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Title: Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors

Abstract

Background and aims: Forest tree microbiomes are vital to forest dynamics, diversity, and ecosystem processes. Mature limber pines ( Pinus flexilis) host a core microbiome of acetic acid bacteria in their foliage, but the bacterial endophyte community structure, variation, and assembly across tree ontogeny is unknown. The aims of this research were to test if the core microbiome observed in adult P. flexilis is established at the seedling stage, if seedlings host different endophyte communities in root and shoot tissues, and how environmental factors structure seedling endophyte communities. Methods: The 16S rRNA gene was sequenced to characterize the bacterial endophyte communities in roots and shoots of P. flexilis seedlings grown in plots at three elevations at Niwot Ridge, Colorado, subjected to experimental treatments (watering and heating). The data was compared to previously sequenced endophyte communities from adult tree foliage sampled in the same year and location. Results: Seedling shoots hosted a different core microbiome than adult tree foliage and were dominated by a few OTUs in the family Oxalobacteraceae, identical or closely related to strains with antifungal activity. Shoot and root communities significantly differed from each other but shared major OTUs. Watering but not warming restructured the seedling endophyte communities.more » Conclusions: The results indicate differences in assembly and ecological function across conifer life stages. Seedlings may recruit endophytes to protect against fungi under increased soil moisture.« less

Authors:
 [1];  [2];  [3];  [1]
  1. Univ. of California Merced, CA (United States)
  2. Univ. of California Merced, CA (United States); Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Univ. of California Merced, CA (United States); Univ. of California, Berkeley, CA (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Science Foundation (NSF); National Institutes of Health (NIH)
OSTI Identifier:
1567127
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Plant and Soil
Additional Journal Information:
Journal Volume: 428; Journal Issue: 1-2; Journal ID: ISSN 0032-079X
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
16S rRNA; Endophytic bacteria; Pinus flexilis; Climate change; Conifers

Citation Formats

Carper, Dana L., Carrell, Alyssa A., Kueppers, Lara M., and Frank, A. Carolin. Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors. United States: N. p., 2018. Web. doi:10.1007/s11104-018-3682-x.
Carper, Dana L., Carrell, Alyssa A., Kueppers, Lara M., & Frank, A. Carolin. Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors. United States. doi:10.1007/s11104-018-3682-x.
Carper, Dana L., Carrell, Alyssa A., Kueppers, Lara M., and Frank, A. Carolin. Wed . "Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors". United States. doi:10.1007/s11104-018-3682-x. https://www.osti.gov/servlets/purl/1567127.
@article{osti_1567127,
title = {Bacterial endophyte communities in Pinus flexilis are structured by host age, tissue type, and environmental factors},
author = {Carper, Dana L. and Carrell, Alyssa A. and Kueppers, Lara M. and Frank, A. Carolin},
abstractNote = {Background and aims: Forest tree microbiomes are vital to forest dynamics, diversity, and ecosystem processes. Mature limber pines (Pinus flexilis) host a core microbiome of acetic acid bacteria in their foliage, but the bacterial endophyte community structure, variation, and assembly across tree ontogeny is unknown. The aims of this research were to test if the core microbiome observed in adult P. flexilis is established at the seedling stage, if seedlings host different endophyte communities in root and shoot tissues, and how environmental factors structure seedling endophyte communities. Methods: The 16S rRNA gene was sequenced to characterize the bacterial endophyte communities in roots and shoots of P. flexilis seedlings grown in plots at three elevations at Niwot Ridge, Colorado, subjected to experimental treatments (watering and heating). The data was compared to previously sequenced endophyte communities from adult tree foliage sampled in the same year and location. Results: Seedling shoots hosted a different core microbiome than adult tree foliage and were dominated by a few OTUs in the family Oxalobacteraceae, identical or closely related to strains with antifungal activity. Shoot and root communities significantly differed from each other but shared major OTUs. Watering but not warming restructured the seedling endophyte communities. Conclusions: The results indicate differences in assembly and ecological function across conifer life stages. Seedlings may recruit endophytes to protect against fungi under increased soil moisture.},
doi = {10.1007/s11104-018-3682-x},
journal = {Plant and Soil},
number = 1-2,
volume = 428,
place = {United States},
year = {2018},
month = {5}
}

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Works referenced in this record:

Basic local alignment search tool
journal, October 1990

  • Altschul, Stephen F.; Gish, Warren; Miller, Webb
  • Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
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