The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor
Abstract
Neurotransmitter:sodium symporters (NSSs) in the SLC6 family terminate neurotransmission by coupling the thermodynamically favorable transport of ions to the thermodynamically unfavorable transport of neurotransmitter back into presynaptic neurons. Results from many structural, functional, and computational studies on LeuT, a bacterial NSS homolog, have provided critical insight into the mechanism of sodium-coupled transport, but the mechanism underlying substrate-specific transport rates is still not understood. We present a combination of molecular dynamics simulations, single-molecule fluorescence resonance energy transfer (smFRET) imaging, and measurements of Na+ binding and substrate transport that reveals an allosteric substrate specificity mechanism. In this mechanism, residues F259 and I359 in the substrate binding pocket couple the binding of substrate to Na+ release from the Na2 site by allosterically modulating the stability of a partially open, inward-facing state. We propose a model for transport selectivity in which residues F259 and I359 act as a volumetric sensor that inhibits the transport of bulky amino acids.
- Authors:
-
- Weill Cornell Medicine, New York, NY (United States)
- Columbia Univ., New York, NY (United States); New York State Psychiatric Inst. New York, NY (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC) (OLCF); Univ. of California, Oakland, CA (United States); UT-Battelle LLC/ORNL, Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Advanced Scientific Computing Research (ASCR); National Institutes of Health (NIH); National Science Foundation (NSF)
- OSTI Identifier:
- 1565765
- Grant/Contract Number:
- AC02-05CH11231; AC05-00OR22725; R21-MH099491; U54-GM087510; P01-DA012408; R01-DA041510; F31-DA035533; ACI-1053575; R01-GM116961
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 116; Journal Issue: 32; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
LeVine, Michael V., Terry, Daniel S., Khelashvili, George, Siegel, Zarek S., Quick, Matthias, Javitch, Jonathan A., Blanchard, Scott C., and Weinstein, Harel. The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor. United States: N. p., 2019.
Web. doi:10.1073/pnas.1903020116.
LeVine, Michael V., Terry, Daniel S., Khelashvili, George, Siegel, Zarek S., Quick, Matthias, Javitch, Jonathan A., Blanchard, Scott C., & Weinstein, Harel. The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor. United States. https://doi.org/10.1073/pnas.1903020116
LeVine, Michael V., Terry, Daniel S., Khelashvili, George, Siegel, Zarek S., Quick, Matthias, Javitch, Jonathan A., Blanchard, Scott C., and Weinstein, Harel. Fri .
"The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor". United States. https://doi.org/10.1073/pnas.1903020116. https://www.osti.gov/servlets/purl/1565765.
@article{osti_1565765,
title = {The allosteric mechanism of substrate-specific transport in SLC6 is mediated by a volumetric sensor},
author = {LeVine, Michael V. and Terry, Daniel S. and Khelashvili, George and Siegel, Zarek S. and Quick, Matthias and Javitch, Jonathan A. and Blanchard, Scott C. and Weinstein, Harel},
abstractNote = {Neurotransmitter:sodium symporters (NSSs) in the SLC6 family terminate neurotransmission by coupling the thermodynamically favorable transport of ions to the thermodynamically unfavorable transport of neurotransmitter back into presynaptic neurons. Results from many structural, functional, and computational studies on LeuT, a bacterial NSS homolog, have provided critical insight into the mechanism of sodium-coupled transport, but the mechanism underlying substrate-specific transport rates is still not understood. We present a combination of molecular dynamics simulations, single-molecule fluorescence resonance energy transfer (smFRET) imaging, and measurements of Na+ binding and substrate transport that reveals an allosteric substrate specificity mechanism. In this mechanism, residues F259 and I359 in the substrate binding pocket couple the binding of substrate to Na+ release from the Na2 site by allosterically modulating the stability of a partially open, inward-facing state. We propose a model for transport selectivity in which residues F259 and I359 act as a volumetric sensor that inhibits the transport of bulky amino acids.},
doi = {10.1073/pnas.1903020116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 32,
volume = 116,
place = {United States},
year = {2019},
month = {7}
}
Web of Science
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