Monodeuterated Methane, an Isotopic Tool To Assess Biological Methane Metabolism Rates

Marlow, Jeffrey J.; Steele, Joshua A.; Ziebis, Wiebke; Scheller, Silvan; Case, David; Reynard, Linda M.; Orphan, Victoria J.; Green Tringe, Susannah

doi:10.1128/mspheredirect.00309-17

Title: Monodeuterated Methane, an Isotopic Tool To Assess Biological Methane Metabolism Rates

Journal Article · 23 August 2017 · mSphere

DOI: https://doi.org/10.1128/mspheredirect.00309-17 · OSTI ID:1565620

Marlow, Jeffrey J. ^[1]; Steele, Joshua A. ^[1]; Ziebis, Wiebke ^[2]; Scheller, Silvan ^[1]; Case, David ^[1]; Reynard, Linda M. ^[3]; Orphan, Victoria J. ^[1]; Green Tringe, Susannah ^[4]

California Inst. of Technology (CalTech), Pasadena, CA (United States). Division of Geological and Planetary Sciences
Univ. of Southern California, Los Angeles, CA (United States). Dept. of Biological Science
Harvard Univ., Cambridge, MA (United States), Dept. of Human Evolutionary Biology
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); editor

Biological methane oxidation is a globally relevant process that mediates the flux of an important greenhouse gas through both aerobic and anaerobic metabolic pathways. However, measuring these metabolic rates presents many obstacles, from logistical barriers to regulatory hurdles and poor precision. Here we present a new approach for investigating microbial methane metabolism based on hydrogen atom dynamics, which is complementary to carbon-focused assessments of methanotrophy. The method uses monodeuterated methane (CH₃D) as a metabolic substrate, quantifying the aqueous D/H ratio over time using off-axis integrated cavity output spectroscopy. This approach represents a nontoxic, comparatively rapid, and straightforward approach that supplements existing radiotopic and stable carbon isotopic methods; by probing hydrogen atoms, it offers an additional dimension for examining rates and pathways of methane metabolism. We provide direct comparisons between the CH₃D procedure and the well-established¹⁴CH₄ radiotracer method for several methanotrophic systems, including type I and II aerobic methanotroph cultures and methane-seep sediment slurries and carbonate rocks under anoxic and oxic incubation conditions. In all applications tested, methane consumption values calculated via the CH₃D method were directly and consistently proportional to¹⁴C radiolabel-derived methane oxidation rates. We also employed this method in a nontraditional experimental setup, using flexible, gas-impermeable bags to investigate the role of pressure on seep sediment methane oxidation rates. Results revealed an 80% increase over atmospheric pressure in methanotrophic rates the equivalent of ~900-m water depth, highlighting the importance of this parameter on methane metabolism and exhibiting the flexibility of the newly described method.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Aeronautics and Space Administration (NASA); Gordon and Betty Moore Foundation (GBMF)

Grant/Contract Number:: SC001057

OSTI ID:: 1565620

Journal Information:: mSphere, Vol. 2, Issue 4; ISSN 2379-5042

Publisher:: American Society for MicrobiologyCopyright Statement

Country of Publication:: United States

Language:: English

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