Consistent production of chlorine-stressed bacteria from non-chlorinated secondary sewage effluents for use in the U.S. Environmental Protection Agency Alternate Test Procedure protocol

Journal Article · 07 June 2019 · Journal of Microbiological Methods

DOI: https://doi.org/10.1016/j.mimet.2019.105651 · OSTI ID:1568064

Hutchison, Janine R. ^[1]; Widder, Mark W. ^[2]; Brooks, Shelby M. ^[1]; Brennan, Linda M. ^[2]; Souris, Leah ^[2]; Divito, Valerie T. ^[2]; van der Schalie, William H. ^[2]; Ozanich, Richard M. ^[1]

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1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States). National Security Directorate, Chemical and Biological Signature Sciences Group
2. US Army Center for Environmental Health Research, Fort Detrick, MD (United States)

The U.S. Environmental Protection Agency Alternative Test Procedure protocol outlines a method to produce chlorine-stressed bacteria for water quality testing. Achieving consistent results is challenging due effluent variability. We describe methods for generating chlorine-stressed total coliforms from secondary effluent to evaluate detection technologies to demonstrate comparability to EPA reference methods

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• Grant/Contract Number: AC05-76RL01830
• OSTI ID: 1568064
• Report Number(s): PNNL-SA-142969
• Journal Information: Journal of Microbiological Methods, Vol. 163, Issue C; ISSN 0167-7012
• Publisher: ElsevierCopyright Statement
• Country of Publication: United States
• Language: English