Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors

Carper, Dana L.; Carrell, Alyssa A.; Kueppers, Lara M.; Frank, A. Carolin

doi:10.1007/s11104-018-3682-x

Title: Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors

Journal Article · 23 May 2018 · Plant and Soil

DOI: https://doi.org/10.1007/s11104-018-3682-x · OSTI ID:1567127

Carper, Dana L. ^[1]; Carrell, Alyssa A. ^[2]; Kueppers, Lara M. ^[3]; Frank, A. Carolin ^[1]

Univ. of California Merced, CA (United States)
Univ. of California Merced, CA (United States); Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Univ. of California Merced, CA (United States); Univ. of California, Berkeley, CA (United States)

Background and aims: Forest tree microbiomes are vital to forest dynamics, diversity, and ecosystem processes. Mature limber pines (Pinus flexilis) host a core microbiome of acetic acid bacteria in their foliage, but the bacterial endophyte community structure, variation, and assembly across tree ontogeny is unknown. The aims of this research were to test if the core microbiome observed in adult P. flexilis is established at the seedling stage, if seedlings host different endophyte communities in root and shoot tissues, and how environmental factors structure seedling endophyte communities. Methods: The 16S rRNA gene was sequenced to characterize the bacterial endophyte communities in roots and shoots of P. flexilis seedlings grown in plots at three elevations at Niwot Ridge, Colorado, subjected to experimental treatments (watering and heating). The data was compared to previously sequenced endophyte communities from adult tree foliage sampled in the same year and location. Results: Seedling shoots hosted a different core microbiome than adult tree foliage and were dominated by a few OTUs in the family Oxalobacteraceae, identical or closely related to strains with antifungal activity. Shoot and root communities significantly differed from each other but shared major OTUs. Watering but not warming restructured the seedling endophyte communities. Conclusions: The results indicate differences in assembly and ecological function across conifer life stages. Seedlings may recruit endophytes to protect against fungi under increased soil moisture.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF); National Institutes of Health (NIH)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1567127

Journal Information:: Plant and Soil, Vol. 428, Issue 1-2; ISSN 0032-079X

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 24 works

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Succession of endophytic fungi and arbuscular mycorrhizal fungi associated with the growth of plant and their correlation with secondary metabolites in the roots of plants Dang, Hanli; Zhang, Tao; Wang, Zhongke BMC Plant Biology, Vol. 21, Issue 1 https://doi.org/10.1186/s12870-021-02942-6	journal	April 2021
Host genotype and age shape the leaf and root microbiomes of a wild perennial plant S., Lundberg, Derek; L., Dangl, Jeffery; G., Del Rio, Tijana The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/18y1-ek17	text	January 2016
Root microbiota drive direct integration of phosphate stress and immunity W., Breakfield, Natalie; Gabriel, Castrillo,; F., Law, Theresa The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/a7y1-dm85	text	January 2017
Defining the core Arabidopsis thaliana root microbiome E., Ley, Ruth; Julien, Tremblay,; L., Dangl, Jeffery The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/w03e-5q36	text	January 2012
Oxalotrophy, a widespread trait of plant-associated Burkholderia species, is involved in successful root colonization of lupin and maize by Burkholderia phytofirmans Kost, Thomas; Stopnisek, Nejc; Agnoli, Kirsty ETH Zurich https://doi.org/10.3929/ethz-b-000116775	text	January 2014
Bacterial Communities Associated with the Leaves and the Roots of Arabidopsis thaliana Bodenhausen, Natacha; Horton, Matthew W.; Bergelson, Joy ETH Zurich https://doi.org/10.3929/ethz-b-000075849	text	January 2013
Diversity and heritability of the maize rhizosphere microbiome under field conditions Z., Jin,; S., Buckler, E.; O., Koren, The University of North Carolina at Chapel Hill University Libraries https://doi.org/10.17615/rzga-0c56	text	January 2013
Oxalotrophy, a widespread trait of plant-associated Burkholderia species, is involved in successful root colonization of lupin and maize by Burkholderia phytofirmans Kost, Thomas; Stopnisek, Nejc; Agnoli, Kirsty Frontiers Research Foundation https://doi.org/10.5167/uzh-105323	text	January 2014
Data from: The unique ecology of human predators Darimont, Chris T.; Fox, Caroline H.; Bryan, Heather M. Dryad https://doi.org/10.5061/dryad.238b2	dataset	January 2016

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Assembly of root-associated microbial community of typical rice cultivars in different soil types Xu, Yan; Ge, Yi; Song, Jianxiao Biology and Fertility of Soils, Vol. 56, Issue 2 https://doi.org/10.1007/s00374-019-01406-2	journal	December 2019