N.
  • Molecular Biology and Evolution, Vol. 28, Issue 10
  • DOI: 10.1093/molbev/msr121

Study on the communities of endophytic fungi and endophytic actinomycetes from rice and their antipathogenic activities in vitro
journal, January 2004


Presence and distribution of heavy metal tolerant fungi in surface soils of a temperate pine forest
journal, October 2018


Drivers of entomopathogenic fungi presence in organic and conventional vineyard soils
journal, January 2019


Entomopathogenic fungal endophytes
journal, July 2008


Fungal entomopathogens: new insights on their ecology
journal, November 2009


Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species.
journal, August 1990


Colonization of Corn, Zea mays, by the Entomopathogenic Fungus Beauveria bassiana
journal, August 2000


A comprehensive molecular phylogeny of the <I>Mortierellales</I> (<I>Mortierellomycotina</I>) based on nuclear ribosomal DNA
journal, June 2013

  • Wagner, L.; Stielow, B.; Hoffmann, K.
  • Persoonia - Molecular Phylogeny and Evolution of Fungi, Vol. 30, Issue 1
  • DOI: 10.3767/003158513X666268

Naive Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial Taxonomy
journal, June 2007

  • Wang, Q.; Garrity, G. M.; Tiedje, J. M.
  • Applied and Environmental Microbiology, Vol. 73, Issue 16
  • DOI: 10.1128/AEM.00062-07

Phylogeny of the Zygomycota based on nuclear ribosomal sequence data
journal, November 2006


An overview of the systematics of the Sordariomycetes based on a four-gene phylogeny
journal, November 2006