Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.
Abstract
Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolerate large changes in pressure, temperature, and salinity. Here, we used a Halanaerobium strain isolated from a natural gas well in the Utica Point Pleasant formation to investigate metabolic and physiological responses to growth under high-pressure subsurface conditions. Laboratory incubations confirmed the ability of Halanaerobium congolense strain WG8 to grow under pressures representative of deep shale formations (21 to 48 MPa). Under these conditions, broad metabolic and physiological shifts were identified, including higher abundances of proteins associated with the production of extracellular polymeric substances. Confocal laser scanning microscopy indicated that extracellular polymeric substance (EPS) production was associated with greater cell aggregation when biomass was cultured at high pressure.
- Authors:
-
[5];
- The Ohio State Univ., Columbus, OH (United States)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- DowDuPont Industrial Biosciences, Wilmington, DE (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
- Univ. of New Hampshire, Durham, NH (United States)
- Colorado State Univ., Fort Collins, CO (United States)
- Chinese Academy of Sciences (CAS), Beijing (China)
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1567051
- Report Number(s):
- PNNL-SA-144542
Journal ID: ISSN 0099-2240
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Applied and Environmental Microbiology
- Additional Journal Information:
- Journal Volume: 85; Journal Issue: 12; Journal ID: ISSN 0099-2240
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Halanaerobium; Shale; Biofilms; High Pressure; Hydraulic fracturing; Metabolomics
Citation Formats
Booker, Anne E., Hoyt, David W., Meulia, Tea, Eder, Elizabeth, Nicora, Carrie D., Purvine, Samuel O., Daly, Rebecca A., Moore, Joseph D., Wunch, Kenneth, Pfiffner, Susan M., Lipton, Mary S., Mouser, Paula J., Wrighton, Kelly C., Wilkins, Michael J., and Liu, Shuang-Jiang. Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.. United States: N. p., 2019.
Web. doi:10.1128/AEM.00018-19.
Booker, Anne E., Hoyt, David W., Meulia, Tea, Eder, Elizabeth, Nicora, Carrie D., Purvine, Samuel O., Daly, Rebecca A., Moore, Joseph D., Wunch, Kenneth, Pfiffner, Susan M., Lipton, Mary S., Mouser, Paula J., Wrighton, Kelly C., Wilkins, Michael J., & Liu, Shuang-Jiang. Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.. United States. https://doi.org/10.1128/AEM.00018-19
Booker, Anne E., Hoyt, David W., Meulia, Tea, Eder, Elizabeth, Nicora, Carrie D., Purvine, Samuel O., Daly, Rebecca A., Moore, Joseph D., Wunch, Kenneth, Pfiffner, Susan M., Lipton, Mary S., Mouser, Paula J., Wrighton, Kelly C., Wilkins, Michael J., and Liu, Shuang-Jiang. Fri .
"Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.". United States. https://doi.org/10.1128/AEM.00018-19. https://www.osti.gov/servlets/purl/1567051.
@article{osti_1567051,
title = {Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.},
author = {Booker, Anne E. and Hoyt, David W. and Meulia, Tea and Eder, Elizabeth and Nicora, Carrie D. and Purvine, Samuel O. and Daly, Rebecca A. and Moore, Joseph D. and Wunch, Kenneth and Pfiffner, Susan M. and Lipton, Mary S. and Mouser, Paula J. and Wrighton, Kelly C. and Wilkins, Michael J. and Liu, Shuang-Jiang},
abstractNote = {Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolerate large changes in pressure, temperature, and salinity. Here, we used a Halanaerobium strain isolated from a natural gas well in the Utica Point Pleasant formation to investigate metabolic and physiological responses to growth under high-pressure subsurface conditions. Laboratory incubations confirmed the ability of Halanaerobium congolense strain WG8 to grow under pressures representative of deep shale formations (21 to 48 MPa). Under these conditions, broad metabolic and physiological shifts were identified, including higher abundances of proteins associated with the production of extracellular polymeric substances. Confocal laser scanning microscopy indicated that extracellular polymeric substance (EPS) production was associated with greater cell aggregation when biomass was cultured at high pressure.},
doi = {10.1128/AEM.00018-19},
journal = {Applied and Environmental Microbiology},
number = 12,
volume = 85,
place = {United States},
year = {Fri Apr 12 00:00:00 EDT 2019},
month = {Fri Apr 12 00:00:00 EDT 2019}
}
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Cited by: 13 works
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Figures / Tables:
FIG 1: H. congolense WG8 growth at high pressure. Growth rates (red) and corresponding cell densities (gray) across a pressure gradient (0.1 to 48 MPa) are shown. Growth rate and cell densities decreased as incubation pressure increased.
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Works referenced in this record:
TonB-Dependent Transporters: Regulation, Structure, and Function
journal, October 2010
- Noinaj, Nicholas; Guillier, Maude; Barnard,, Travis J.
- Annual Review of Microbiology, Vol. 64, Issue 1
Pressure effects on in vivo microbial processes
journal, March 2002
- Bartlett, D. H.
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1595, Issue 1-2
Biofilm formation by a Bacillus subtilis strain that produces -polyglutamate
journal, September 2006
- Morikawa, M.
- Microbiology, Vol. 152, Issue 9
Improved measurement of microbial activity in deep-sea sediments at in situ pressure and methane concentration: Measuring activity in deep-sea sediments
journal, October 2011
- Bowles, Marshall W.; Samarkin, Vladimir A.; Joye, Samantha B.
- Limnology and Oceanography: Methods, Vol. 9, Issue 10
Stress response of Escherichia coli to elevated hydrostatic pressure.
journal, November 1993
- Welch, T. J.; Farewell, A.; Neidhardt, F. C.
- Journal of Bacteriology, Vol. 175, Issue 22
Autotrophy at the thermodynamic limit of life: a model for energy conservation in acetogenic bacteria
journal, November 2014
- Schuchmann, Kai; Müller, Volker
- Nature Reviews Microbiology, Vol. 12, Issue 12
Biofilm-Related Diseases and Omics: Global Transcriptional Profiling of Enterococcus faecium Reveals Different Gene Expression Patterns in the Biofilm and Planktonic Cells
journal, October 2017
- Lim, Shu Yong; Teh, Cindy Shuan Ju; Thong, Kwai Lin
- OMICS: A Journal of Integrative Biology, Vol. 21, Issue 10
Bacterial biofilm shows persistent resistance to liquid wetting and gas penetration
journal, December 2010
- Epstein, A. K.; Pokroy, B.; Seminara, A.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 3
Current developments in marine microbiology: high-pressure biotechnology and the genetic engineering of piezophiles
journal, June 2015
- Zhang, Yu; Li, Xuegong; Bartlett, Douglas H.
- Current Opinion in Biotechnology, Vol. 33
Bacterial Communities Associated with Production Facilities of Two Newly Drilled Thermogenic Natural Gas Wells in the Barnett Shale (Texas, USA)
journal, May 2012
- Davis, James P.; Struchtemeyer, Christopher G.; Elshahed, Mostafa S.
- Microbial Ecology, Vol. 64, Issue 4
Resilience of planktonic and biofilm cultures to supercritical CO2
journal, December 2008
- Mitchell, Andrew C.; Phillips, Adrienne J.; Hamilton, Marty A.
- The Journal of Supercritical Fluids, Vol. 47, Issue 2
Pyruvate Ferredoxin Oxidoreductase and Its Radical Intermediate †
journal, June 2003
- Ragsdale, Stephen W.
- Chemical Reviews, Vol. 103, Issue 6
Ethylene Glycol Metabolism in the Acetogen Acetobacterium woodii
journal, January 2016
- Trifunović, Dragan; Schuchmann, Kai; Müller, Volker
- Journal of Bacteriology, Vol. 198, Issue 7
Microbial metabolisms in a 2.5-km-deep ecosystem created by hydraulic fracturing in shales
journal, September 2016
- Daly, Rebecca A.; Borton, Mikayla A.; Wilkins, Michael J.
- Nature Microbiology, Vol. 1, Issue 10
Influence of Anaerobiosis and Low Temperature on Bacillus cereus Growth, Metabolism, and Membrane Properties
journal, January 2012
- de Sarrau, Benoît; Clavel, Thierry; Clerté, Caroline
- Applied and Environmental Microbiology, Vol. 78, Issue 6
A role for TonB1 in biofilm formation and quorum sensing in Pseudomonas aeruginosa
journal, September 2007
- Abbas, Abdelhamid; Adams, Claire; Scully, Norma
- FEMS Microbiology Letters, Vol. 274, Issue 2
Polyhedral organelles compartmenting bacterial metabolic processes
journal, March 2006
- Bobik, Thomas A.
- Applied Microbiology and Biotechnology, Vol. 70, Issue 5
Heat Stress Dictates Microbial Lipid Composition along a Thermal Gradient in Marine Sediments
journal, August 2017
- Sollich, Miriam; Yoshinaga, Marcos Y.; Häusler, Stefan
- Frontiers in Microbiology, Vol. 8
Spermidine promotes Bacillus subtilis biofilm formation by activating expression of the matrix regulator slrR
journal, May 2017
- Hobley, Laura; Li, Bin; Wood, Jennifer L.
- Journal of Biological Chemistry, Vol. 292, Issue 29
Acetate-Enhanced Corrosion of Carbon Steel—Further Factors in Oilfield Environments
journal, March 2007
- Pletcher, D.; Sidorin, D.; Hedges, B.
- CORROSION, Vol. 63, Issue 3
Absence of TolC Impairs Biofilm Formation in Actinobacillus pleuropneumoniae by Reducing Initial Attachment
journal, September 2016
- Li, Ying; Cao, Sanjie; Zhang, Luhua
- PLOS ONE, Vol. 11, Issue 9
The PduM Protein Is a Structural Component of the Microcompartments Involved in Coenzyme B12-Dependent 1,2-Propanediol Degradation by Salmonella enterica
journal, February 2012
- Sinha, S.; Cheng, S.; Fan, C.
- Journal of Bacteriology, Vol. 194, Issue 8
Morphological and proteomic analysis of biofilms from the Antarctic archaeon, Halorubrum lacusprofundi
journal, November 2016
- Liao, Y.; Williams, T. J.; Ye, J.
- Scientific Reports, Vol. 6, Issue 1
Predominance and Metabolic Potential of Halanaerobium spp. in Produced Water from Hydraulically Fractured Marcellus Shale Wells
journal, February 2017
- Lipus, Daniel; Vikram, Amit; Ross, Daniel
- Applied and Environmental Microbiology, Vol. 83, Issue 8
Temporal Changes in Microbial Ecology and Geochemistry in Produced Water from Hydraulically Fractured Marcellus Shale Gas Wells
journal, May 2014
- Cluff, Maryam A.; Hartsock, Angela; MacRae, Jean D.
- Environmental Science & Technology, Vol. 48, Issue 11
High-pressure tolerance in Halobacterium salinarum NRC-1 and other non-piezophilic prokaryotes
journal, January 2012
- Kish, Adrienne; Griffin, Patrick L.; Rogers, Karyn L.
- Extremophiles, Vol. 16, Issue 2
Biomass, bioactivity and biodiversity: microbial ecology of the deep subsurface: analysis of ester-linked phospholipid fatty acids
journal, July 1997
- Ringelberg, David B.; Sutton, Susan; White, David C.
- FEMS Microbiology Reviews, Vol. 20, Issue 3-4
Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains
journal, January 2012
- Collas, Florent; Kuit, Wouter; Clément, Benjamin
- AMB Express, Vol. 2, Issue 1
Quantitative proteomic analysis of distinct mammalian Mediator complexes using normalized spectral abundance factors
journal, November 2006
- Paoletti, A. C.; Parmely, T. J.; Tomomori-Sato, C.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 50
Mechanisms of biofilm resistance to antimicrobial agents
journal, January 2001
- Mah, Thien-Fah C.; O'Toole, George A.
- Trends in Microbiology, Vol. 9, Issue 1
Classic Spotlight: Hfq, from a Specific Host Factor for Phage Replication to a Global Player in Riboregulation
journal, August 2016
- Becker, Anke
- Journal of Bacteriology, Vol. 198, Issue 17
Role and Regulation of Fatty Acid Biosynthesis in the Response of Shewanella piezotolerans WP3 to Different Temperatures and Pressures
journal, February 2009
- Wang, F.; Xiao, X.; Ou, H. -Y.
- Journal of Bacteriology, Vol. 191, Issue 8
Hydraulic fracturing offers view of microbial life in the deep terrestrial subsurface
journal, August 2016
- Mouser, Paula J.; Borton, Mikayla; Darrah, Thomas H.
- FEMS Microbiology Ecology, Vol. 92, Issue 11
Discovery of an archetypal protein transport system in bacterial outer membranes
journal, April 2012
- Selkrig, Joel; Mosbahi, Khedidja; Webb, Chaille T.
- Nature Structural & Molecular Biology, Vol. 19, Issue 5
Differential Gene Expression Profiling of Staphylococcus aureus Cultivated under Biofilm and Planktonic Conditions
journal, May 2005
- Resch, A.; Rosenstein, R.; Nerz, C.
- Applied and Environmental Microbiology, Vol. 71, Issue 5
High pressure effects on the activity of glycolytic enzymes
journal, February 1975
- Schmid, G.; Lüdemann, H. -D.; Jaenicke, R.
- Biophysical Chemistry, Vol. 3, Issue 1
Pullulanase: Role in Starch Hydrolysis and Potential Industrial Applications
journal, January 2012
- Hii, Siew Ling; Tan, Joo Shun; Ling, Tau Chuan
- Enzyme Research, Vol. 2012
Transcriptional Analysis of Biofilm Formation Processes in the Anaerobic, Hyperthermophilic Bacterium Thermotoga maritima
journal, October 2004
- Pysz, M. A.; Conners, S. B.; Montero, C. I.
- Applied and Environmental Microbiology, Vol. 70, Issue 10, p. 6098-6112
Involvement of Iron in Biofilm Formation by Staphylococcus aureus
journal, March 2012
- Lin, Mei-Hui; Shu, Jwu-Ching; Huang, Hsiu-Yun
- PLoS ONE, Vol. 7, Issue 3
Pores in Marcellus Shale: A Neutron Scattering and FIB-SEM Study
journal, February 2015
- Gu, Xin; Cole, David R.; Rother, Gernot
- Energy & Fuels, Vol. 29, Issue 3
Pseudomonas aeruginosa Displays Multiple Phenotypes during Development as a Biofilm
journal, February 2002
- Sauer, K.; Camper, A. K.; Ehrlich, G. D.
- Journal of Bacteriology, Vol. 184, Issue 4
Microbial diversity and methanogenic activity of Antrim Shale formation waters from recently fractured wells
journal, January 2013
- Wuchter, Cornelia; Banning, Erin; Mincer, Tracy J.
- Frontiers in Microbiology, Vol. 4
Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes
journal, December 1998
- Daniel, Rolf; Bobik, Thomas A.; Gottschalk, Gerhard
- FEMS Microbiology Reviews, Vol. 22, Issue 5, p. 553-566
Microbial Community Changes in Hydraulic Fracturing Fluids and Produced Water from Shale Gas Extraction
journal, October 2013
- Murali Mohan, Arvind; Hartsock, Angela; Bibby, Kyle J.
- Environmental Science & Technology, Vol. 47, Issue 22
Micro/Nano-pore Network Analysis of Gas Flow in Shale Matrix
journal, August 2015
- Zhang, Pengwei; Hu, Liming; Meegoda, Jay N.
- Scientific Reports, Vol. 5, Issue 1
Understanding the Mechanism of B 12 -Dependent Diol Dehydratase: A Synergistic Retro-Push−Pull Proposal
journal, February 2001
- Smith, David M.; Golding, Bernard T.; Radom, Leo
- Journal of the American Chemical Society, Vol. 123, Issue 8
Targeted Profiling: Quantitative Analysis of 1 H NMR Metabolomics Data
journal, July 2006
- Weljie, Aalim M.; Newton, Jack; Mercier, Pascal
- Analytical Chemistry, Vol. 78, Issue 13
Coupled laboratory and field investigations resolve microbial interactions that underpin persistence in hydraulically fractured shales
journal, June 2018
- Borton, Mikayla A.; Hoyt, David W.; Roux, Simon
- Proceedings of the National Academy of Sciences, Vol. 115, Issue 28
Sulfide Generation by Dominant Halanaerobium Microorganisms in Hydraulically Fractured Shales
journal, July 2017
- Booker, Anne E.; Borton, Mikayla A.; Daly, Rebecca A.
- mSphere, Vol. 2, Issue 4
Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum
journal, January 2015
- Biswas, Ranjita; Zheng, Tianyong; Olson, Daniel G.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Enhancing the Adaptability of the Deep-Sea Bacterium Shewanella piezotolerans WP3 to High Pressure and Low Temperature by Experimental Evolution under H 2 O 2 Stress
journal, December 2017
- Xie, Zhe; Jian, Huahua; Jin, Zheng
- Applied and Environmental Microbiology, Vol. 84, Issue 5
Biofilm Matrix and Its Regulation in Pseudomonas aeruginosa
journal, October 2013
- Wei, Qing; Ma, Luyan
- International Journal of Molecular Sciences, Vol. 14, Issue 10
High hydrostatic pressure adaptive strategies in an obligate piezophile Pyrococcus yayanosii
journal, June 2016
- Michoud, Grégoire; Jebbar, Mohamed
- Scientific Reports, Vol. 6, Issue 1
Growth Rates Made Easy
journal, October 2013
- Hall, B. G.; Acar, H.; Nandipati, A.
- Molecular Biology and Evolution, Vol. 31, Issue 1
Identification of Poly- N- acetylglucosamine as a Major Polysaccharide Component of the Bacillus subtilis Biofilm Matrix
journal, June 2015
- Roux, Damien; Cywes-Bentley, Colette; Zhang, Yi-Fan
- Journal of Biological Chemistry, Vol. 290, Issue 31
Reduced TCA cycle rates at high hydrostatic pressure hinder hydrocarbon degradation and obligate oil degraders in natural, deep-sea microbial communities
journal, December 2018
- Scoma, Alberto; Heyer, Robert; Rifai, Ridwan
- The ISME Journal, Vol. 13, Issue 4
Self-induced mechanical stress can trigger biofilm formation in uropathogenic Escherichia coli
journal, October 2018
- Chu, Eric K.; Kilic, Onur; Cho, Hojung
- Nature Communications, Vol. 9, Issue 1
Protein Expression by Streptococcus mutans during Initial Stage of Biofilm Formation
journal, June 2004
- Welin, J.; Wilkins, J. C.; Beighton, D.
- Applied and Environmental Microbiology, Vol. 70, Issue 6
Metabolic Capability of a Predominant Halanaerobium sp. in Hydraulically Fractured Gas Wells and Its Implication in Pipeline Corrosion
journal, June 2016
- Liang, Renxing; Davidova, Irene A.; Marks, Christopher R.
- Frontiers in Microbiology, Vol. 7
The relationship between glycogen synthesis, biofilm formation and virulence in Salmonella enteritidis
journal, October 2000
- Bonafonte, M. Angeles; Solano, Cristina; Sesma, Begoña
- FEMS Microbiology Letters, Vol. 191, Issue 1
The Phosphoenolpyruvate Phosphotransferase System Regulates Vibrio cholerae Biofilm Formation through Multiple Independent Pathways
journal, April 2010
- Houot, L.; Chang, S.; Pickering, B. S.
- Journal of Bacteriology, Vol. 192, Issue 12
Bacterial diguanylate cyclases: Structure, function and mechanism in exopolysaccharide biofilm development
journal, January 2015
- Whiteley, Chris G.; Lee, Duu-Jong
- Biotechnology Advances, Vol. 33, Issue 1
Pressure as an environmental parameter for microbial life — A review
journal, December 2013
- Picard, Aude; Daniel, Isabelle
- Biophysical Chemistry, Vol. 183
Biofilm Matrix Exoproteins Induce a Protective Immune Response against Staphylococcus aureus Biofilm Infection
journal, December 2013
- Gil, Carmen; Solano, Cristina; Burgui, Saioa
- Infection and Immunity, Vol. 82, Issue 3
Vibrio fischeri 54 Controls Motility, Biofilm Formation, Luminescence, and Colonization
journal, April 2004
- Wolfe, A. J.; Millikan, D. S.; Campbell, J. M.
- Applied and Environmental Microbiology, Vol. 70, Issue 4
A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8
Bacterial lipid membranes as promising targets to fight antimicrobial resistance, molecular foundations and illustration through the renewal of aminoglycoside antibiotics and emergence of amphiphilic aminoglycosides
journal, January 2016
- Mingeot-Leclercq, Marie-Paule; Décout, Jean-Luc
- MedChemComm, Vol. 7, Issue 4
Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion
journal, May 1992
- Mack, D.; Siemssen, N.; Laufs, R.
- Infection and Immunity, Vol. 60, Issue 5
Monounsaturated but Not Polyunsaturated Fatty Acids Are Required for Growth of the Deep-Sea Bacterium Photobacterium profundum SS9 at High Pressure and Low Temperature
journal, April 1999
- Allen, Eric E.; Facciotti, Daniel; Bartlett, Douglas H.
- Applied and Environmental Microbiology, Vol. 65, Issue 4
Pyruvate Ferredoxin Oxidoreductase and Its Radical Intermediate
journal, August 2003
- Ragsdale, Stephen W.
- ChemInform, Vol. 34, Issue 32
Adaptive Processes Change as Multiple Functions Evolve
journal, March 2021
- Mira, Portia M.; Østman, Bjørn; Guzman-Cole, Candace
- Antimicrobial Agents and Chemotherapy, Vol. 65, Issue 4
A Rapid Method of Total Lipid Extraction and Purification
journal, January 1959
- Bligh, E. G.; Dyer, W. J.
- Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 1
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- Biotechnology Letters, Vol. 42, Issue 3
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journal, June 2019
- Evans, Morgan V.; Getzinger, Gordon; Luek, Jenna L.
- The ISME Journal, Vol. 13, Issue 11
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