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Title: Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR

Abstract

Lignin is a complex aromatic biopolymer that strengthens and waterproofs plant secondary cell walls, enabling mechanical stability in trees and long-distance water transport in xylem. Lignin removal is a key step in paper production and biomass conversion to biofuels, motivating efforts to re-engineer lignin biosynthesis. However, the physical nature of lignin’s interactions with wall polysaccharides is not well understood. Here we show that lignin self-aggregates to form highly hydrophobic and dynamically unique nanodomains, with extensive surface contacts to xylan. Solid-state NMR spectroscopy of intact maize stems, supported by dynamic nuclear polarization, reveals that lignin has abundant electrostatic interactions with the polar motifs of xylan. Lignin preferentially binds xylans with 3-fold or distorted 2-fold helical screw conformations, indicative of xylans not closely associated with cellulose. These findings advance our knowledge of the molecular-level organization of lignocellulosic biomass, providing the structural foundation for optimization of post-harvest processing for biofuels and biomaterials.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [2];  [3]; ORCiD logo [1]
  1. Louisiana State Univ., Baton Rouge, LA (United States)
  2. Florida State Univ., Tallahassee, FL (United States). National High Magnetic Field Lab. (MagLab)
  3. Pennsylvania State Univ., University Park, PA (United States)
Publication Date:
Research Org.:
Energy Frontier Research Centers (EFRC) (United States). Center for Lignocellulose Structure and Formation (CLSF); Pennsylvania State Univ., University Park, PA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1566610
Grant/Contract Number:  
SC0001090
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; biofuels (including algae and biomass); bio-inspired; membrane; carbon sequestration; materials and chemistry by design; synthesis (self-assembly)

Citation Formats

Kang, Xue, Kirui, Alex, Dickwella Widanage, Malitha C., Mentink-Vigier, Frederic, Cosgrove, Daniel J., and Wang, Tuo. Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR. United States: N. p., 2019. Web. doi:10.1038/s41467-018-08252-0.
Kang, Xue, Kirui, Alex, Dickwella Widanage, Malitha C., Mentink-Vigier, Frederic, Cosgrove, Daniel J., & Wang, Tuo. Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR. United States. doi:10.1038/s41467-018-08252-0.
Kang, Xue, Kirui, Alex, Dickwella Widanage, Malitha C., Mentink-Vigier, Frederic, Cosgrove, Daniel J., and Wang, Tuo. Mon . "Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR". United States. doi:10.1038/s41467-018-08252-0. https://www.osti.gov/servlets/purl/1566610.
@article{osti_1566610,
title = {Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR},
author = {Kang, Xue and Kirui, Alex and Dickwella Widanage, Malitha C. and Mentink-Vigier, Frederic and Cosgrove, Daniel J. and Wang, Tuo},
abstractNote = {Lignin is a complex aromatic biopolymer that strengthens and waterproofs plant secondary cell walls, enabling mechanical stability in trees and long-distance water transport in xylem. Lignin removal is a key step in paper production and biomass conversion to biofuels, motivating efforts to re-engineer lignin biosynthesis. However, the physical nature of lignin’s interactions with wall polysaccharides is not well understood. Here we show that lignin self-aggregates to form highly hydrophobic and dynamically unique nanodomains, with extensive surface contacts to xylan. Solid-state NMR spectroscopy of intact maize stems, supported by dynamic nuclear polarization, reveals that lignin has abundant electrostatic interactions with the polar motifs of xylan. Lignin preferentially binds xylans with 3-fold or distorted 2-fold helical screw conformations, indicative of xylans not closely associated with cellulose. These findings advance our knowledge of the molecular-level organization of lignocellulosic biomass, providing the structural foundation for optimization of post-harvest processing for biofuels and biomaterials.},
doi = {10.1038/s41467-018-08252-0},
journal = {Nature Communications},
number = 1,
volume = 10,
place = {United States},
year = {2019},
month = {1}
}

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Cited by: 9 works
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Works referenced in this record:

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