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Title: Fixation of CO 2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy

Abstract

Biological CO 2 fixation is an important technology that can assist in combating climate change. Here, we show an approach called anaerobic, non-photosynthetic mixotrophy can result in net CO 2 fixation when using a reduced feedstock. This approach uses microbes called acetogens that are capable of concurrent utilization of both organic and inorganic substrates. In this study, we investigated the substrate utilization of 17 different acetogens, both mesophilic and thermophilic, on a variety of different carbohydrates and gases. Compared to most model acetogen strains, several non-model mesophilic strains displayed greater substrate flexibility, including the ability to utilize disaccharides, glycerol and an oligosaccharide, and growth rates. Three of these non-model strains ( Blautia producta, Clostridium scatologenes and Thermoanaerobacter kivui) were chosen for further characterization, under a variety of conditions including H 2- or syngas-fed sugar fermentations and a CO 2-fed glycerol fermentation. In all cases, CO 2 was fixed and carbon yields approached 100%. Finally, the model acetogen C. ljungdahlii was engineered to utilize glucose, a non-preferred sugar, while maintaining mixotrophic behavior. This work demonstrates the flexibility and robustness of anaerobic, non-photosynthetic mixotrophy as a technology to help reduce CO 2 emissions.

Authors:
 [1];  [1];  [2];  [1];  [1]
  1. White Dog Labs, Inc., New Castle, DE (United States)
  2. CelDezyner, Rehovot (Israel)
Publication Date:
Research Org.:
White Dog Labs, Inc., New Castle, DE (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)
OSTI Identifier:
1569044
Grant/Contract Number:  
EE0007564
Resource Type:
Accepted Manuscript
Journal Name:
FEMS Microbiology Letters (Online)
Additional Journal Information:
Journal Name: FEMS Microbiology Letters (Online); Journal Volume: 365; Journal Issue: 8; Journal ID: ISSN 1574-6968
Publisher:
Federation of European Microbiological Societies
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; acetogen; mixotrophy; CO2 fixation; Wood–Ljungdahl pathway; glucose utilization; syngas

Citation Formats

Maru, Biniam T., Munasinghe, Pradeep C., Gilary, Hadar, Jones, Shawn W., and Tracy, Bryan P. Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy. United States: N. p., 2018. Web. doi:10.1093/femsle/fny039.
Maru, Biniam T., Munasinghe, Pradeep C., Gilary, Hadar, Jones, Shawn W., & Tracy, Bryan P. Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy. United States. doi:10.1093/femsle/fny039.
Maru, Biniam T., Munasinghe, Pradeep C., Gilary, Hadar, Jones, Shawn W., and Tracy, Bryan P. Fri . "Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy". United States. doi:10.1093/femsle/fny039. https://www.osti.gov/servlets/purl/1569044.
@article{osti_1569044,
title = {Fixation of CO2 and CO on a diverse range of carbohydrates using anaerobic, non-photosynthetic mixotrophy},
author = {Maru, Biniam T. and Munasinghe, Pradeep C. and Gilary, Hadar and Jones, Shawn W. and Tracy, Bryan P.},
abstractNote = {Biological CO2 fixation is an important technology that can assist in combating climate change. Here, we show an approach called anaerobic, non-photosynthetic mixotrophy can result in net CO2 fixation when using a reduced feedstock. This approach uses microbes called acetogens that are capable of concurrent utilization of both organic and inorganic substrates. In this study, we investigated the substrate utilization of 17 different acetogens, both mesophilic and thermophilic, on a variety of different carbohydrates and gases. Compared to most model acetogen strains, several non-model mesophilic strains displayed greater substrate flexibility, including the ability to utilize disaccharides, glycerol and an oligosaccharide, and growth rates. Three of these non-model strains (Blautia producta, Clostridium scatologenes and Thermoanaerobacter kivui) were chosen for further characterization, under a variety of conditions including H2- or syngas-fed sugar fermentations and a CO2-fed glycerol fermentation. In all cases, CO2 was fixed and carbon yields approached 100%. Finally, the model acetogen C. ljungdahlii was engineered to utilize glucose, a non-preferred sugar, while maintaining mixotrophic behavior. This work demonstrates the flexibility and robustness of anaerobic, non-photosynthetic mixotrophy as a technology to help reduce CO2 emissions.},
doi = {10.1093/femsle/fny039},
journal = {FEMS Microbiology Letters (Online)},
number = 8,
volume = 365,
place = {United States},
year = {2018},
month = {2}
}

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