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Title: The Salix psammophila SpRLCK1 involved in drought and salt tolerance

Abstract

Receptor-like cytoplasmic kinases ( RLCKs) play critical roles in biotic and abiotic stress responses in plants. However, the functions of RLCKs from the desert shrub willow Salix psammophila have not been characterized. Here, we focused on the biological function of SpRLCK1, which was previously identified as a potential drought-related gene. Phylogenetic analysis and subcellular localization revealed that SpRLCK1 was a cytoplasmic-localized protein with a protein kinase domain and belonged to the RLCK VIIa subclass. Gene expression profile revealed that SpRLCK1 was predominantly expressed in the root, being consistent with the GUS staining of pSpRLCK1:GUS transgenic plants. Additionally, the expression of SpRLCK1 was significantly induced by drought and salt stresses. To verify the function of SpRLCK1, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The SpRLCK1-overexpressing plants exhibited higher tolerance to drought and salt stresses, as evidenced by the higher survival rate, relative water content and antioxidant enzyme activity than those of wild-type plants. The SpRLCK1-overexpressing plants enhanced drought and salt tolerance by improving ROS-scavenging activities. A co-expression network for SpRLCK1 was constructed, and the expression analysis indicated that SpRLCK1 regulated the expression of a series of stress-related genes. Ultimately, our results demonstrate that SpRLCK1 confers plant drought and saltmore » tolerance through enhancing the activity of antioxidant enzymes and cooperating with stress-related genes.« less

Authors:
 [1]; ORCiD logo [2];  [3];  [1];  [1]; ORCiD logo [1]
  1. Chinese Academy of Forestry, Beijing (China). Research Inst. of Forestry, Key Lab. of Tree Breeding and Cultivation of the National Forestry and Grassland Administration, State Key Lab. of Tree Genetics and Breeding
  2. Chinese Academy of Forestry, Beijing (China). Experimental Center of Forestry in North China
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biosciences Division
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1569361
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Plant Physiology and Biochemistry
Additional Journal Information:
Journal Volume: 144; Journal Issue: C; Journal ID: ISSN 0981-9428
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; receptor-like protein kinase; transgene; drought; salt; antioxidant enzyme; Salix psammophila

Citation Formats

Jia, Huixia, Li, Jianbo, Zhang, Jin, Sun, Pei, Lu, Mengzhu, and Hu, Jianjun. The Salix psammophila SpRLCK1 involved in drought and salt tolerance. United States: N. p., 2019. Web. doi:10.1016/j.plaphy.2019.09.042.
Jia, Huixia, Li, Jianbo, Zhang, Jin, Sun, Pei, Lu, Mengzhu, & Hu, Jianjun. The Salix psammophila SpRLCK1 involved in drought and salt tolerance. United States. doi:10.1016/j.plaphy.2019.09.042.
Jia, Huixia, Li, Jianbo, Zhang, Jin, Sun, Pei, Lu, Mengzhu, and Hu, Jianjun. Fri . "The Salix psammophila SpRLCK1 involved in drought and salt tolerance". United States. doi:10.1016/j.plaphy.2019.09.042.
@article{osti_1569361,
title = {The Salix psammophila SpRLCK1 involved in drought and salt tolerance},
author = {Jia, Huixia and Li, Jianbo and Zhang, Jin and Sun, Pei and Lu, Mengzhu and Hu, Jianjun},
abstractNote = {Receptor-like cytoplasmic kinases (RLCKs) play critical roles in biotic and abiotic stress responses in plants. However, the functions of RLCKs from the desert shrub willow Salix psammophila have not been characterized. Here, we focused on the biological function of SpRLCK1, which was previously identified as a potential drought-related gene. Phylogenetic analysis and subcellular localization revealed that SpRLCK1 was a cytoplasmic-localized protein with a protein kinase domain and belonged to the RLCK VIIa subclass. Gene expression profile revealed that SpRLCK1 was predominantly expressed in the root, being consistent with the GUS staining of pSpRLCK1:GUS transgenic plants. Additionally, the expression of SpRLCK1 was significantly induced by drought and salt stresses. To verify the function of SpRLCK1, we generated its overexpressing transgenic lines in Arabidopsis thaliana. The SpRLCK1-overexpressing plants exhibited higher tolerance to drought and salt stresses, as evidenced by the higher survival rate, relative water content and antioxidant enzyme activity than those of wild-type plants. The SpRLCK1-overexpressing plants enhanced drought and salt tolerance by improving ROS-scavenging activities. A co-expression network for SpRLCK1 was constructed, and the expression analysis indicated that SpRLCK1 regulated the expression of a series of stress-related genes. Ultimately, our results demonstrate that SpRLCK1 confers plant drought and salt tolerance through enhancing the activity of antioxidant enzymes and cooperating with stress-related genes.},
doi = {10.1016/j.plaphy.2019.09.042},
journal = {Plant Physiology and Biochemistry},
number = C,
volume = 144,
place = {United States},
year = {2019},
month = {11}
}

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