Interconnection of Key Microbial Functional Genes for Enhanced Benzo[a]pyrene Biodegradation in Sediments by Microbial Electrochemistry

Yan, Zaisheng; He, Yuhong; Cai, Haiyuan; Van Nostrand, Joy D.; He, Zhili; Zhou, Jizhong; Krumholz, Lee R.; Jiang, He-Long

doi:10.1021/acs.est.7b00209

Title: Interconnection of Key Microbial Functional Genes for Enhanced Benzo[a]pyrene Biodegradation in Sediments by Microbial Electrochemistry

Journal Article · 05 July 2017 · Environmental Science and Technology

DOI: https://doi.org/10.1021/acs.est.7b00209 · OSTI ID:1567094

Yan, Zaisheng ^[1]; He, Yuhong ^[1]; Cai, Haiyuan ^[1]; Van Nostrand, Joy D. ^[2]; He, Zhili ^[2]; Zhou, Jizhong ^[3]; Krumholz, Lee R. ^[2];

^[1]

Chinese Academy of Sciences, Nanjing (China)
Univ. of Oklahoma, Norman, OK (United States)
Univ. of Oklahoma, Norman, OK (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Tsinghua Univ., Beijing (China)

Sediment microbial fuel cells (SMFCs) can stimulate the degradation of polycyclic aromatic hydrocarbons in sediments, but the mechanism of this process is poorly understood at the microbial functional gene level. Here, the use of SMFC resulted in 92% benzo[a]pyrene (BaP) removal over 970 days relative to 54% in the controls. Sediment functions, microbial community structure, and network interactions were dramatically altered by the SMFC employment. Functional gene analysis showed that c-type cytochrome genes for electron transfer, aromatic degradation genes, and extracellular ligninolytic enzymes involved in lignin degradation were significantly enriched in bulk sediments during SMFC operation. Correspondingly, chemical analysis of the system showed that these genetic changes resulted in increases in the levels of easily oxidizable organic carbon and humic acids which may have resulted in increased BaP bioavailability and increased degradation rates. In conclusion, tracking microbial functional genes and corresponding organic matter responses should aid mechanistic understanding of BaP enhanced biodegradation by microbial electrochemistry and development of sustainable bioremediation strategies.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1567094

Journal Information:: Environmental Science and Technology, Vol. 51, Issue 15; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 53 works

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